Characterization of Antimycins – Producing Streptomycete Strain VY46 Isolated from Slovak Soil

Charousová, Ivana; Steinmetz, Heinrich; Medo, Juraj; Javoreková, Soňa; Wink, Joachim

doi:10.1590/1678-4324-2016160274

Cited by 5 publications

(6 citation statements)

References 28 publications

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“…The physiological testing holds three different approaches for the characterization of Actinobacteria strains. The resistance toward sodium chloride test is taken by analyzing the growth on basal medium with 0, 2.5, 5, 7.5, and 10% of sodium chloride [ 23 ]. The utilization of 10 different carbon sources was determined on the basis of the 20 methodology using a microplate technique with twelve well plates.…”

Section: Methodsmentioning

confidence: 99%

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Screening and Characterization of Streptomyces spp. Isolated from Three Moroccan Ecosystems Producing a Potential Inhibitor of the Drug Efflux Pump AcrAB-TolC

Azmani

Lemriss

Barakate

et al. 2022

BioTech

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Traditional antimicrobial antibiotics are increasingly suffering from the emergence of multidrug resistance among pathogenic microorganisms. The antibiotic era is threatened by the ruthless rise of resistance in bacterial infections. A significant role in these resistance profiles is attributed to multidrug efflux pumps. Hence, much effort is being directed towards developing new compounds to overcome this problem. During our screening program of efflux pumps inhibitors (EPI) produced by bioactive Moroccan Actinobacteria, 210 isolates were screened for their antibacterial activities against Escherichia coli strains containing a system of efflux pump AcrAB-TolC, fully functional, and its mutant, inactivated due to the insertion of transposon Tn903 in AcrAB operon, using the method of agar disc diffusion. The results showed that 14 isolates were able to produce EPI as they were active against the wild type strain but not against the mutant in comparison with the synthetic inhibitor L-Phe-L-Arg-β-naphthylamide (PaβN). We focused on the highest EPI activity produced by four strains (Z332, Z35/G, Z385/b and 136). Taxonomic studies and the 16S rDNA sequence indicated that these strains belonged to the Streptomyces species. This work could contribute to the discovery of a new class of antibacterial agents that could expand the therapeutic arsenal.

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Section: Methodsmentioning

confidence: 99%

“…Commercially available test kits such as ApiZym ® (bioMérieux, Marcy-l’Étoile, France) were used for the biochemical characteristics of the strains. The Api stripes were inoculated following the manufacturer’s manual directions [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Screening and Characterization of Streptomyces spp. Isolated from Three Moroccan Ecosystems Producing a Potential Inhibitor of the Drug Efflux Pump AcrAB-TolC

Azmani

Lemriss

Barakate

et al. 2022

BioTech

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“…Fifty bacterial strains out of 137 were selected on the basis of color and morphological appearance, from which 24 were sequenced and their respective 16S rDNA sequences were identified by BLAST (BLASTN 2.8.1+) [46]. Cultures were maintained in marine broth, and pre-cultures were grown on medium 5294 [47] in a rotatory shaker incubator (50 mL, 30 °C, XAD resin, 3 days). Precultures were in duplicate.…”

Section: Antibacterial and Antifungal Assaysmentioning

confidence: 99%

Micrococcin P1 and P2 from Epibiotic Bacteria Associated with Isolates of Moorea producens from Kenya

2022

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Epibiotic bacteria associated with the filamentous marine cyanobacterium Moorea producens were explored as a novel source of antibiotics and to establish whether they can produce cyclodepsipeptides on their own. Here, we report the isolation of micrococcin P1 (1) (C48H49N13O9S6; obs. m/z 1144.21930/572.60381) and micrococcin P2 (2) (C48H47N13O9S6; obs. m/z 1142.20446/571.60370) from a strain of Bacillus marisflavi isolated from M. producens’ filaments. Interestingly, most bacteria isolated from M. producens’ filaments were found to be human pathogens. Stalked diatoms on the filaments suggested a possible terrestrial origin of some epibionts. CuSO4.5H2O assisted differential genomic DNA isolation and phylogenetic analysis showed that a Kenyan strain of M. producens differed from L. majuscula strain CCAP 1446/4 and L. majuscula clones. Organic extracts of the epibiotic bacteria Pseudoalteromonas carrageenovora and Ochrobactrum anthropi did not produce cyclodepsipeptides. Further characterization of 24 Firmicutes strains from M. producens identified extracts of B. marisflavi as most active. Our results showed that the genetic basis for synthesizing micrococcin P1 (1), discovered in Bacillus cereus ATCC 14579, is species/strain-dependent and this reinforces the need for molecular identification of M. producens species worldwide and their epibionts. These findings indicate that M. producens-associated bacteria are an overlooked source of antimicrobial compounds.

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“…According to data in the literature, all characterised species have already been confirmed as plant growth-promoting streptomycetes (PGPS). In the case of Streptomyces albidoflavus [41][42][43], Streptomyces sampsonii [44,45], Streptomyces puniceus [46], Streptomyces cirratus [47], Streptomyces flavovariabilis [48] and Streptomyces rishiriensis [49], there was a strong antagonistic activity against several phytopathogenic fungi such as Aspergillus niger, Microsporum gypseum, Trichopyton sp., Fusarium sp. and Alternaria solani.…”

Section: Characterisation Of Actinomycete Strainsmentioning

confidence: 99%

The Impact of Growth-Promoting Streptomycetes Isolated from Rhizosphere and Bulk Soil on Oilseed Rape (Brassica napus L.) Growth Parameters

et al. 2021

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Inoculation of Streptomyces to improve oilseed rape (Brassica napus L.) yields and minimise the use of chemical fertilisers is a promising sustainable strategy. In this study, we isolated 72 actinobacterial strains from rhizosphere of oilseed rape and maize and from bulk soil for screening and characterising their antimicrobial activity. Nine promising strains, identified as Streptomyces sp. by morphology, physiological characteristics, and 16S rRNA gene sequencing, were selected for their plant growth-promoting traits and in planta experiments. The actinobacterial strains were positive for IAA production, siderophore production, and HCN production. In planta experiments were conducted by soaking the oilseed rape seeds in the actinobacterial suspension, followed by plant growth under controlled conditions in a cultivate chamber (22–28 °C, 8 h dark/16 h light, constant humidity 80%). We recorded root and shoot length (cm) and seedling fresh weight (g). For most of the abovementioned parameters, a significant enhancement was observed with strain KmiRC20A118 treatment. The length of the root increased by 53.14%, the shoot length increased by 65.6%, and the weight of the fresh plant by 60% compared to the control. The integrated application of PGPS (Plant Growth Promoting Streptomyces) from the rhizosphere of oilseed rape is a promising strategy to improve the growth of oilseed rape.

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Characterization of Antimycins – Producing Streptomycete Strain VY46 Isolated from Slovak Soil

Abstract: ABSTRACT

Cited by 5 publications

References 28 publications

Screening and Characterization of Streptomyces spp. Isolated from Three Moroccan Ecosystems Producing a Potential Inhibitor of the Drug Efflux Pump AcrAB-TolC

Screening and Characterization of Streptomyces spp. Isolated from Three Moroccan Ecosystems Producing a Potential Inhibitor of the Drug Efflux Pump AcrAB-TolC

Micrococcin P1 and P2 from Epibiotic Bacteria Associated with Isolates of Moorea producens from Kenya

The Impact of Growth-Promoting Streptomycetes Isolated from Rhizosphere and Bulk Soil on Oilseed Rape (Brassica napus L.) Growth Parameters

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