Biodiversity of Antimicrobial-Producing Actinomycetes Strains Isolated from Dry Dipterocarp Forest Soil in Northeast Thailand

Chanthasena, Panjamaphon; Nantapong, Nawarat

doi:10.1590/1678-4324-2016150674

Cited by 10 publications

(10 citation statements)

References 29 publications

(13 reference statements)

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“…Thus, 25, 20 and 12% of the strains had antimicrobial activity against B. subtilis, M. Loteus and S.aureus, respectively, while only 16% and 6% of the strains showed antimicrobial effect against E. coli and K. pneumonia, respectively. Similar results have been shown in other studies (Chanthasena and Nantapong 2016). Therefore, it may be concluded that gram-positive bacteria are more sensitive to antibiotic compounds than are gram-negative bacteria, and this is probably due to the presence of an outer membrane in latter's cell wall.…”

Section: Discussionsupporting

confidence: 90%

Actinomycetes of Howz Soltan Lake and their metabolite diversity in Iran

Salehghamari

Moradi

Sardabi

et al. 2022

Preprint

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Saline environments are largely unexplored sources for actinomycetes with the potential to produce biologically active secondary metabolites. A total of 34 actinomycete strains from water, sediments and mostly rhizosphere (82%) were collected from different sites of Howz Soltan Lake in Iran. Based on phylogenetic analysis, the isolated strains belonged to the genera Streptomyces, Nocardia and Saccharomonospora. Among the isolated actinomycetes, several strains had valuable biotechnological applications such as growth inhibition of human tumor cells. The results of cytotoxic assay revealed strain act9 having the most potent extract (19.716 ± 5.72 µg/ml) against the MDA-MB-231 human breast cancer cell. Also 38% of the strains had an antimicrobial activity against some pathogenic and nonpathogenic microorganisms. The ethyl-acetate extract of act18 showed the most antibacterial effect against Staphylococcus aureus, MRSA, and was further analyzed by GC/MS. Ar-tumerone (26.41%) and Butyl isodecyl phthalate (21.77%) were the main constituents detected in the extract.. This is the first time this compound is being detected in a prokaryote. Act18 showed the most 16s rRNA sequence similarity to Streptomyces youssoufiensis. In addition, a number of the isolates produced different enzymes including lipase, amylase, protease, gelatinase, urease and lecetinase. Also, the plant growth promoting activity of some actinomycete strains were shown. Some of the strains belonging to the genera Streptomyces and Nocardia increased the seed germination, stem length and the number of Echium leaves during the 20 days. Consequently, it was concluded that actinomycetes isolated from these saline lake habitats are potentially valuable for biotechnological applications.

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Section: Discussionsupporting

confidence: 90%

Actinomycetes of Howz Soltan Lake and their metabolite diversity in Iran

Salehghamari

Moradi

Sardabi

et al. 2022

Preprint

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“…The population of cellulase producing actinobacteria is reasonably high in forest floors and soil for the purpose of soil cycling and decomposition of tough plant materials and woody stems. These actinobacteria are largely responsible for the breakdown of large biopolymers like cellulose, hemicellulose, lignin and chitin 32,43,44 .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial biosynthetic potential and diversity of culturable soil actinobacteria from forest ecosystems of Northeast India

Sharma

Thakur²

2020

Sci Rep

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Actinobacteria is a goldmine for the discovery of abundant secondary metabolites with diverse biological activities. this study explores antimicrobial biosynthetic potential and diversity of actinobacteria from Pobitora Wildlife Sanctuary and Kaziranga National Park of Assam, India, lying in the Indo-Burma mega-biodiversity hotspot. A total of 107 actinobacteria were isolated, of which 77 exhibited significant antagonistic activity. 24 isolates tested positive for at least one of the polyketide synthase type I, polyketide synthase type II or non-ribosomal peptide synthase genes within their genome. their secondary metabolite pathway products were predicted to be involved in the production of ansamycin, benzoisochromanequinone, streptogramin using DoBISCUIT database. Molecular identification indicated that these actinobacteria predominantly belonged to genus Streptomyces, followed by Nocardia and Kribbella. 4 strains, viz. Streptomyces sp. PB-79 (GenBank accession no. KU901725; 1313 bp), Streptomyces sp. Kz-28 (GenBank accession no. KY000534; 1378 bp), Streptomyces sp. Kz-32 (GenBank accession no. KY000536; 1377 bp) and Streptomyces sp. Kz-67 (GenBank accession no. KY000540; 1383 bp) showed ~89.5% similarity to the nearest type strain in EzTaxon database and may be considered novel. Streptomyces sp. Kz-24 (GenBank accession no. KY000533; 1367 bp) showed only 96.2% sequence similarity to S. malaysiensis and exhibited minimum inhibitory concentration of 0.024 µg/mL against methicilin resistant Staphylococcus aureus ATCC 43300 and Candida albicans MTCC 227. This study establishes that actinobacteria isolated from the poorly explored Indo-Burma megabiodiversity hotspot may be an extremely rich reservoir for production of biologically active compounds for human welfare. Since ancient times, mankind has been exploring nature for bioactive organisms to treat different ailments. More than 100000 natural products have been identified in the last 150 years, which include highly assorted chemical classes such as polyketides, alkaloids, non-ribosomal peptides, isoprenoids or phenylpropanoids 1,2. Microbial biodiversity can provide us with the richest and the most versatile reservoir of potentially active natural products. A large portion of genomes from beneficial microbes are dedicated to the production of these valuable natural products. A single microbe is capable of making 30-50 natural product compounds 3. There are approximately 1 million natural products out of which about 25% are biologically active showing either positive activity or toxicity. Among the prospective sources of natural products, bacteria are prolific sources and a vast majority of these products are produced by a phylum of Gram-positive bacteria known as the "Actinobacteria" 4-6. Actinobacteria are diverse group of bacteria with high content of guanosine-cytosine (65-75%) ranging from 2.5-9.7 Mb genome size 7. Among actinobacteria, about 75% of antibiotics such as ivermectin, streptomycin, nystatin and tetracycline are produced by the microbial world's...

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“…According to a previous study, a total of 123 bacterial soil isolates were isolated from dry dipterocarp forest soil around Suranaree University of Technology, Nakhon Ratchasima, Thailand (14.8729° N, 102.0237° E) [ 26 ]. The isolates were tested for antimicrobial activity against test pathogens including Gram-positive and Gram-negative bacteria.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Bioactive Compounds from Streptomyces actinomycinicus PJ85 and Their In Vitro Antimicrobial Activities against Methicillin-Resistant Staphylococcus aureus

Chanthasena

Hua

Rosyidah³

et al. 2022

Antibiotics

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Antibiotic-resistant strains are a global health-threatening problem. Drug-resistant microbes have compromised the control of infectious diseases. Therefore, the search for a novel class of antibiotic drugs is necessary. Streptomycetes have been described as the richest source of bioactive compounds, including antibiotics. This study was aimed to characterize the antibacterial compounds of Streptomyces sp. PJ85 isolated from dry dipterocarp forest soil in Northeast Thailand. The 16S rRNA gene sequence and phylogenetic analysis showed that PJ85 possessed a high similarity to Streptomyces actinomycinicus RCU-197T of 98.90%. The PJ85 strain was shown to produce antibacterial compounds that were active against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA). The active compounds of PJ85 were extracted and purified using silica gel column chromatography. Two active antibacterial compounds, compound 1 and compound PJ85_F39, were purified and characterized with spectroscopy, including liquid chromatography and mass spectrometry (LC–MS). Compound 1 was identified as actinomycin D, and compound PJ85_F39 was identified as dihomo-γ-linolenic acid (DGLA). To the best of our knowledge, this is the first report of the purification and characterization of the antibacterial compounds of S. actinomycinicus.

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Biodiversity of Antimicrobial-Producing Actinomycetes Strains Isolated from Dry Dipterocarp Forest Soil in Northeast Thailand

Abstract: The increasing use of antibiotics has led to the development of drug-resistant microorganisms. The emergence of resistant strains has compromised the treatment and control of infectious diseases. As a result

Cited by 10 publications

References 29 publications

Actinomycetes of Howz Soltan Lake and their metabolite diversity in Iran

Actinomycetes of Howz Soltan Lake and their metabolite diversity in Iran

Antimicrobial biosynthetic potential and diversity of culturable soil actinobacteria from forest ecosystems of Northeast India

Isolation and Identification of Bioactive Compounds from Streptomyces actinomycinicus PJ85 and Their In Vitro Antimicrobial Activities against Methicillin-Resistant Staphylococcus aureus

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