Escherichia Coli: Characteristics of Carbapenem Resistance and Virulence Factors

Candan, Esra Deniz; Aksöz, Nilüfer

doi:10.1590/1678-4324-2017160416

Cited by 13 publications

(9 citation statements)

References 38 publications

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“…Among MDR E. coli, AMR caused by ESBL is mainly due to the bla CTX-M family, particularly bla CTX-M-15 and -14 , compared to the less frequently observed bla SHV and bla OXA families [6][7][8]. As per the literature, carbapenem resistance in E. coli is mostly mediated by bla OXA-48 [9], bla NDM and bla VIM genes [10]. Also, increasingly, resistance is being reported for fluoroquinolones and third-and fourth-generation cephalosporins and ST-131 predominates globally among such MDR E. coli strains [11].…”

Section: Introductionmentioning

confidence: 79%

“…Globally, bla OXA-48 type were the most commonly reported carbapenemases among E. coli [21], followed by bla NDM [22], bla IMP [23] and bla KPC [24]. Studies have reported occurrence of bla OXA-48 from as low as 3% to 22% [25,9]. In contrast, a previous report from India on carbapenem-resistant clinical E. coli isolates from 2013 and 2015 has shown that bla NDM was common among carbapenemases in E. coli (70%), followed by bla OXA-48 (24%) and bla VIM (17%).…”

Section: Discussionmentioning

confidence: 99%

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First Indian report on genome-wide comparison of multidrug-resistant Escherichia coli from blood stream infections

et al. 2020

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BackgroundMultidrug-resistant (MDR) E. coli with extended-spectrum β-lactamases (ESBLs) is becoming endemic in health care settings around the world. Baseline data on virulence and antimicrobial resistance (AMR) of specific lineages of E. coli circulating in developing countries like India is currently lacking. MethodsWhole-genome sequencing was performed for 60 MDR E. coli isolates. The analysis was performed at single nucleotide polymorphism (SNP) level resolution to identify the presence of their virulence and AMR genes. ResultsGenome comparison revealed the presence of ST-131 global MDR and ST410 as emerging-MDR clades of E. coli in India. AMR gene profile for cephalosporin and carbapenem resistance differed between the clades. Genotypes bla CTX-M-15 and bla NDM-5 were common among cephalosporinases and carbapenemases, respectively. For aminoglycoside resistance, rmtB was positive for 31.7% of the isolates, of which 95% were co-harboring carbapenemases. In addition, the FimH types and virulence gene profile positively correlated with the SNP based phylogeny, and also revealed the evolution of MDR clones among the study population with temporal accumulation of SNPs. The predominant clone was ST167 (bla NDM lineage) followed by ST405 (global clone ST131 equivalent) and ST410 (fast spreading high risk clone).

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Section: Introductionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

First Indian report on genome-wide comparison of multidrug-resistant Escherichia coli from blood stream infections

et al. 2020

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“…bla OXA-48 type carbapenemases are the most commonly reported in E. coli [19] followed by bla NDM [20], bla IMP [21] and bla KPC [22] type carbapenemases have also been determined. Studies report occurrence of bla OXA-48 from as low as 3% to 22% [23, 9]. A report from India has shown, bla NDM was common among carbapenemases in E. coli (70%), followed by bla OXA-48 (24%) and bla VIM (17%).…”

Section: Discussionmentioning

confidence: 99%

“…Among MDR E. coli , AMR mediated by ESBL is mainly due to the bla CTX-M family, particularly bla CTX-M-15 and 14, compared to the less frequent observation of bla SHV and bla OXA families [6–8]. Carbapenem resistance in E. coli was mostly reported to be mediated by bla OXA-48 [9], bla NDM and bla VIM [10]. Also, AMR among E. coli is increasingly reported for fluoroquinolones and third- and fourth-generation cephalosporins.…”

Section: Introductionmentioning

confidence: 99%

First Indian report on Genome-wide Comparison of Multidrug-ResistantEscherichia colifrom Blood Stream Infections

Ragupathi

Veeraraghavan

Sethuvel

et al. 2019

Preprint

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BackgroundMultidrug-resistant (MDR)E. coliwith extended-spectrum β-lactamases (ESBLs) is becoming endemic in health care settings around the world. Baseline data on virulence and AMR of specific lineages ofE. colicirculating in developing countries like India is currently lacking.MethodsWhole-genome sequencing was performed for 60 MDRE. coliisolates. Genome-wide analysis was performed at single nucleotide polymorphism (SNP) level resolution to identify the relation between the isolates in context of time, virulence and AMR determinants possessed.ResultsGenome comparison revealed the presence of ST-131 global MDR and ST410 as emerging-MDR clades ofE. coli. AMR gene profile for cephalosporin and carbapenem resistance differed between the clades. GenotypesblaCTX-M-15andblaNDM-5were common among cephalosporinases and carbapenemases, respectively. For aminoglycoside resistance,rmtBwas positive for 31.7% of the isolates, of which 30% were co-harboring carbapenemases. Further, the FimH types and virulence gene profile positively correlated with the SNP based phylogeny, which also revealed the evolution of MDR clones among the study population with temporal accumulation of SNPs. The predominant clone was ST167 (blaNDMlineage) followed by ST405 (global clone ST131 equivalent) and ST410 (fast spreading high risk clone).ConclusionsThis is the first report on the whole genome analysis of MDRE. colilineages circulating in India. Data from this study will provide public health agencies a baseline portfolio of AMR and virulence in pathogenicE. coliin the region.

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“…Escherichia coli is one of the most common causative agents of a wide range of clinical infections varying from meningitis, urinary tract infections, and sepsis. [1] Carbapenems are the only antibiotic option left for the treatment of infections caused by multidrug-resistant E. coli . However, the emergence of carbapenem resistance leads to the failure of empirical therapy with carbapenems.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and characterization of carbapenemase-producing Escherichia coli from a tertiary care hospital in India

Govindaswamy

Bajpai

Khurana

et al. 2019

J Global Infect Dis

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The purpose of this study was to estimate the prevalence and to characterize the carbapenemase-producing Escherichia coli by various phenotypic antimicrobial susceptibility testing methods, and its performance was compared to the gold standard genotypic method. The prevalence of carbapenemase-resistant E. coli was found to be 65%. The phenotypic methods evaluated are cost-effective and can be used in resource-limited laboratories to rule out carbapenem resistance.

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Escherichia Coli: Characteristics of Carbapenem Resistance and Virulence Factors

Cited by 13 publications

References 38 publications

First Indian report on genome-wide comparison of multidrug-resistant Escherichia coli from blood stream infections

First Indian report on genome-wide comparison of multidrug-resistant Escherichia coli from blood stream infections

First Indian report on Genome-wide Comparison of Multidrug-ResistantEscherichia colifrom Blood Stream Infections

Prevalence and characterization of carbapenemase-producing Escherichia coli from a tertiary care hospital in India

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