Detection of Diarrheagenic Escherichia coli in Bovine Meat in the Northern Region of Paraná State, Brazil

Pelayo, Jacinta Sánchez; Elias, A; Lima, Nicole Ribeiro de; Navarro, Armando; Rocha, Sérgio Paulo Dejato da

doi:10.1590/1678-4324-2019180012

Cited by 3 publications

(1 citation statement)

References 41 publications

(43 reference statements)

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“…[7] In bovine ground meat from Brazil, E. coli O93 was not only identified as STEC but also as an enteroaggregative E. coli (EAEC) pathotype. [8] Furthermore, bacterial E. coli isolates from cattle in Australia revealed that serogroup O93 was common and it was shown to have an antibiotic resistance phenotype, though the specific antibiotics to which the E. coli O93 bacteria were resistant did vary. [9] Avian pathogenic E. coli (APEC) were isolated from ducks in China during an eight-year period, where E. coli O93 was the predominant serogroup, [10] whereas in ducks from Vietnam it was the least common of the isolated serogroups.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of the O-antigen structure of Escherichia coli O93 and biosynthetic aspects thereof

Furevi

Ståhle

Muheim

et al. 2022

Preprint

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The structure of the O-antigen from the international reference strain Escherichia coli O93:−:H16 has been determined. A nonrandom modal chain-length distribution was observed for the lipopolysaccharide, a pattern which is typical when long O-specific polysaccharides are expressed. By a combination of (i) bioinformatics information on the gene cluster related to O-antigen synthesis including putative function on glycosyl transferases, (ii) the magnitude of NMR coupling constants of anomeric protons and (iii) unassigned 2D 1H,13C-HSQC and 1H,1H-TOCSY NMR spectra it was possible to efficiently elucidate the structure of the carbohydrate polymer in an automated fashion using the computer program CASPER. The polysaccharide also carries O-acetyl groups and their locations were determined by 2D NMR experiments showing that ~½ of the population was 2,6-di-O-acetylated, ~¼ was 2-O-acetylated, whereas ~¼ did not carry O-acetyl groups at one of the mannosyl residues. The structure of the tetrasaccharide repeating unit of the O-antigen is given by: →2)-b-D-Manp-(1→3)-b-D-Manp2Ac6Ac-(1→4)-b-D-GlcpA-(1→3)-a-D-GlcpNAc-(1→, which should also be the biological repeating unit and it shares structural elements with capsular polysaccharides from E. coli K84 and K50. The structure of the acidic O-specific polysaccharide from Cellulophaga baltica strain NN015840T differs to that of the O-antigen from E. coli O93 by lacking the O-acetyl group at O6 of the O-acetylated mannosyl residue.

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Section: Introductionmentioning

confidence: 99%

Elucidation of the O-antigen structure of Escherichia coli O93 and biosynthetic aspects thereof

Furevi

Ståhle

Muheim

et al. 2022

Preprint

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Identification of a hybrid atypical enteropathogenic and enteroaggregative Escherichia coli (aEPEC/EAEC) clone of serotype O3:H2 associated with a diarrheal outbreak in Brazil

et al. 2021

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Elucidation of the O-antigen structure of Escherichia coli O93 and characterization of its biosynthetic genes

et al. 2022

View full text Add to dashboard Cite

The structure of the O-antigen from the international reference strain Escherichia coli O93:−:H16 has been determined. A nonrandom modal chain-length distribution was observed for the lipopolysaccharide, a pattern which is typical when long O-specific polysaccharides are expressed. By a combination of (i) bioinformatics information on the gene cluster related to O-antigen synthesis including putative function on glycosyl transferases, (ii) the magnitude of NMR coupling constants of anomeric protons and (iii) unassigned 2D 1H,13C-HSQC and 1H,1H-TOCSY NMR spectra it was possible to efficiently elucidate the structure of the carbohydrate polymer in an automated fashion using the computer program CASPER. The polysaccharide also carries O-acetyl groups and their locations were determined by 2D NMR experiments showing that ~½ of the population was 2,6-di-O-acetylated, ~¼ was 2-O-acetylated, whereas ~¼ did not carry O-acetyl group(s) in the 3-O-substituted mannosyl residue of the repeating unit. The structure of the tetrasaccharide repeating unit of the O-antigen is given by: →2)-β-d-Manp-(1 → 3)-β-d-Manp2Ac6Ac-(1 → 4)-β-d-GlcpA-(1 → 3)-α-d-GlcpNAc-(1→, which should also be the biological repeating unit and it shares structural elements with capsular polysaccharides from E. coli K84 and K50. The structure of the acidic O-specific polysaccharide from Cellulophaga baltica strain NN015840T differs to that of the O-antigen from E. coli O93 by lacking the O-acetyl group at O6 of the O-acetylated mannosyl residue.

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Detection of Diarrheagenic Escherichia coli in Bovine Meat in the Northern Region of Paraná State, Brazil

Cited by 3 publications

References 41 publications

Elucidation of the O-antigen structure of Escherichia coli O93 and biosynthetic aspects thereof

Elucidation of the O-antigen structure of Escherichia coli O93 and biosynthetic aspects thereof

Identification of a hybrid atypical enteropathogenic and enteroaggregative Escherichia coli (aEPEC/EAEC) clone of serotype O3:H2 associated with a diarrheal outbreak in Brazil

Elucidation of the O-antigen structure of Escherichia coli O93 and characterization of its biosynthetic genes

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