First Report of Agrobacterium rhizogenes-induced Hairy Root Formation in Selaginella bryopteris: a Pteridophyte Recalcitrant to Genetic Transformation

Thidiazuron (TDZ) is an active substituted phenyl urea compound that has found a significant role as a plant growth regulator. The most exciting aspect of its function is that it can mimic auxins and cytokinin but is chemically different from these two. Many theories have been put forward, and experiments performed to understand the mode of action of TDZ in callogenesis. One suggested mechanism presents that it works by inhibiting the cytokinin degrading enzymes that compete with cytokinin for an active site on the enzyme. An example is the TDZ‐induced suppressed expression of gibberellic acid (GA) biosynthesis genes encoding GA3 and GA20 oxidases. This is entailed with a slightly increased expression of GA catabolism genes encoding GA20 oxidase. Similarly, one of the recommendations is that TDZ induces the expression of specific genes and transcription regulatory sequences that are either responsible directly for callus formation or in turn induce other auxins or cytokinin for callogenesis. There is no concise review available that discusses the details of TDZ‐induced callus, specifically and other in vitro cultures in general. This review is an attempt to explore all these pathways and mechanisms involved in callogenesis in plants stimulated by TDZ.

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“…For example, the transformation efficiency of Selaginella bryopteris was enhanced TDZ (2 mg/L) to generate hairy roots. 67…”

Section: The Role Of Thidiazuron In Exploiting Hairy Root Culturesmentioning

confidence: 99%

“…Hairy root cultures can produce certain medicinally and commercially when treated with TDZ. For example, the transformation efficiency of Selaginella bryopteris was enhanced TDZ (2 mg/L) to generate hairy roots 67 …”

Section: Applications Of Thidiazuron In Plant Tissue Culturementioning

confidence: 99%