Seroprevalence of Toxoplasma gondii and associated hematological alterations in small ruminants of D.G. Khan district of Southern Punjab, Pakistan

Abstract

The present study was carried out to evaluate the prevalence and hematological effects of Toxoplasma gondii in sheep and goat in district Dera Ghazi Khan. Blood samples (n=204) were collected comprise goats (n=101) and sheep (n=103) alongwith age, gender and breeds of animals. Samples were collected randomly from 25 flocks of 7 different union council Viz. Vehova, Tibbi Qaisrani, Lakhani, Kohar, Tuman Qaisrani, Nutkani and Kot Qaisrani of Tehsil Taunsa Sharif at least 4 animals from each flock. All ruminants divide into three groups based on age, breed and gender. The prevalence was detected through two different kits Viz. LAT and ELISA kit. The overall prevalence suspected in goats through LAT and ELISA kit was (35.64%), (32.67%) and in sheep was (25.24%), (23.30%) respectively. The Toxoplasma gondii had a significant effect on goats in age groups and non-significant all other groups of goats and sheep. Toxoplasma gondii had a significant effect on all hematological parameters like Hemoglobin, total leukocyte cells, granulocytes, lymphocytes, platelets, and red blood cells, except monocytes. In conclusion of the current study, toxoplasmosis is prevalent among ruminants, reveals the possibility of transmission to humans on the use of host animals as protein source.

Serological Investigation of Bovine Toxoplasmosis in Exotic Breed by Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA Kit

“…toxoplasmosis. In our ndings,35.75% of cattle were positive for T. gondii IgG by using LAT. It has already been used by other researchers to detect T. gondii antibodies (16.9% reported by Akinwumi et al; 10.1% reported by Okewole et al) who found a comparatively less seropositivity of cattle to toxoplasmosis (Joshua and Akinwumi 2003; Okewole 2007) as compared to ours.…”

“…The plate was Ochi et al 2013). The seroprevalence was compared by using Chi-square through SPSS(Lashari et al 2020). Total 400 samples were evaluated through performing LAT and our new ELISA kit.…”

Serological Investigation of Bovine Toxoplasmosis Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA Kit

“…ROC curve was used to determine the cut-off value of the Cattle Toxo IgG ELISA kit [ 53 ]. The seroprevalence was compared by using Chi-square through SPSS [ 54 ].…”