Relationship Between the Anxious Symptoms and the Neurotransmitter in Parkinson's Mice with Different Dosages of MPTP

Abstract

This study aims to explore the relationship between the anxious symptoms and the impairment of 5hydroxytryptamine system in PD mice induced by different dosages of MPTP. The mice from the three model groups, the low-dose, dose and high-dose group, took longer time in the dark box than those in the control group (P<0.05). However, no statistically significant differences were found among the model groups. The number of open arm entry (OE) and the open arm time (OT) were significant lower in the model group than those in the control group in the elevated plus-maze test (P＜0.05). The percentage of OE in modle group was significantly lower compared with the control group (P＜0.05). The concentrations of striatum DA, HVA, 5-HT, and 5-HIAA were significantly reduced in the three model groups compared to the control group (P＜0.05). The 5-HT concentrations of high-dose group was significantly lower than those of the control group in the prefrontal cortex (P＜0.05). Anxiety symptoms were appeared in the three model groups of early PD mice, but no difference existed among these groups. The 5-hydroxytryptamine system was damaged after MPTP injection, which could lead to anxiety. However, the impairment of 5-hydroxytryptamine system induced by MPTP was dose-independent.

Glucagon‐like peptide‐1 analog improves neuronal and behavioral impairment and promotes neuroprotection in a rat model of aluminum‐induced dementia

“…Solid phase sandwich ELISA (AbC 606 and AbC 607, respectively; Votrefournisseur AbCysS.A. Paris, France) measured the IFN‐γ concentrations and represented them as pg/mg. Measurement of malondialdehyde (MDA) and superoxide dismutase (SOD):Colormetric kits supported by Biodiagnostic co (Biodiagnostic, Giza, Egypt) were approximated by MDA and SOD and expressed as nmol/ mg and U/ mg, respectively (Xia et al., 2018). Measurement of hippocampal tissue homogenate adrenaline, noradrenaline, and dopamine:The concentrations of adrenaline, noradrenaline, and dopamine of the samples were analyzed with HPLC (Agilent Technologies 1,200 Series, G1315D DAD) according to the method of Zagrodzka et al (Zagrodzka et al., 2000).…”

“…Colormetric kits supported by Biodiagnostic co (Biodiagnostic, Giza, Egypt) were approximated by MDA and SOD and expressed as nmol/ mg and U/ mg, respectively (Xia et al., 2018).…”