Isolation and Characterization of Alkaline Pectinase Productive Bacillus tropicus from Fruit and Vegetable Waste Dump Soil

Thakur, Pitambri; Singh, Abhishek Kumar; Mukherjee, Gunjan

doi:10.1590/1678-4324-2021200319

Cited by 8 publications

(4 citation statements)

References 40 publications

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“…In this study, enzyme production was observed at temperatures ranging from 30 to 60 • C with pectinase maximum production at 30 • C with the enzymatic activity of 8.56 UI (Figure 4C). The findings correspond with those by Tepe and Dursun [87], which showed the ideal temperature for pectinase production by Bacillus pumilus at 30 • C; Ibrahim et al [88] and Thakur et al [89] also recorded similar findings for Aspergillus niger HFD5A-1 and for Bacillus tropicus at 37 • C, respectively.…”

Section: Effect Of Temperaturesupporting

confidence: 89%

Screening and Production of Industrially Relevant Enzymes by Bacillus paranthracis Strain MHDS3, a Potential Probiotic

Tshisikhawe,

Diale,

Abrahams

et al. 2023

Fermentation

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The digestive process and intestinal protein absorption are influenced by a variety of factors. Due to their numerous health advantages, including potential favorable effects on protein digestion and absorption, probiotics have gained increased attention in recent years. Probiotics can control the intestinal microflora, which in turn affects the intestinal bacteria responsible for proteolysis. Additionally, certain probiotics can release exoenzymes that aid in the digestion of proteins and others can stimulate the host’s digestive protease and peptidase activity. By boosting transport and enhancing the epithelium’s capacity for absorption, probiotics can also improve the absorption of tiny peptides and amino acids as well as lessen detrimental protein fermentation, which lowers the toxicity of metabolites. The present study explored the production of enzymes by Bacillus paranthracis strain MHDS3, a probiotic candidate isolated from Pellaea calomelanos. Bacillus paranthracis displayed enzyme activities of amylase (31,788.59 IU), cellulase (4487.486 IU), and pectinase (13.98986 IU) through submerged fermentation. The CAZyme analysis of B. paranthracis revealed 16 CAZyme gene clusters associated with cellulose, amylase, and pectinase activity. Thus, B. paranthracis is a promising probiotic strain that can produce enzymes with biotechnological applications.

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Section: Effect Of Temperaturesupporting

confidence: 89%

Screening and Production of Industrially Relevant Enzymes by Bacillus paranthracis Strain MHDS3, a Potential Probiotic

Tshisikhawe,

Diale,

Abrahams

et al. 2023

Fermentation

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“…B. tropicus JMT retains several anthrax-like virulence genes and displays different characteristics, such as B. cereus G9241 and B. cereus 03BB87, not the non-pathogenic B. tropicus strain [ 39 , 40 , 41 ]. In previous studies, these atypical B. cereus strains have been widely accepted to overcome host immunity and cause severe symptoms, even death.…”

Section: Discussionmentioning

confidence: 99%

Retrospective Screening of Anthrax-like Disease Induced by Bacillus tropicus str. JMT from Chinese Soft-Shell Turtles in Taiwan

2023

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Bacillus cereus is ubiquitous in the environment and a well-known causative agent of foodborne disease. Surprisingly, more and more emerging strains of atypical B. cereus have been identified and related to severe disease in humans and mammals such as chimpanzees, apes, and bovine. Recently, the atypical B. cereus isolates, which mainly derive from North America and Africa, have drawn great attention due to the potential risk of zoonosis. The cluster of B. cereus carries several anthrax-like virulent genes that are implicated in lethal disease. However, in non-mammals, the distribution of atypical B. cereus is still unknown. In this study, we conducted a retrospective screening of the 32 isolates of Bacillus spp. from diseased Chinese soft-shelled turtles from 2016 to 2020. To recognize the causative agent, we used various methods, such as sequencing analysis using PCR-amplification of the 16S rRNA gene, multiplex PCR for discriminating, and colony morphology by following previous studies. Furthermore, the digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values were calculated, respectively, below the 70 and 96% cutoff to define species boundaries. According to the summarized results, the pathogen is taxonomically classified as Bacillus tropicus str. JMT (previous atypical Bacillus cereus). Subsequently, analyses such as targeting the unique genes using PCR and visual observation of the bacteria under various staining techniques were implemented in our study. Our findings show that all (32/32, 100%) isolates in this retrospective screening share similar phenotypical properties and carry the protective antigen (PA), edema factor (EF), hyaluronic acid (HA), and exopolysaccharide (Bps) genes on their plasmids. In this study, the results indicate that the geographic distribution and host range of B. tropicus were previously underestimated.

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“…Furthermore, the biochemical characterization of the studied pectinase also showed high performance under alkaline, thermophilic, and halophilic conditions. There are several reports on alkaline and thermostable pectinase enzymes from various geographic locations and sources, particularly from agro- and industrial-wastes ( Khatri et al, 2015 ; Oumer and Abate, 2017 ; Yu et al, 2017 ; Thakur et al, 2021 ). Regarding soda lakes, only two occurrences of pectin breakdown have thus far been described, both of which describe unique species of the phylum Bacteroidota belonging to the genera Alkaliflexus and Natronoflexus ( Sorokin et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Discovery of novel carbohydrate degrading enzymes from soda lakes through functional metagenomics

et al. 2022

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Extremophiles provide a one-of-a-kind source of enzymes with properties that allow them to endure the rigorous industrial conversion of lignocellulose biomass into fermentable sugars. However, the fact that most of these organisms fail to grow under typical culture conditions limits the accessibility to these enzymes. In this study, we employed a functional metagenomics approach to identify carbohydrate-degrading enzymes from Ethiopian soda lakes, which are extreme environments harboring a high microbial diversity. Out of 21,000 clones screened for the five carbohydrate hydrolyzing enzymes, 408 clones were found positive. Cellulase and amylase, gave high hit ratio of 1:75 and 1:280, respectively. A total of 378 genes involved in the degradation of complex carbohydrates were identified by combining high-throughput sequencing of 22 selected clones and bioinformatics analysis using a customized workflow. Around 41% of the annotated genes belonged to the Glycoside Hydrolases (GH). Multiple GHs were identified, indicating the potential to discover novel CAZymes useful for the enzymatic degradation of lignocellulose biomass from the Ethiopian soda Lakes. More than 73% of the annotated GH genes were linked to bacterial origins, with Halomonas as the most likely source. Biochemical characterization of the three enzymes from the selected clones (amylase, cellulase, and pectinase) showed that they are active in elevated temperatures, high pH, and high salt concentrations. These properties strongly indicate that the evaluated enzymes have the potential to be used for applications in various industrial processes, particularly in biorefinery for lignocellulose biomass conversion.

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Isolation and Characterization of Alkaline Pectinase Productive Bacillus tropicus from Fruit and Vegetable Waste Dump Soil

Cited by 8 publications

References 40 publications

Screening and Production of Industrially Relevant Enzymes by Bacillus paranthracis Strain MHDS3, a Potential Probiotic

Screening and Production of Industrially Relevant Enzymes by Bacillus paranthracis Strain MHDS3, a Potential Probiotic

Retrospective Screening of Anthrax-like Disease Induced by Bacillus tropicus str. JMT from Chinese Soft-Shell Turtles in Taiwan

Discovery of novel carbohydrate degrading enzymes from soda lakes through functional metagenomics

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