Identification and characterization of Fusarium mangiferae as pathogen of mango malformation in India

Kumar, Pradeep; Kamle, Madhu; Misra, A. K.; O’Donovan, Anthonia; Pagano, Marcela C.; Modi, Dinesh Raj

doi:10.1590/1678-4324-2016160280

Cited by 4 publications

(5 citation statements)

References 30 publications

(37 reference statements)

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“…The molecular analysis was performed from DNA extraction, according to the modified methodology described by MURRAY; THOMPSON (1980), followed by the amplification of the regions ITS-5.8SrDNA, for all the fungi isolated and of a part of the gene for β-tubulin, only for the fungi of the genera Alternaria and Fusarium. The ITS-5.8SrDNA region is one of the most used for the initial identification of the fungi, for being highly conserved in fungal species, but variable among species and, for this reason, it has helped in the studies of phylogenetic relationships among species of great economic importance, such as those from the genus Fusarium (O'BRIEN et al, 2005;KVAS et al, 2009;KACHUEI et al, 2015;KUMAR et al, 2016). The gene for β-tubulin codes for the structural proteins of microtubules and other structural components in eukaryotes and has been widely used in fungal phylogenetic analysis, since it contains variable and highly conserved regions, presenting more stability and reliability in the differentiation of species such as those of Fusarium in comparison to the gene ITS (NOSRATABADI et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Pathogenicity and transmission of fungi detected on Passiflora alata seeds

et al. 2018

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Passion fruit is usually propagated by seeds because of the ease and lower cost in seedling production. However, the seed is the most efficient agent for the spread of pathogens. The damages from seed-borne diseases occur mainly during the germination stages or at the formation of seedlings in nurseries. Considering the need for knowledge on the pathology of sweet passion fruit seeds, the objective was to evaluate the transmission and pathogenicity of the fungi Alternaria sp., Botrytis fabae, Cladosporium cladosporioides, Fusarium spp. and Lasiodiplodia theobromae, known as potentially pathogenic to this crop, and isolated from sweet passion fruit seeds. Therefore, tests on seed health, germination and seedling emergence in a sterilized commercial substrate were conducted using seeds from this species, inoculated with those fungal isolates. Leaves, stems and fruit from this plant were also inoculated with the same fungi. Alternaria sp., Fusarium spp. and L. theobromae were identified in seedlings obtained from inoculated seeds, confirming the transmission of these fungi by seeds. L. theobromae was also considered the most harmful fungus to passion fruit crop, as it causes seed rot and other disease symptoms on the leaves, stem and fruit. These findings inferred that healthy seeds of sweet passion fruit are essential for producing seedlings and to prevent the spread of the diseases caused by these fungi to exempt areas.

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Section: Methodsmentioning

confidence: 99%

Pathogenicity and transmission of fungi detected on Passiflora alata seeds

et al. 2018

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“…In addition, UPGMA analysis was necessary to find out the similarity among the different species. Similarly, in another study Kumar et al (2016) also emphasized on the importance of ITS, BLAST and phylogenetic analysis in the identification and characterization of F. mangiferae as pathogen of mango malformation in India.…”

Section: Upgma Analysismentioning

confidence: 96%

Diversity and identity of Fusarium species occurring on fruits, vegetables and food grains

Ingle

2017

Nusantara Biosci

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Abstract. Ingle AP. 2017. Diversity and identity of Fusarium species occurring on fruits, vegetables and food grains. Nusantara Bioscience 9: 44-51. The members of the genus Fusarium are the most important pathogens associated with different plants including crop plants, fruits, vegetables, etc. Fusarium species are mainly responsible for wilts, blights, root-rots and cankers in these plants. Fusarium spp. produces many types of mycotoxins, which can unfavorably affect human and animal health. In the present study, 48 isolates of Fusarium were recovered from various fruits, vegetables and food grains. Out of these, 23 isolates were recovered from vegetables, 14 from food grains and 11 from fruits. Further, different isolates recovered from infected plant materials were identified on the basis of morphological, cultural and microscopic characteristics.

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“…Tef1α gene was amplified under following cycles; initial denaturation at 94 ⁰C for 90 s, 35 cycles of denaturation at 95 ⁰C for 35 s, annealing at 57 ⁰C for 55 s, extension at 72 ⁰C for 90 s and final extension at 72 ⁰C for 10 min. The PCR program of beta-tubulin was held through the following cycles; initial denaturation at 94 ⁰C for 1 min, 35 cycles of denaturation at 94 ⁰C for 35 s, annealing at 58 ⁰C for 2 min, extension at 72 ⁰C for 1 min, final extension at 72 ⁰C for 10 min and soaked at 4 ⁰C (Kumar et al, 2016).…”

Section: Amplification Of Tef1α and β-Tubulin Genesmentioning

confidence: 99%

Morphological, phylogenetic and pathogenicity characterisation of Fusariumspecies associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus)

Aris¹

2020

Asian J Agric Biol

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Fusarium is a well-known soil-borne fungus where most species belonged in this genus is prominently phytopathogenic. Nevertheless, this pathogenic species has affected the production of pumpkin worldwide. This study underlines the morphological, phylogeny and pathogenicity characteristics of Fusarium for a better disease-control strategy. Twenty-six Fusarium isolates were collected from wilt infected pumpkin in various locations of Peninsular Malaysia. From the combinations of morphological and molecular identifications, four species were identified as F. oxysporum (2 isolates), F. solani (4 isolates), F. proliferatum (7 isolates) and F. incarnatum (13 isolates). Microscopic and macroscopic observation visualized distinct characteristics of the identified Fusarium species. Sequence analyses of tef1α and β-tub genes inferred by maximum likelihood tree resulted in distinct section-specific characteristics. Meanwhile, pathogenicity test of Fusarium isolates presented by the seed inoculation produced various degrees of severities. Fusarium solani C2526P recorded the highest severity of 93.8% after 30 days of post inoculation (dpi). Symptoms have been identified as early as 10 dpi producing stunted growth of the plants. On the other hand, Fusarium oxysporum D2532P recorded 85.3% disease severity. Pathogenic Fusarium caused stunted growth, chlorosis, wilting and necrosis especially at the root of pumpkin plants. This study provides valuable information and methods to manage wilt infected pumpkin in the future.

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Identification and characterization of Fusarium mangiferae as pathogen of mango malformation in India

Abstract: ABSTRACT

Cited by 4 publications

References 30 publications

Pathogenicity and transmission of fungi detected on Passiflora alata seeds

Pathogenicity and transmission of fungi detected on Passiflora alata seeds

Diversity and identity of Fusarium species occurring on fruits, vegetables and food grains

Morphological, phylogenetic and pathogenicity characterisation of Fusariumspecies associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus)

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