Lipase Production by Aspergillus niger C by Submerged Fermentation

Lima, Laisy Garcia Ribeiro; Gonçalves, Maria Alice Rezende; Couri, Sônia; Melo, Verônica Ferreira; Sant’Ana, Gizele Cardoso Fontes; Costa, Antônio Carlos Augusto da

doi:10.1590/1678-4324-2019180113

Cited by 25 publications

(18 citation statements)

References 31 publications

(32 reference statements)

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“…They catalyze the hydrolysis of triglycerides to FFA and glycerol at the water–lipid interface. Lipases can also catalyze esterification, interesterification, alcoholysis, acidolysis, and aminolysis reactions [ 107 ]. Lipases are classified into extracellular and intracellular based on the nature of the release.…”

Section: Enzyme Catalystmentioning

confidence: 99%

Biodiesel Production Using Homogeneous, Heterogeneous, and Enzyme Catalysts via Transesterification and Esterification Reactions: a Critical Review

2021

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The excessive utilization of petroleum resources leads to global warming, crude oil price fluctuations, and the fast depletion of petroleum reserves. Biodiesel has gained importance over the last few years as a clean, sustainable, and renewable energy source. This review provides knowledge of biodiesel production via transesterification/esterification using different catalysts, their prospects, and their challenges. The intensive research on homogeneous chemical catalysts points to the challenges in using high free fatty acids containing oils, such as waste cooking oils and animal fats. The problems faced are soap formation and the difficulty in product separation. On the other hand, heterogeneous catalysts are more preferable in biodiesel synthesis due to their ease of separation and reusability. However, in-depth studies show the limited activity and selectivity issues. Using biomass waste-based catalysts can reduce the biodiesel production cost as the materials are readily available and cheap. The use of an enzymatic approach has gained precedence in recent times. Additionally, immobilization of these enzymes has also improved the statistics because of their excellent functional properties like easy separation and reusability. However, free/liquid lipases are also growing faster due to better mass transfer with reactants. Biocatalysts are exceptional in good selectivity and mild operational conditions, but attractive features are veiled with the operational costs. Nanocatalysts play a vital role in heterogeneous catalysis and lipase immobilization due to their excellent selectivity, reactivity, faster reaction rates owing to their higher surface area, and easy recovery from the products and reuse for several cycles.

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Section: Enzyme Catalystmentioning

confidence: 99%

Biodiesel Production Using Homogeneous, Heterogeneous, and Enzyme Catalysts via Transesterification and Esterification Reactions: a Critical Review

2021

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“…Submerged fermentation (SmF) are widely used in large-scale microbial lipid production facilities ( Liu et al, 2019 ) and is performed with soluble nutrients submerged in liquid and typically involves three key steps, i.e., fermentation, cell separation, oil extraction and refining ( Čertík et al, 2012 ; Lima et al, 2019 ). SmF offers several advantages, including (i) better monitoring of process parameters, (ii) a homogeneous medium which allows for efficient heat and mass transfer, and (iii) the fungal inoculum remains submerged and distributed evenly, thus favoring nutrient absorption ( Lima et al, 2019 ). Another advantage of SmF is that it facilitates product recovery through simple filtration or centrifugation.…”

Section: Consolidating Processing Units For Efficient Production Of Smentioning

confidence: 99%

The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production

et al. 2021

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Microbial lipids, also known as single-cell oils (SCOs), are highly attractive feedstocks for biodiesel production due to their fast production rates, minimal labor requirements, independence from seasonal and climatic changes, and ease of scale-up for industrial processing. Among the SCO producers, the less explored filamentous fungi (molds) exhibit desirable features such as a repertoire of hydrolyzing enzymes and a unique pellet morphology that facilitates downstream harvesting. Although several oleaginous filamentous fungi have been identified and explored for SCO production, high production costs and technical difficulties still make the process less attractive compared to conventional lipid sources for biodiesel production. This review aims to highlight the ability of filamentous fungi to hydrolyze various organic wastes for SCO production and explore current strategies to enhance the efficiency and cost-effectiveness of the SCO production and recovery process. The review also highlights the mechanisms and components governing lipogenic pathways, which can inform the rational designs of processing conditions and metabolic engineering efforts for increasing the quality and accumulation of lipids in filamentous fungi. Furthermore, we describe other process integration strategies such as the co-production with hydrogen using advanced fermentation processes as a step toward a biorefinery process. These innovative approaches allow for integrating upstream and downstream processing units, thus resulting in an efficient and cost-effective method of simultaneous SCO production and utilization for biodiesel production.

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“…The sterile media in Erlenmeyer flasks were inoculated with spore or hypha suspension until final concentration reached 10 6 CFU/ml [17]. Each isolate inoculated into 4 flasks.…”

Section: Screening Of Lipase-producing Filamentous Fungimentioning

confidence: 99%

Lipase-producing Filamentous Fungi from Non-dairy Creamer Industrial Waste

Triyaswati¹,

Ilmi²

2020

Microbiology and Biotechnology Letters

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Lipase-producing fungi have been isolated from environments containing lipids. The non-dairy creamer industrial waste has a high amount of lipids so it is a potential source for the isolation of lipase-producing fungi. However, the study of fungi that secrete lipase from this industrial waste has not been reported. The purpose of this study was to obtain lipase-producing filamentous fungi from non-dairy creamer industrial waste. Mineral salt and potato dextrose agar were used as media for the isolation process. The qualitative screening was conducted using phenol red agar medium and the quantitative screening using broth medium containing glucose and olive oil. Isolates producing the highest amounts of lipase were identified with molecular methods. We found that 5 out of 19 isolated filamentous fungi are lipase producers. Further analysis showed that isolate Ms.11 produced the highest amount of lipase compared to others. Based on ITS sequence Ms.11 was identified as Aspergillus aculeatus. The lipase activity in medium containing 1% glucose + 1% olive oil at pH 7.0 and 30℃ after 96 and 120 h of incubation was 5.13 ± 0.30 U/ml and 5.22 ± 0.59 U/ml, respectively. The optimum lipase activity was found at pH 7.0, 30℃ and using methanol or ethanol in the reaction tube. Lipase was more stable at 20−30℃ and maintained 85% of its activity. It was concluded that isolate Ms.11 is a potential source of lipase that catalyzes transesterification reactions. Further studies are required to optimize lipase production to make the strain suitable for industry purposes.

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Lipase Production by Aspergillus niger C by Submerged Fermentation

Cited by 25 publications

References 31 publications

Biodiesel Production Using Homogeneous, Heterogeneous, and Enzyme Catalysts via Transesterification and Esterification Reactions: a Critical Review

Biodiesel Production Using Homogeneous, Heterogeneous, and Enzyme Catalysts via Transesterification and Esterification Reactions: a Critical Review

The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production

Lipase-producing Filamentous Fungi from Non-dairy Creamer Industrial Waste

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