In vitro Antioxidant Activity and In vivo Anti-inflammatory Effect of Ricinus communis (L.) and Withania somnifera (L.) Hydroalcoholic Extracts in Rats

Hussain, Asif; Aslam, Bilal; Muhammad, Faqir; Faisal, Muhammad Naeem

doi:10.1590/1678-4324-2021200783

Cited by 7 publications

(4 citation statements)

References 39 publications

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“…Anti-edematogenic activity was evaluated through the xylene-induced ear edema model of rats [ 36 ]. Seven groups of rats ( n = 6) were pretreated with free compounds and nanoparticles ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Anti-Pyretic, Analgesic, and Anti-Inflammatory Activities of Meloxicam and Curcumin Co-Encapsulated PLGA Nanoparticles in Acute Experimental Models

Aslam,

Hussain,

Bari

et al. 2023

Metabolites

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Herein, we evaluated the in vivo effects of meloxicam and curcumin co-encapsulated PLGA nanoparticles in experimental acute models of pyrexia, nociception, and inflammation. Seven groups (n = 6) were designed for each investigation and pretreated intraperitoneally (i.p.): the control group, meloxicam (4 mg/kg b.w.), curcumin (15 mg/kg b.w.), and equivalent content containing PLGA capped nanoparticles of meloxicam (Mlx-NP) and curcumin (Cur-NP) alone and in combination (Mlx-Cur-NP; at two doses). The results showed that PLGA encapsulation significantly (p ≤ 0.05) improved the in vivo activities of each compound. Furthermore, co-encapsulation of meloxicam and curcumin potentiated the anti-pyretic effect on yeast-induced pyretic rats, anti-nociceptive effect on nociception induced in rats by formalin and heat, and anti-edematogenic activity in xylene-induced ear edema in rats in a dose-dependent manner. In carrageenan-induced paw inflammation in rats, meloxicam and curcumin co-loading (Mlx-Cur-NP) resulted in significant (p ≤ 0.05) inhibition of paw inflammation, reduction in TNF-α and PGE2 levels, downregulation of expressions of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6), as well as a decrease in histopathological changes and TNF-α immunoexpression in paw tissues. Moreover, Mlx-Cur-NP demonstrated noteworthy potentiation in pharmacological effects compared to free compounds and mono-compound-loaded nanoparticles. Thus, the association of meloxicam with curcumin in a biodegradable nanocarrier system could provide a promising anti-pyretic, anti-nociceptive, and anti-inflammatory therapeutic approach for acute conditions.

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Section: Methodsmentioning

confidence: 99%

Anti-Pyretic, Analgesic, and Anti-Inflammatory Activities of Meloxicam and Curcumin Co-Encapsulated PLGA Nanoparticles in Acute Experimental Models

Aslam,

Hussain,

Bari

et al. 2023

Metabolites

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“…The Folin-Ciocalteu method was adopted to quantify the total phenolic content (TPC) of NSE [23]. Briefly, 50 µL of extract was combined with 500 µL of Folin-Ciocalteu reagent and 1.5 mL of 20% Na2CO3 solution.…”

Section: Estimation Of Total Phenolic and Flavonoid Contentsmentioning

confidence: 99%

Nigella sativa L. Attenuates Oxidative Stress, Inflammation and Apoptosis in Concanavalin A-induced Acute Immunological Liver Damage in Mice

Mushtaq,

Aslam,

Faisal

et al. 2024

Braz. arch. biol. technol.

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Liver's contribution to innate immunity is eminent. However, uncontrolled inflammatory conditions predispose the liver to immune-mediated injury. Nigella sativa L. is traditionally implicated in infectious, inflammatory, metabolic and hepatorenal complications. This study aimed to evaluate the protective role of N. sativa seed extract (NSE) against concanavalin A (ConA)-induced acute immunological liver injury in mice. In vitro, NSE was subjected to quantitative phytochemical characterization and 1,1-diphenyl-2-picryhydrazyl (DPPH) analysis. In vivo, male Balb/c mice were pretreated with NSE (100, 200 and 400 mg/kg/day, p.o.) and pioglitazone (5 mg/kg/day, p.o.) for seven consecutive days. A single dose of ConA (12 mg/kg, i.v.) was injected and samples were collected for biochemical, histopathological and qRT-PCR analyses after 8 h of ConA injection. In vitro analysis showed considerable quantities of polyphenols and significant DPPH scavenging ability of NSE. In mice, ConA resulted in a significant (p<0.05) increase in liver injury markers (ALT, AST, ALP and TBil) and hepatic oxidative stress (SOD, CAT and MDA). Also, a substantial elevation of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) in liver tissues was noticed. Furthermore, ConA HIGHLIGHTS• Nigella sativa seed extract (NSE) possess significant polyphenol contents and antioxidant potential.• NSE attenuated concanavalin A-induced liver injury and oxidative stress.• NSE suppressed liver inflammation through modulating PPARγ/JAK2/STAT3 pathway.• NSE inhibited hepatocyte apoptosis via downregulating Bcl-2/Bax/Caspase-9 pathway.

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“…Histopathological studies revealed that this extract of R. communis was able to counteract synovial inflammatory infiltration, bone resorption and pannus build‐up. Radiological data suggested that the plant also ameliorated joint space obstruction, ankylosis, tissue swelling and osteolysis (Hussain et al, 2021). So, this herb holds substantial potential in alleviating the symptoms of RA.…”

Section: Herbal Interventions With Potential Anti‐rheumatic Activitymentioning

confidence: 99%

Comprehensive insights into rheumatoid arthritis: Pathophysiology, current therapies and herbal alternatives for effective disease management

Chatterjee,

Jayaprakasan,

Chakrabarty

et al. 2024

Phytotherapy Research

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Rheumatoid arthritis is a chronic autoimmune inflammatory disease characterized by immune response overexpression, causing pain and swelling in the synovial joints. This condition is caused by auto‐reactive antibodies that attack self‐antigens due to their incapacity to distinguish between self and foreign molecules. Dysregulated activity within numerous signalling and immunological pathways supports the disease's development and progression, elevating its complexity. While current treatments provide some alleviation, their effectiveness is accompanied by a variety of adverse effects that are inherent in conventional medications. As a result, there is a deep‐rooted necessity to investigate alternate therapeutic strategies capable of neutralizing these disadvantages. Medicinal herbs display a variety of potent bioactive phytochemicals that are effective in the complementary management of disease, thus generating an enormous potency for the researchers to delve deep into the development of novel phytomedicine against autoimmune diseases, although additional evidence and understanding are required in terms of their efficacy and pharmacodynamic mechanisms. This literature‐based review highlights the dysregulation of immune tolerance in rheumatoid arthritis, analyses the pathophysiology, elucidates relevant signalling pathways involved, evaluates present and future therapy options and underscores the therapeutic attributes of a diverse array of medicinal herbs in addressing this severe disease.

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In vitro Antioxidant Activity and In vivo Anti-inflammatory Effect of Ricinus communis (L.) and Withania somnifera (L.) Hydroalcoholic Extracts in Rats

Cited by 7 publications

References 39 publications

Anti-Pyretic, Analgesic, and Anti-Inflammatory Activities of Meloxicam and Curcumin Co-Encapsulated PLGA Nanoparticles in Acute Experimental Models

Anti-Pyretic, Analgesic, and Anti-Inflammatory Activities of Meloxicam and Curcumin Co-Encapsulated PLGA Nanoparticles in Acute Experimental Models

Nigella sativa L. Attenuates Oxidative Stress, Inflammation and Apoptosis in Concanavalin A-induced Acute Immunological Liver Damage in Mice

Comprehensive insights into rheumatoid arthritis: Pathophysiology, current therapies and herbal alternatives for effective disease management

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