Subclinical ketosis risk prediction in dairy cows based on prepartum metabolic indices

Wang, D.; Yu, Dan‐Na; Xia, Cheng; Xu, Chuang; Wu, Lina

doi:10.1590/1678-4162-12079

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…The results of this study showed that energy-related indices such as NEFA, BHB, glucose, insulin, and RQUICKI may not predict postpartum ketosis in primiparous cows. Because of the differences in parity, these results do not apply to previous potential warning techniques based on NEFA, BHB, and glucose ( Wang et al, 2021 ). Therefore, potential warning biomarkers of ketosis were identified in primiparous cows using metabolomic and microbiological approaches.…”

Section: Discussionmentioning

confidence: 70%

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Screening for potential warning biomarkers in cows with ketosis based on host–microbiota co-metabolism analysis

Du,

Luo,

Huang

et al. 2024

Front. Microbiol.

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IntroductionThe risk of ketosis is assessed by monitoring changes in plasma metabolites and cow behavior during the peripartum period. However, little is known about changes in the fecal bile acid and microbiota of cows before parturition. Therefore, this study clarified the bile acid profile and screened potential warning biomarkers in heifers 7 days before calving.MethodsNinety healthy cows were tracked in the transition period, and plasma and feces were collected 7 days before calving, on calving day, and 7 days after calving. The cows were divided into ketosis and healthy groups based on the blood β-hydroxybutyric acid levels from day 7 after calving. The levels of serum biochemical indices were measured at three time points using commercial kits. Ten cows in the ketosis group (KET-7) and 10 healthy cows (HEA-7) were randomly selected 7 days before calving for metabolome and 16S rRNA amplicon sequencing.ResultsNo significant differences in serum energy-related indices were observed 7 days before calving. The major bile acids in the feces of the KET-7 group were non-conjugated secondary bile acids (UnconSBA). Differential bile acids were primarily derived from UnconSBA. The potential ketosis warning metabolite in feces for 7 days before delivery was isodeoxycholic acid. The abundance of Rikenellaaceae-RC9-gut-group in the KET-7 group increased, whereas the abundance of Oscillospiraceae UCG-010 bacteria significantly decreased. Lactobacillus and Prevotella-9 in feces were potential warning biomarkers for ketosis in dairy cows 7 days before calving. The variation in differential bile acids in the plasma, consistent with the feces, was mainly derived from UnconSBA. Lithocholic acid in the plasma was a potential ketosis warning metabolite 7 days before delivery.ConclusionKetotic cows experienced bile acid metabolism disorders 7 days before calving, and the gut microbiota was closely related to bile acid metabolism disorders. Future studies should investigate the relationship between secondary bile acids and the development of ketosis.

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Section: Discussionmentioning

confidence: 70%

“…Because of the differences in parity, these results do not apply to previous potential warning techniques based on NEFA, BHB, and glucose (Wang et al, 2021). Therefore, potential warning biomarkers of ketosis were identified in primiparous cows using metabolomic and microbiological approaches.…”

Section: Discussionmentioning

confidence: 91%

Screening for potential warning biomarkers in cows with ketosis based on host–microbiota co-metabolism analysis

Du,

Luo,

Huang

et al. 2024

Front. Microbiol.

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“…BHB levels in the blood of heifers ranged from 0.43 to 0.59 mmol/L 5-7 days before calving. According to Wang et al (2021), exceeding the level of 0.43 mmol/L for BHB and 0.27 mmol/L for NEFA before calving indicates a high risk of developing subclinical ketosis in the postpartum period. In our study, 20% of heifers had BHB levels above 1.0 mmol/L within 2-3 days after calving, and this number increased to 40% within 7-10 days.…”

Section: Discussionmentioning

confidence: 99%

“…Some researchers indicate that during the prepartum period, there are various changes in blood parameters, particularly, AST activity and NEFA levels, which can be used as risk indicators for predicting the occurrence of subclinical ketosis in cows after calving (Wang et al, 2021). Biochemical markers one week after calving had high prognostic value for the development of displaced abomasum (with BHB concentration above 1000 µmol/L, the odds were 13.6 times higher) and for the development of clinical ketosis (with serum NEFA above 1.0 mmol/L the odds were 6.3 times higher, and 4.7 times higher with BHB concentration above 1200 µmol/L) (Seifi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Biochemical abnormality in the Brown Swiss breed cows affected with ketosis in the perinatal period

Yefimov

2023

TAVM

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Ketosis is widespread disturbance among dairy cows, causing significant economic losses and requiring further study of its pathogenesis. This particularly applies to less traditional dairy breeds, including the Brown Swiss. The aim of the study was to investigate the biochemical indicators in the Brown Swiss breed cows of different age at a stage with prevalent ketosis. The research was conducted on a dairy cow herd with a ketosis level exceeding 30%. Blood samples were collected from primiparous cows and cows in their 2nd-3rd lactation 5-7 days before calving (group BC), post calving 2-3 days (group 2-3 PC), and 7-10 days (group 7-10 PC). Biochemical indicators of blood serum aimed at determining the level of lipomobilization, ketone bodies, and liver function were determined using an automatic biochemical analyzer. In the group of primiparous cows 2-3 PC, there was an increase in the content of beta-hydroxybutyrate by 1.83 times, and ketonemia was observed in 40% of cows. The postpartum period in primiparous cows was accompanied by an increase non-esterified fatty acids concentration by 1.75-2 times. The level of AST was the lowest in the serum of gestating cows several days before calving, whereas after calving, an increase was observed. It was discovered that each group had animals with AST levels above 100 U/L. There were 20 % of animals in the 5-7 pre-calving group, and 80% in the 7-10 post-calving group. The level of total bilirubin in the group of primiparous cows 2-3 PC exceeded the level BC by 3.14 times, and in the group 7-10 post-calving by 2.67 times. In the BC group, 20% of cows during their 2nd-3rd lactation were found to have ketonemia, with beta-hydroxybutyrate level 1.43 mmol/L in the serum. The highest level of ketone bodies was detected in cows from the group 2-3 PC, with 60% showing subclinical and 20% showing clinical ketosis. Similarly to primiparous cows, cows in their 2nd-3rd lactation exhibited the development of negative energy balance in the postpartum period. In the group of cows BC, AST activity exceeded 100 U/L in 20% of animals, while in the groups 2-3 PC and 7-10 PC, the number of such animals reached 60%. These changes were accompanied by hyperbilirubinemia, which was observed after calving in 80% of cows in both groups. Therefore, an increase non-esterified fatty acids levels was observed in the postpartum period in primiparous cows and cows in their 2nd-3rd lactation of the Brown Swiss breed. The results indicate the development of lipomobilization syndrome. The most pronounced signs of ketosis development were observed in primiparous cows on days 7-10 after calving, while in multiparous cows, it was detected on days 2-3. The development of negative energy balance led to signs of liver damage characteristic of lipidosis -an increase in total bilirubin levels and, to a lesser extent, AST activity. Biochemical index changes during post-calving period were observed in primiparous cow with more prominent level. However, during late dry period metabolic disorders were detected in mu...

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“…Increased lipomobilization and ketogenesis lead to fatty liver syndrome (decrease in cholesterol and triglyceride levels in the blood), along with hepatocyte necrosis syndrome [increased activity of the liver enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT) and gamma-glutamyl transferase (GGT)], biliary retention syndrome (increased total bilirubin) decreased hepatocyte secretion syndrome (decreased albumin value), and insulin resistance (Delić et al, 2020). Recent results have shown that these and many other parameters deviate in their values during ketosis in cows (Wang, 2021;Dervishi et al, 2021) The aim of this study is to examine the interrelationships and significance of blood parameters in the assessment of BHB values in healthy and ketotic cows using multiparameter statistics.…”

Section: Introductionmentioning

confidence: 99%

Multiparametric analysis of blood parameters and hyperketonemia in cows

Cincović¹,

Delić-Vujanović²,

Đoković³

et al. 2021

Acta agriculturae Serbica

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The aim of this study is to examine the interrelationships and importance of biochemical and endocrine blood parameters in the assessment of beta-hydroxybutyrate (BHB) values in healthy and ketotic cows using multiparameter statistics. The experiment included 45 Holstein Friesian cows (22 healthy and 23 with ketosis). The criterion used for detecting ketosis was the value of BHB ˃1.2 mmol/L. Based on laboratory indicators, cows were precisely classified into two large clusters: a cluster of healthy cows and a cluster of cows suffering from ketosis with minimal mixing of individual cows between clusters. Metabolic parameters were divided into two large clusters: parameters whose values increased in ketosis and decreased in healthy animals and parameters whose values decreased in ketosis and increased in healthy individuals. In ketotic cows there was a higher expression of non-esterified fatty acids, total bilirubin, aspartate aminotransferase, insulin and growth hormone, and a lower expression of glucose, albumin, triglycerides, cholesterol and total lipids compared to the healthy group. In 3 cows with ketosis, greater metabolic similarity with healthy cows was found, because of the absence of pronounced changes in the concentration of hormones and glucose. Thyroxine and triiodothyronine showed either increased or decreased expression in ketotic cows. In cows with lower values of these hormones, there were more pronounced metabolic changes characteristic of ketosis. For the development of metabolic adaptations to ketosis, in addition to hyperketonemia, there must be endocrine changes and changes in glycemia.

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Subclinical ketosis risk prediction in dairy cows based on prepartum metabolic indices

Cited by 5 publications

References 17 publications

Screening for potential warning biomarkers in cows with ketosis based on host–microbiota co-metabolism analysis

Screening for potential warning biomarkers in cows with ketosis based on host–microbiota co-metabolism analysis

Biochemical abnormality in the Brown Swiss breed cows affected with ketosis in the perinatal period

Multiparametric analysis of blood parameters and hyperketonemia in cows

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