Effect of Improved Trichoderma harzianum on Growth and Resistance Promotion in Bean Plant

Abstract

Different species of Trichoderma harzianum have been used mainly as an antagonist against a number of plant diseases. There is a direct relationship between the biocontrol potential of these fungi and the rate of enzymatic degradation of the cell walls of pathogens. Following the successful production of chimeric chit 42 via protein engineering in the previous work of authors, the resulting recombinant protein was transferred to T. harzianum. In this study presence and stability of chimeric Chitinase (Chit42-ChBD) was verified then growth stimulating and biocontrol potential of the six improved isolates were evaluated on bean plants under laboratory and greenhouse conditions compared to the wild type. Among engineered isolates, Chit42-ChBD3 with 53.83% growth inhibition on Rhizoctonia showed the highest growth rate and sporulation compared to the wild type in the dual culture test. The results showed that ChBD15 and ChBD13 had better performance in terms of increasing nutrient uptake and root growth thereby enhancing the growth HIGHLIGHTS• Trichoderma inoculation increased the total biomass, seedling height, root length, root area, root diameter, number of root tips, and number growth potential of seedlings.• Trichoderma inoculation increases the resistance of bean plant to Rhizoctonia pathogen.• Among improved isolates of Trichoderma, the ChBD13 and ChBD15 showed better performance in terms of plant growth.

Application of Trichoderma harzianum and Bacillus thuringiensis in increasing plant growth of Aceh patchouli (Pogostemon cablin Benth)

“…T. harzianum acts as a high growth regulator that stimulates the growth of patchouli plants and finally patchouli plants can grow optimally [4]. T. harzianum can accelerate plant growth by producing IAA compounds and increasing plant defense mechanisms [14].…”