Nanofibrous Scaffolds for Tissue Engineering Applications

Jaiswal, Amit

doi:10.1590/1678-4324-2016150644

Cited by 10 publications

(1 citation statement)

References 21 publications

(17 reference statements)

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“…In addition to its hemostatic role, it acts as an endogenous support matrix for the development of the initial phases of the tissue repair process. For these reasons, it is the natural polymer most used as a structural component in regenerative medicine and tissue engineering applied to the musculoskeletal system [13,14]. This autologous biomaterial, associated with platelets (PLA) and leukocytes (LEU), has the potential to act as a bioactive carrier, since these cellular components are able to release proinflammatory cytokines and/or growth factors responsible for the orchestrated modulation of process of tissue repair, stimulating events such as angiogenesis, cell proliferation and differentiation, and the formation of extracellular matrix [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Bioactive Fibrin Scaffolds for Use in Musculoskeletal Regenerative Medicine

Braga

Trovatti

Carvalho

et al. 2020

Braz. arch. biol. technol.

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Autologous fibrin matrices derived from the Leukocyte and Platelet Rich Plasma (L-PRP) and Leukocyte and Platelet Rich Fibrin (L-PRF) techniques present great potential to act as a bioactive scaffold in regenerative medicine, contributing to the maintenance of cell viability, proliferation stimulus and differentiation. In contrast, there are few studies that characterize the bioactive potential of these fibrin scaffolds by considering the process of production. The objective of this work was to characterize the intrinsic potential of maintaining cell viability of different fibrin scaffolds containing platelets and leukocytes. In order to achieve that, blood samples from a volunteer were collected and processed to obtain fibrin clots using the suggested techniques. To characterize the potential for in vitro viability, mesenchymal stem cells from human infrapatellar fat were used. The scaffolds were cellularized (1x10 5 cells/scaffolds) and maintained for 5 and 10 days under culture conditions with Dulbecco's Modified Eagle Medium, without addition of fetal bovine serum, and subsequently subjected to analyses by Fourrier transform infra-red spectroscopy, circular dichroism and fluorescence microscopy. The results demonstrated distinct intrinsic potential viability between the scaffolds, and L-PRP was responsible for promoting higher levels of viability in both periods of analysis. No viable cells were identified in the fibrin matrix used as controls. These results allow us to conclude that both fibrin substrates have presented intrinsic potential for maintaining cell viability, with superior potential exhibited by L-PRP scaffold, and represent promising alternatives for use as bioactive supports in musculoskeletal regenerative medicine.

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