Endophytic Microbiota Associated with the Root Tips and Leaves of Baccharis dracunculifolia

Santana, Raiana Silva Menezes de; Fernandes, Geraldo Wilson; Ávila, Marcelo P.; Reis, Mariana P.; Araújo, Flávio Marcos Gomes; Salim, Anna C. M.; Oliveira, Guilherme; Chartone-Souza, Edmar; Nascimento, Andréa M. A.

doi:10.1590/1678-4324-2016160287

Cited by 8 publications

(6 citation statements)

References 65 publications

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“…The highest evenness of bacterial communities was found in the stem among plant parts. It should be noticed that there was no significant difference in bacterial diversity between stems and roots, while many studies have determined that bacterial diversity was highest in roots (Santana et al, 2016;Wang et al, 2019). The variation of endophytic bacterial communities in different compartments was primarily driven by tissue-specific filtering mechanisms within the host (Bonito et al, 2014;Chao et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

The Macleaya cordata Symbiont: Revealing the Effects of Plant Niches and Alkaloids on the Bacterial Community

et al. 2021

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Endophytes are highly associated with plant growth and health. Exploring the variation of bacterial communities in different plant niches is essential for understanding microbe-plant interactions. In this study, high-throughput gene sequencing was used to analyze the composition and abundance of bacteria from the rhizospheric soil and different parts of the Macleaya cordata. The results indicated that the bacterial community structure varied widely among compartments. Bacterial diversity was observed to be the highest in the rhizospheric soil and the lowest in fruits. Proteobacteria, Actinobacteria, and Bacteroidetes were found as the dominant phyla. The genera Sphingomonas (∼47.77%) and Methylobacterium (∼45.25%) dominated in fruits and leaves, respectively. High-performance liquid chromatography (HPLC) was employed to measure the alkaloid content of different plant parts. Significant correlations were observed between endophytic bacteria and alkaloids. Especially, Sphingomonas showed a significant positive correlation with sanguinarine and chelerythrine. All four alkaloids were negatively correlated with the microbiota of stems. The predicted result of PICRUST2 revealed that the synthesis of plant alkaloids might lead to a higher abundance of endophytic microorganisms with genes related to alkaloid synthesis, further demonstrated the correlation between bacterial communities and alkaloids. This study provided the first insight into the bacterial community composition in different parts of Macleaya cordata and the correlation between the endophytic bacteria and alkaloids.

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Section: Discussionmentioning

confidence: 99%

The Macleaya cordata Symbiont: Revealing the Effects of Plant Niches and Alkaloids on the Bacterial Community

et al. 2021

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“…Interestingly, a distinctive pattern in the phyllosphere diazotrophic community composition seen in this study is the fact that the genus Rhodoplanes (59.58% on average) belonging to Alphaproteobacteria dominates the assemblage on IP phyllosphere sampled here, while a very low abundance of Rhodoplanes found in other phyllosphere samples. Whereas members of this group are generally represented in the communities of roots, compared with leaves [64]. Representatives of Rhodoplanes have been isolated from pond water [65] and rhizosphere soil, and are characterized by their capacity for growing phototrophically and chemotrophically [66].…”

Section: Discussionmentioning

confidence: 99%

Distinct Microbial Community of Phyllosphere Associated with Five Tropical Plants on Yongxing Island, South China Sea

Bao

Cai

Zhang³

et al. 2019

Microorganisms

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The surfaces of a leaf are unique and wide habitats for a microbial community. These microorganisms play a key role in plant growth and adaptation to adverse conditions, such as producing growth factors to promote plant growth and inhibiting pathogens to protect host plants. The composition of microbial communities very greatly amongst different plant species, yet there is little data on the composition of the microbiome of the host plants on the coral island in the South China Sea. In this study, we investigated the abundances and members of a major microbial community (fungi, bacteria, and diazotrophs) on the leaves of five dominant plant species (Ipomoea pes-caprae, Wedelia chinensis, Scaevola sericea, Cocos nucifera, and Sesuvium portulacastrum) on the island using real-time quantitative polymerase chain reaction (PCR) and high-throughput amplicon sequencing. Quantitative PCR results showed that fungi and bacteria were ubiquitous and variable among different host plants. Scaevola sericea showed the lowest absolute abundance and highest diversity of fungi and bacteria, while Cocos nucifera had the lowest abundance and the highest diversity of diazotrophs compare to the other four plants. There was a small proportion of shared microorganisms among the five different plants, while unique fungi, bacteria and diazotrophs were significantly enriched for different host plant species in this study (p < 0.05). Some of the most abundant organisms found in the communities of these different host plants are involved in important biogeochemical cycles that can benefit their host, including carbon and nitrogen cycles.

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“…In contrast, representatives of Propionibacteriaceae and Solirubrobacteraceae capable of CH 3 Cl degradation have not been described thus far. Friedmaniella members closely related to Propionibacteriaceae have been detected in the phyllosphere of magnolia trees (Jackson and Denney, 2011), hemlock plants (Rogers et al ., 2018), in grasses (Aydogan et al ., 2020), shrubs (Santana et al ., 2016) and hardwood forest trees (Herrmann et al ., 2020). Bacteria of the family Solirubrobacteraceae can be strictly aerobic and chemoorganotrophic (Albuquerque and da Costa, 2014), and have been identified in the phyllosphere of grasses (Aydogan et al ., 2020), shrubs (Santana et al ., 2016) and in olive trees (Fausto et al ., 2018).…”

Section: Resultsmentioning

confidence: 99%

¹³C‐chloromethane incubations provide evidence for novel bacterial chloromethane degraders in a living tree fern

Kröber

Wende

Kanukollu

et al. 2021

Environmental Microbiology

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Chloromethane (CH 3 Cl) is the most abundant halogenated volatile organic compound in the atmosphere and contributes to stratospheric ozone depletion. CH 3 Cl has mainly natural sources such as emissions from vegetation. In particular, ferns have been recognized as strong emitters. Mitigation of CH 3 Cl to the atmosphere by methylotrophic bacteria, a global sink for this compound, is likely underestimated and remains poorly characterized. We identified and characterized CH 3 Cl-degrading bacteria associated with intact and living tree fern plants of the species Cyathea australis by stable isotope probing (SIP) with 13 C-labelled CH 3 Cl combined with metagenomics.Metagenome-assembled genomes (MAGs) related to Methylobacterium and Friedmanniella were identified as being involved in the degradation of CH 3 Cl in the phyllosphere, i.e., the aerial parts of the tree fern, while a MAG related to Sorangium was linked to CH 3 Cl degradation in the fern rhizosphere. The only known metabolic pathway for CH 3 Cl degradation, via a methyltransferase system including the gene cmuA, was not detected in metagenomes or MAGs identified by SIP. Hence, a yet uncharacterized methylotrophic cmuA-independent pathway may drive CH 3 Cl degradation in the investigated tree ferns.

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Endophytic Microbiota Associated with the Root Tips and Leaves of Baccharis dracunculifolia

Abstract: ABSTRACT

Cited by 8 publications

References 65 publications

The Macleaya cordata Symbiont: Revealing the Effects of Plant Niches and Alkaloids on the Bacterial Community

The Macleaya cordata Symbiont: Revealing the Effects of Plant Niches and Alkaloids on the Bacterial Community

Distinct Microbial Community of Phyllosphere Associated with Five Tropical Plants on Yongxing Island, South China Sea

¹³C‐chloromethane incubations provide evidence for novel bacterial chloromethane degraders in a living tree fern

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Endophytic Microbiota Associated with the Root Tips and Leaves of Baccharis dracunculifolia

Abstract: ABSTRACT

Cited by 8 publications

References 65 publications

The Macleaya cordata Symbiont: Revealing the Effects of Plant Niches and Alkaloids on the Bacterial Community

The Macleaya cordata Symbiont: Revealing the Effects of Plant Niches and Alkaloids on the Bacterial Community

Distinct Microbial Community of Phyllosphere Associated with Five Tropical Plants on Yongxing Island, South China Sea

13C‐chloromethane incubations provide evidence for novel bacterial chloromethane degraders in a living tree fern

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¹³C‐chloromethane incubations provide evidence for novel bacterial chloromethane degraders in a living tree fern