In vivo biocompatibility of nanostructured Chitosan/Peo membranes

Abstract

In vivo biocompatibility of nanostructured Chitosan/Peo membranes ABSTRACTElectrospinning is a technique that allows the preparation of nanofibers from various materials. Chitosan is a natural and abundant easily obtained polymer, which, in addition to those features, proved to be biocompatible. This work used nanostructured chitosan and polyoxyethylene membranes as subcutaneous implants in Wistar rats to evaluate the biocompatibility of the material. Samples of the material and tissues adjacent to the implant were collected 7, 15, 30, 45 and 60 days post-implantation. Macroscopic integration of the material to the tissues was observed in the samples and slides for histopathological examination that were prepared. It was noticed that the material does not stimulate the formation of adherences to the surrounding tissues and that there is initial predominance of neutrophilia and lymphocytosis, with a declining trend according to the increase of time, featuring a non-persistent acute inflammatory process. However, the material showed fast degradation, impairing the macroscopic observation after fifteen days of implantation. It was concluded that the material is biocompatible and that new studies should be conducted, modifying the time of degradation by changes in obtaining methods and verifying the biocompatibility in specific tissues for biomedical applications.

Cicatrização da musculatura reto-abdominal em coelhos submetidos à laparorrafia com fios de sutura à base de quitosana, categute cromado e poliglactina 910

“…Os fios de quitosana apresentaram índice de viabilidade de 30%, indicando que eles são tóxicos, visto que as células não conseguiram sobreviver quando em contato com o fio. De acordo com Vulcani et al (2015), a quitosana possui comprovada biocompatibilidade e atoxicidade, levando a relacionar a citotoxicidade encontrada no presente estudo ao processo de fabricação dos fios.…”

Polysaccharide-Based Membrane Biocompatibility Study of Anacardium occidentale L. and Polyvinyl Alcohol after Subcutaneous Implant in Rats

“…The other eighteen animals were part of the latex group (GL, positive control) and subjected to a surgical procedure followed by the implantation of a 9 mm diameter natural latex membrane used as a control for promoting an adequately reactive response in the biomaterial compatibility test system [27]. Each group was randomly divided into three subgroups, with six animals each, according to the times of euthanasia and sample collection at 15, 30, and 60 days after implantation [28].…”