Effect of Gamma Radiation on Growth and Metabolic Activities of Arthrospira platensis

Abomohra, Abd El‐Fatah; El-Shouny, Wagih A.; Sharaf, M. S.; Abo-Eleneen, Mai A.

doi:10.1590/1678-4324-2016150476

Cited by 26 publications

(12 citation statements)

References 29 publications

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“…varied, however, as Badri et al (2015) reported no significant impacts on chlorophyll when exposing Arthrospira cultures to similar doses used by El-Fatah Abomohra et al (2016). The authors reported a reduction in the antenna pigments allophycocynanin and phycocynanin in addition to an increasing lag phase in growth as the dose of radiation increased.…”

Section: Discussionmentioning

confidence: 94%

“…Previous studies investigating the effect of ionizing radiation on axenic cultures of cyanobacteria have reported similar drops in chlorophyll concentrations, but after much higher doses from a 60 Co-gamma radiation source. El-Fatah Abomohra et al (2016) reported up to a 25% reduction in the chlorophyll concentrations of Arthrospira platensis 15 days after exposure to 2.5 kGy of radiation. This coincided with a reduction in total biomass production by 34%.…”

Section: Discussionmentioning

confidence: 99%

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Radiation Tolerance of Pseudanabaena catenata, a Cyanobacterium Relevant to the First Generation Magnox Storage Pond

et al. 2020

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Recently a species of Pseudanabaena was identified as the dominant photosynthetic organism during a bloom event in a high pH (pH ∼11.4), radioactive spent nuclear fuel pond (SNFP) at the Sellafield Ltd., United Kingdom facility. The metabolic response of a laboratory culture containing the cyanobacterium Pseudanabaena catenata, a relative of the major photosynthetic microorganism found in the SNFP, to X-ray irradiation was studied to identify potential survival strategies used to support colonization of radioactive environments. Growth was monitored and the metabolic fingerprints of the cultures, during irradiation and throughout the post-irradiation recovery period, were determined using Fourier transform infrared (FT-IR) spectroscopy. A dose of 95 Gy delivered over 5 days did not significantly affect growth of P. catenata, as determined by turbidity measurements and cell counts. Multivariate statistical analysis of the FT-IR spectral data revealed metabolic variation during the post-irradiation recovery period, with increased polysaccharide and decreased amide spectral intensities. Increases in polysaccharides were confirmed by complementary analytical methods including total carbohydrate assays and calcofluor white staining. This observed increased production of polysaccharides is of significance, since this could have an impact on the fate of the radionuclide inventory in the pond via biosorption of cationic radionuclides, and may also impact on downstream processes through biofilm formation and biofouling.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Radiation Tolerance of Pseudanabaena catenata, a Cyanobacterium Relevant to the First Generation Magnox Storage Pond

et al. 2020

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“…Baicus & Baicus (2007) reported the antioxidant activity was attributed due to the presence of phenolic compounds in the methanol algal extract include of salicylic acid, chlorogenic, transcinnamic, caffeic acids, quimic and synaptic; individually or in a synergistic action. Abomohra et al . (2016) reported that gama radiation induce to the (ROS) cellular reactive oxygen species through the signaling purinergic which stimulate the Arthrospira platensis cells to increase the production of antioxidants under the oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Different Biological Activities of Spirulina platensis Extracts

El.Din

2019

Egypt. J. Bot.

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PIRULINA is aquatic and photosynthetic blue green algae and it is a good source of c-phycocyanin pigments. It is widely utilized as food supplement because it contains significant amount of proteins. This investigation is an effort to study some of medicinal applications of S. platensis such as antioxidant capacity, antimicrobial, cholesterol-reduction and anticancer. Antimicrobial activity of methanol, acetone, chloroform and petroleum ether extracts of dried S. platensis was assayed against two fungi (Aspergillus flavus and Aspergillus niger) and four bacterial species (Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhimurium). The S. platensis extracts exhibited significant activity against all tested bacteria and fungi. The highest inhibition zone (1.35 ±0.04cm) was observed in acetone extract against S. typhimurium. While, petroleum ether extract of S. platensis was recorded the minimum activities of all tested antimicrobial and the lowest inhibition zone against Staphylococcus aureus (0.52±0.01cm). The antioxidant potential of different solvent extracts clearly that acetone extract of S. platensis showed highest percentage of DPPH (2, 2 diphenyl-1picrylhydrazyl) scavenging activity (75%) and highest values of phenolic contents (0.562 mg/g DW). S. platensis displayed anticancer activity due to the productions of phycocyanin, allophycocyanin and other valuable products. Calf Serum was used to evaluate the potentiality of various S. platensis extracts to control the tumor effects in vitro. The cell proliferation assay showed that petroleum ether extract at a concentration of 125 µg ml-1 for 24hrs, significantly inhibited the growth of the Fetal Calf tumor cells (13% viability).

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“…Some of the bioactives associated with skincare are the algal proteins, lipids, vitamins and secondary metabolites such as phenolics, hydrocarbons, pigments etc [12]. (1.33 MeV) has an ability to induce cytological, anatomical, biochemical, morphological and physiological alterations in cells and tissues [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Effect of Gamma Irradiation on Antioxidant Potential and Bioactives of a Cosmeceutically Significant Chlorella Emersonii Kj725233

Sawant

Singh

Ghosh

et al. 2019

Int J Pharm Pharm Sci

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Objective : Gamma radiation induces free radicals with a corresponding alteration in the cell’s antioxidant defense system. The present study thus aimed at determining the role of gamma irradiation in improving the cosmeceutical potential of CEK in terms of antioxidants. Methods : C.emersonii KJ725233 was subjected to low (100 Gy) and high (1000 Gy) gamma irradiation doses and its effect on the chlorophyll content was evaluated. The quantitative alterations in the antioxidant content of CEK were evaluated by phosphomolybdenum assay (TAC), ferric reducing antioxidant potential (FRAP), 2, 2 - Dipheny-1-picryl hydrazyl radical scavenging assay (DPPH), total phenolic (TPC) and total flavonoid content (TFC). Also, the corresponding qualitative alterations in the bioactives of CEK were determined by GC-HRMS analysis. Results : A 179.57±2.55% increase in the total chlorophyll content alongwith a 71.76±2.96%, 32.08±2.16%, 11.67±0.89%, 42.85±8.0% and 31.37±3.18% increase was observed in the TAC, FRAP, DPPH radical scavenging, TPC and TFC was observed in CEK irradiated at 1000 Gy. GC-HRMS analysis revealed the induction of Vitamin E on irradiation at both the doses with a corresponding decrease in the phytol content whereas 100 Gy stimulated the induction of phytosterols. Conclusion : The potent intrinsic antioxidant activity of cosmeceutically significant CEK can be elevated with the induction of the most sought after antioxidant in cosmetology – Vitamin E on irradiation.

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Effect of Gamma Radiation on Growth and Metabolic Activities of Arthrospira platensis

Abstract: This work aimed to study the influence of gamma radiation on the growth and production of some active substances of Arthrospira platensis. Biomass production was significantly inhibited (p ≤ 0.05)

Cited by 26 publications

References 29 publications

Radiation Tolerance of Pseudanabaena catenata, a Cyanobacterium Relevant to the First Generation Magnox Storage Pond

Radiation Tolerance of Pseudanabaena catenata, a Cyanobacterium Relevant to the First Generation Magnox Storage Pond

Evaluation of Different Biological Activities of Spirulina platensis Extracts

Effect of Gamma Irradiation on Antioxidant Potential and Bioactives of a Cosmeceutically Significant Chlorella Emersonii Kj725233

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