Effect of Salicylic Acid, 2,4-D and 2i-P on the Production of Secondary Metabolites in Garcinia brasiliensis Mart. Callus

Teixeira, Maria Gessi; Carvalho, Marília; Leite, Marco Aurélio; Barbosa, Sandro; Filho, Plínio Rodrigues dos Santos; Santos, Breno Régis

doi:10.1590/1678-4324-2019170303

Cited by 3 publications

(1 citation statement)

References 43 publications

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“…However, according to [26] the highest level of phenolic compounds was found in the calluses of Leucojum aestivum initiated and multiplied on the medium supplemented with dicamba. According to [35] Phenols are secondary metabolic compounds present in vegetables and synthesized in the shikimic acid pathway. These compound present different chemical structures as well as different biological properties and can influence the texture of cellular structures.…”

Section: Discussionmentioning

confidence: 99%

Effect of auxin type on the total phenols synthesis and some enzymatic proteins activity in maize callus from seeds irradiated to gamma radiation

Ahou-Nadia¹,

Koutoua²,

Salomé³

et al. 2020

World J. Adv. Res. Rev.

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Secondary metabolites of plants widely used in the medicinal and cosmetic industries, can be produced by tissue culture. In vitro production of these secondary metabolites is affected by various factors such as the type and concentration of phyto regulators. In this study, 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-methoxy-3,6-dichlorobenzoic acid (Dicamba) effect on phenolic compounds synthesis as well as Phenylalanine ammonia lyase (PAL), tyrosine ammonia lyase (TAL), Polyphenoloxidase (PPO), peroxidase (POD), Catalase (CAT) and Ascorbate Peroxidase (APX) activities from root explants have been studied. The root explants come from the seeds in vitro germination of maize irradiated (200 and 300 grays) or not irradiated (control) with gamma radiation. The results obtained showed that the auxin type had a very highly significant effect (p <0.001) on all parameters studied. Callus induced on medium containing 2,4-D gave a higher content of phenolic compounds, PAL, TAL, PPO, POD, CAT and APX than callus induced on medium containing dicamba. The highest content of total phenols (76.971 µmol/g fresh callus) was obtained in callus from 300 grays. By cons, the contents of POD, PAL, TAL, CAT and APX (4.034; 0.031; 0.145; 1315.596 and 2461.714 mmol/min/g fresh callus) were obtained in callus from 200 grays.

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Section: Discussionmentioning

confidence: 99%

Effect of auxin type on the total phenols synthesis and some enzymatic proteins activity in maize callus from seeds irradiated to gamma radiation

Ahou-Nadia¹,

Koutoua²,

Salomé³

et al. 2020

World J. Adv. Res. Rev.

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In Vitro Production of Bioactive Compounds from Plant Cell Culture

Lakshmaiah¹,

Banadka²,

Thirumoorthy³

et al. 2022

Nutraceuticals Production From Plant Cell Factory

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Influence of 2iP and 2,4-D Concentrations on Accumulation of Biomass, Phenolics, Flavonoids and Radical Scavenging Activity in Date Palm (Phoenix dactylifera L.) Cell Suspension Culture

Al-Khayri

Naik

2022

Horticulturae

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Plant hormones are chemical compounds that serve as crucial signal molecules. The growth and induction of bioactive compounds in plant suspension cultures depend on the exogenous application of auxins and cytokinins at different concentrations. In this study, date palm (Phoenix dactylifera L., cv. Shishi) cell suspension cultures initiated in Murashige and Skoog (MS) medium were treated with 2.5 and 5 mg/L 2-isopentenyladenine (2-iP) in combinations with 1, 2.5, 5 and 10 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), and compared to the standard cell multiplication medium as the control, which contained 1.5 mg/L 2iP and 10 mg/L naphthaleneacetic acid (NAA). The optimum biomass accumulation, based on the packed cell volume and dry weight, was found in the cell suspension culture augmented with 1 mg/L 2,4-D + 5 mg/L 2iP followed by 5 mg/L 2,4-D + 2.5 mg/L 2-iP. In addition, the study assessed the total phenolic, flavonoid and radical scavenging activity, and high performance liquid chromatography (HPLC) was also used to determine the content of polyphenols (apigenin, caffeic acid, catechin and kaempferol) quantitatively. Cell suspension cultures containing 5 mg/L 2,4-D and 2.5 mg/L 2iP yielded the maximum accumulation of phenolics, flavonoids and radical scavenging activity (90.65%), and also a significantly higher content of caffeic acid (37.1 µg/g DW). The present finding facilitates the scaling up and commercial production of polyphenols from date palm cell suspension culture.

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Effect of Salicylic Acid, 2,4-D and 2i-P on the Production of Secondary Metabolites in Garcinia brasiliensis Mart. Callus

Cited by 3 publications

References 43 publications

Effect of auxin type on the total phenols synthesis and some enzymatic proteins activity in maize callus from seeds irradiated to gamma radiation

Effect of auxin type on the total phenols synthesis and some enzymatic proteins activity in maize callus from seeds irradiated to gamma radiation

In Vitro Production of Bioactive Compounds from Plant Cell Culture

Influence of 2iP and 2,4-D Concentrations on Accumulation of Biomass, Phenolics, Flavonoids and Radical Scavenging Activity in Date Palm (Phoenix dactylifera L.) Cell Suspension Culture

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