Evaluation of stallion sperm motility with ImageJ using a cell phone camera and a light microscope

Freitag, G.; Lima, Luis Gustavo Freitag de; Kozicki, Luiz Ernandes; Felicio, Liedge Camila Simioni; Weiss, Romildo Romualdo

doi:10.1590/1678-4162-11934

Cited by 2 publications

(1 citation statement)

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“…Moreover, the manual or visual counting of seeds is tiring, repetitive, inefficient and increases the chances of human error, which can be overcome with the use of image processing techniques (Mussadiq et al, 2015;Acosta et al, 2017;Deng et al, 2021). ImageJ® is a free access software with several applications in image analysis in agrarian and biological sciences (Noronha et al, 2019;Freitag et al, 2020;Medeiros et al, 2020;Silva et al, 2020;Oliveira et al, 2021;Ribeiro et al, 2021). Among them, the possibility of counting elements in an image for different purposes (Passoni et al, 2014;Gautier and Ginsberg, 2021;Tsuzuki et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Thousand-seed weight determination in forest species by image analysis

et al. 2021

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Thousand-seed weight (TSW) is used to calculate the sowing density, number of seeds present in a sample, and predict seed size. Our aim was to establish an alternative methodology for thousand-seed weight determination in forest species using image analysis techniques. The traditional methodology was compared with the alternative one, testing them on seeds from 16 forest species. The traditional method was performed by manually counting eight repetitions of 100 seeds, weighing of samples, and computation of thousand-seed weight. The alternative methodology was performed by counting seed samples captured by images and processing in ImageJ® software, followed by weighing a single sample, and computation. All steps were timed, and each methodology was repeated ten times per species. The TSW obtained by the alternative methodology was similar to that obtained by the traditional one, and the average execution time of the activities was reduced by 62%. The proposed thousand-seed weight determination by image analysis is an efficient and optimized alternative to the traditional method. Thousand-seed weight determination from image analysis reduces execution time for the seed analyst and forest seedling producer.

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Section: Introductionmentioning

confidence: 99%

Thousand-seed weight determination in forest species by image analysis

et al. 2021

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The effects of solutions of maca (Lepidium meyenii) powder as a food/feed supplement on the viability of murine macrophage cells by digital image analysis

Korkmaz

Parmaksiz

Sait

et al. 2021

Journal of Advances in VetBio Science and Techniques

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The effects of solutions of maca (Lepidium meyenii) powder as a food/feed supplement on the viability of murine macrophage cells by digital image analysis ABSTRACT Maca (Lepidium meyenii) is a tuber root plant and belong to Brassicaceae family and recently used as a supplement in human and animal nutrition. In this study, it was aimed to investigate the cytotoxicity of two solutions (aqueous and ethanol) of maca root powder by digital image analysis. Maca powder was mixed in ultra distilled water and ethanol (1:2 w/v) for 24 h at 4 °C. The mixtures were centrifuged and the supernatants were ten-fold diluted for cytotoxic analysis of Raw 264.7 murine macrophage cells. After seeded the cell cultures in microplates, ten-fold dilutions (from 10 -1 to 10 -7 ) of both maca solutions were added as six replicates for 24 h. While the aqueous maca solution increased the number of dead cells at 10 -1 (50 mg mL -1 ), the ethanolic solution statistically increased the number of dead cells at 10 -1 , 10 -2 and 10 -3 (50, 5 and 0.5 mg mL -1 ) (P<0.01). In conclusion, the alcoholic preparation of maca powder caused a higher cytotoxic effect on the murine macrophage cells than the aqueous preparation due to the solvents and the dilution factor in this study.

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Evaluation of stallion sperm motility with ImageJ using a cell phone camera and a light microscope

Cited by 2 publications

References 16 publications

Thousand-seed weight determination in forest species by image analysis

Thousand-seed weight determination in forest species by image analysis

The effects of solutions of maca (Lepidium meyenii) powder as a food/feed supplement on the viability of murine macrophage cells by digital image analysis

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