HER-2 gene expression in atypical ductal hyperplasia associated with canine mammary carcinomas

Ferreira, Ênio; Bertagnolli, Angélica Cavalheiro; Gobbi, Helenice; Cassali, Geovanni Dantas

doi:10.1590/1678-41626212

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…identify a direct relationship between growth factor-mediated signaling and modulating extracellular matrix (ECM) components [ 2 ]. Also, some data have shown that human epidermal growth factor receptor, a family of tyrosine kinase receptors, expressed in normal tissues and in many types of cancer in humans [ 3 ] and canines [ 4 , 5 ] and its downstream pathway regulates migration and tumor invasion by ECM components [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland

et al. 2016

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Versican expression promotes tumor growth by destabilizing focal cell contacts, thus impeding cell adhesion and facilitating cell migration. It not only presents or recruits molecules to the cell surface, but also modulates gene expression levels and coordinates complex signal pathways. Previously, we suggested that the interaction between versican and human epidermal growth factor receptors may be directly associated with tumor aggressiveness. Thus, the expression of EGFR and HER-2 in these neoplasms may contribute to a better understanding of the progression mechanisms in malignant mammary tumors. The purpose of this study was to correlate the gene and protein expressions of EGFR and HER2 by RNA In Situ Hybridization (ISH) and immunohistochemistry (IHC), respectively, and their relationship with the versican expression in carcinomas in mixed tumors and carcinosarcomas of the canine mammary gland. The results revealed that EGFR mRNA expression showed a significant difference between in situ and invasive carcinomatous areas in low and high versican expression groups. Identical results were observed in HER-2 mRNA expression. In immunohistochemistry analysis, neoplasms with low versican expression showed greater EGFR immunostaining in the in situ areas than in invasive areas, even as the group presenting high versican expression displayed greater EGFR and HER-2 staining in in situ areas. Significant EGFR and HER-2 mRNA and protein expressions in in situ carcinomatous sites relative to invasive areas suggest that these molecules play a role during the early stages of tumor progression.

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Section: Introductionmentioning

confidence: 99%

HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland

et al. 2016

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“…The hybridized probe was detected using the CISH Polymer Detection Kit II, and the tissues were counterstained with Mayer's hematoxylin for 10 s. Previously tested human breast samples were used as positive controls. The performance of the CISH was performed according to the manufacturer's criteria and previous standardizations of our group [63].…”

Section: Chromogenic In Situ Hybridization (Cish)mentioning

confidence: 99%

Diverse roles of epidermal growth factors receptors in oral and cutaneous canine melanomas

et al. 2020

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Background: The epidermal growth factor receptors participate in the physiological processes such as regulation of morphogenesis, proliferation and cell migration, but when overexpressed or overactivated they may play an important role in neoplastic progression. Melanoma is the most aggressive skin neoplasm and is characterized by elevated invasion and low survival rates in both humans and dogs. In human melanomas the overexpression of EGFR, HER3 or HER4 is associated with poor prognosis. In canine melanomas the epidermal growth factor receptors expression has not been evaluated. Therefore, this study evaluated the expression of epidermal growth factor receptors by immunohistochemistry and investigated their relationship with morphological characteristics and proliferative indices in cutaneous and oral canine melanoma. Results: In cutaneous melanoma an increased proliferative index was associated with increased cytoplasmic HER4 and reduced EGFR and HER3 protein expression. In oral melanomas, membranous HER2 protein expression correlated with occurrence of emboli, but ERBB2 gene amplification wasn't observed. Conclusion: Thus, our work evidenced the relationship between HER4 and the stimulus to cell proliferation in cutaneous melanomas, in addition to the relationship between HER2 and the occurrence of emboli in oral melanomas.

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“…Different factors influence CMC development, including breed and genetic predisposition, age, reproductive history, hormonal activity, diet, and obesity (Sleeckx et al, 2011). Previous lesions, such as mammary atypical hyperplasia, can also increase the risk of CMC presentation (Dupont et al, 1993;Page et al, 2003;Ferreira et al, 2014).…”

Section: Cmc Risk Factors and Pathogenesismentioning

confidence: 99%

Canine mammary cancer

et al. 2020

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Canine mammary cancer (CMC) is one of the most common neoplasms in intact females in comparison to other species. Several risk factors have been identified, including breed, genetic predisposition, age, reproductive history, hormonal influence, diet, and body condition, in addition to previous lesions to the mammary gland, such as mammary atypical hyperplasia. An understanding of the genetic markers for the disease and a clinical approach are important for establishing a specific therapy that can allow adequate patient survivorship. Overexpression of the HER-2 gene in canines and humans is associated with a poor clinical prognosis, mainly short survivorship, although the clinical relationship is not clear. The incidence of HER-2 in female dogs can range from 29.7% to 38%. However, overexpression of HER-2 is not necessarily associated with malignancy processes of the mammary tissue, although it participates in cellular proliferation. Finally, canines remain one of the most important models for comparative oncology with humans due to the great similarity in the spontaneous presentation and development of cancer, and in the high homology in the amino acid sequence.

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HER-2 gene expression in atypical ductal hyperplasia associated with canine mammary carcinomas

Cited by 5 publications

References 10 publications

HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland

HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland

Diverse roles of epidermal growth factors receptors in oral and cutaneous canine melanomas

Canine mammary cancer

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