Dietary Brown Propolis Extract Modulated Nonspecific Immune System and Intestinal Morphology of Pacu Piaractus mesopotamicus

Abstract

The immunomodulatory and growth promoter effects of brown propolis ethanolic extract (PEE) were determined in pacu. Fish (28.4±0.4 g) were randomly distributed into 12 polyethylene circular tanks (250 L, 30 fish per tank) and fed for 60 days with a commercial diet (32% crude protein) supplemented with 0.0, 1.5, 3.0 and 4.5% (v/w) of PEE in a totally randomized experimental design (n=3). Fish organic defense cells numbers such as thrombocytes and neutrophils increased (p<0.05) in fish fed 3.0% dietary PEE. Serum lysozyme concentration also increased (p<0.05) in fish fed 1.5 and 3.0% PEE supplementation when compared to unsupplemented fish. Growth parameters were not influenced (p>0.05) by treatments. Moreover, dietary PEE decreased (p<0.05) fish intestinal muscular thickness when compared to control treatment. Intestine villi height also significantly decreased in fish fed 3.0% PEE. Long term dietary PEE at 3.0% supplementation level modulated fish nonspecific immune system and it is a non-toxic substance for pacu.

Superabsorbent biodegradable CMC membranes loaded with propolis: Peppas-Sahlin kinetics release

“…Regarding brown propolis, [46] low amounts of propolis extract were required to scavenge 50% of DPPH (from DMT (3.7 μg/mL) to USI (4.1 μg/mL)). The ABTS scavenging activity was in the expected range, [47] from 91 mmol trolox/100mg of propolis (DM) up to 103 mmol trolox/100mg of propolis (USI). DMT and USI techniques presented high FRAP results (264 mmol Fe(II)/100 mg propolis extract (DM) -372 mmol Fe(II)/100 mg propolis extract (USI)), although these values can be considered low for propolis extracts.…”