Hydrogen Peroxide Induced Oxidative Damage on Mineral Density and Mechanical Properties of Bone

Çiçek, Ekrem; Çakmak, Enes

doi:10.1590/1678-4324-2018180043

Cited by 4 publications

(5 citation statements)

References 31 publications

(37 reference statements)

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“…The samples were placed in the designed tester device, and the resulting diagrams were obtained for the LR5K Plus tester [14]. The test results were drawn as diagrams in NEXYGEN Plus Data Analysis Software and MS Excel 2010.…”

Section: Compression Tests For Instantaneous Modulusmentioning

confidence: 99%

Effects of radiopharmaceuticals on articular cartilage’s mechanical properties

Kuzu

Çiçek

2019

Nukleonika

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As radiation science and technology advances, nuclear medicine applications are increasing worldwide which necessitate the understanding of biological implications of such practices. Ionizing radiation has been shown to cause degraded matrix and reduced proteoglycan synthesis in cartilage, and the late consequences of which may include degenerative arthritis or arthropathy. Although degenerative effects of the ionizing radiation on cartilage tissue have been demonstrated, the effects on the mechanical properties of articular cartilage are largely unknown. The radiopharmaceuticals, technetium-99m and technetium-99m sestamibi, were utilized on bovine articular cartilage to investigate these effects. We used two different mechanical tests to determine the mechanical properties of articular cartilage. Dynamic and static mechanical tests were applied to calculate compressive modulus for articular cartilage. We observed clearly higher control modulus values than that of experimental groups which account for lesser stiffness in the exposed cartilage. In conclusion, compressive moduli of bovine articular cartilage were found to decrease after radiopharmaceutical exposure, after both instantaneous and equilibrium mechanical experiments.

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Section: Compression Tests For Instantaneous Modulusmentioning

confidence: 99%

Effects of radiopharmaceuticals on articular cartilage’s mechanical properties

Kuzu

Çiçek

2019

Nukleonika

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“…Also, the biogenesis of the bone tissue is influenced by other factors, such as the overproduction of reactive oxygen species (ROS) induced by aging or diseases like cancer or other chronic diseases. Hydrogen peroxide (H 2 O 2 ) is a type of ROS that potentially inflicts harm to the bone (9)(10)(11) and is associated with the increased formation and function of osteoclasts (12). Oxidative stress may contribute to bone loss under normal physiological or pathological conditions by reducing the production of bone growth factors.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Aerobic Training and Cholecalciferol on IGF-I and IGFBP-3 in the Bone Tissue of Rats Poisoned with Hydrogen Peroxide

Eskandari

Matinhomaee

Moradi

2022

Jentashapir J Cell Mol Biol

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Background: Exercise and vitamin D can improve bone density by reducing bone loss. Growth factors such as IGF-I and IGFBP-3 are appeared to increase bone turnover in response to mechanical load, and free radicals attenuate the release of these growth factors. Objectives: We assessed the effect of concurrent aerobic training and cholecalciferol administration along with hydrogen peroxide injection on the levels of IGF-I and IGFBP-3 expression in the bone tissue of rats. Methods: Thirty-six adult Wistar rats were randomized into six groups (n = 6), including healthy control, sham, hydrogen peroxide (H2O2), H2O2 + aerobic training, H2O2 + cholecalciferol, and H2O2 + aerobic training + cholecalciferol. The rats were intraperitoneally administered with one mmol/kg. Body weight (BW) of H2O2 three times a week on even days and 0.5 μg/kg.bw of cholecalciferol daily. Aerobic training (at a speed of 4 - 20 m/min, for 20 - 60 minutes) was performed five days/w for eight weeks. The expression of IGF-I and IGFBP-3 was measured by real-time (RT)-PCR. Data were analyzed using the independent t-test, two-way ANOVA (exercise × vitamin D), and Bonferroni’s post-hoc test in SPSS 26 at the significance level of P ≤ 0.05. Results: The results showed that H2O2 significantly reduced the gene expressions of IGF-I (P = 0.001) and IGFBP-3 (P = 0.001) in the bone tissue. Also, exercise and vitamin D augmented the expression of IGF-I (P = 0.008) and IGFBP-3 (P = 0.0001) as post-hoc analysis showed that aerobic training had the greatest effect on the expression of IGF-I and IGFBP-3 (P < 0.05). In addition, the amplifying effects of aerobic training and cholecalciferol on the gene expressions of IGF-I and IGFBP-3 were also remarkable (P < 0.1). Conclusions: The mechanical load created by aerobic training exerted the greatest augmenting effect on the gene expression of IGF-I and IGFBP-3. Moreover, the interactive effect of aerobic training and cholecalciferol was also significant.

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Section: Introductionmentioning

confidence: 99%

“…Bone is a dynamic tissue whose homeostasis is regulated by the balance between osteoblasts (bone formation) and osteoclasts (bone breakdown) (1). However, the increase in Reactive Oxygen Species (ROS) is one of the factors affecting bone health (1,2). Increasing ROS causes mitochondrial damage and reduces bone mineralization by binding to membrane proteins, cytosolic proteins, and even DNA in the bone, leading to osteoporosis and fractures (1).…”

Section: Introductionmentioning

confidence: 99%

“…However, the increase in Reactive Oxygen Species (ROS) is one of the factors affecting bone health (1,2). Increasing ROS causes mitochondrial damage and reduces bone mineralization by binding to membrane proteins, cytosolic proteins, and even DNA in the bone, leading to osteoporosis and fractures (1). Increasing ROS can also lead to the activation of receptor activator of nuclear factor κB ligand (RANKL).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Aerobic Training and Vitamin D Consumption on NFATc1 Gene Expression in Bone Tissue of Rats Exposed to H2O2

2020

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Background: Increased reactive oxygen species disrupt the balance between osteoblasts and osteoclasts. Although the role of training (T) and vitamin D (VD) consumption in bone health has been shown, there is no accurate information on the role of these two interventions on the nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), as an osteoclast marker. Objectives: Therefore, this study aimed to examine the effect of T and VD on NFATc1 gene expression in bone tissue of rats exposed to H2O2. Methods: Fifty adult male Wistar rats aged 8 - 10 weeks and weighing 180 to 220 g were randomly assigned to 10 groups including (1) control (C), (2) dimethyl sulfoxide + normal saline (sham; Sh), (3) 1 mmol/kg H2O2 (1H), (4) 1H+VD, (5) 1H+T, (6) 1H+VD+T, (7) 2 mmol/kg H2O2 (2H), (8) 2H+VD, (9) 2H+T, and (10) 2H+VD+T. The research protocol lasted eight weeks to implement. The levels of NFATc1 gene expression were measured by qRT-PCR. Results: Based on the results, 1H and 2H significantly increased NFATc1 gene expression levels (P = 0.001). However, T (P = 0.001), VD (P = 0.001), and VD+T (P = 0.001) reduced NFATc1 gene expression in the bone tissue of rats exposed to 1 and 2 mmol H2O2. Also, NFATc1 gene expression was significantly lower in the 1H+VD+T group than in the 2H + VD group (P = 0.03). Conclusions: It seems that T and VD consumption both alone and synergistically have a reducing effect on NFATc1 as an osteoclast index in rats exposed to 1 and 2 mmol/kg H2O2.

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Hydrogen Peroxide Induced Oxidative Damage on Mineral Density and Mechanical Properties of Bone

Cited by 4 publications

References 31 publications

Effects of radiopharmaceuticals on articular cartilage’s mechanical properties

Effects of radiopharmaceuticals on articular cartilage’s mechanical properties

The Effect of Aerobic Training and Cholecalciferol on IGF-I and IGFBP-3 in the Bone Tissue of Rats Poisoned with Hydrogen Peroxide

Effect of Aerobic Training and Vitamin D Consumption on NFATc1 Gene Expression in Bone Tissue of Rats Exposed to H2O2

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