Hyperbaric oxygen therapy in wound healing in mice

Abstract

We investigated the local and systemic effects of hyperbaric oxygen therapy in BALB/C mice, exposed to two different exposure times, under 2.4 atmosphere (ATM). Fifteen animals were divided into three groups (GI, GII and Control) and underwent a surgical excision of a skin fragment of approximately one square centimeter of the dorsal region. The wounds were treated and monitored for 21 days. In the control group, the wound was cleaned once a day with sterile 0.9% NaCl solution. GI and GII mice were submitted to daily hyperbaric oxygen therapy of 30 or 60minutes sessions, respectively. The wounds were photographed every three days and their surfaces were analyzed by an image analyzer. At 21 days, all animals were euthanatized for histopathological analysis of the skin, lungs and liver in order to identify eventual alterations in wound healing or in the analyzed organs. Animals belonging to GI showed a faster skin wound healing in comparison to the other groups. Animals from GII, however, showed a delayed wound healing process and exhibited lung and microcirculatory alterations. These findings allow us to conclude that the exposure time to the oxygen in hyperbaric environment is crucial and can help or disturb skin wound healing or even be deleterious to other organs.

Hyperbaric Oxygen Therapy in Systemic Inflammatory Response Syndrome

“…In this study, the duration of HBOT treatments was 60 to 90 min, in compliance with Amaral et al (2021) [66] and McCurdy et al (2021) [22], who stated that the duration of the treatments is important for clinical success.…”

Avanços no tratamento de feridas: Parte 2

“…Contudo, é fundamental enfatizar que o uso indiscriminado da terapia hiperbárica pode acarretar em riscos, como barotraumas que resultam em lesões nos tímpanos e pulmões, além de toxicidade após exposição prolongada a altas concentrações de oxigênio. É relevante salientar que, na medicina veterinária, ainda não há consenso sobre protocolos terapêuticos estabelecidos, destacando a necessidade de novos estudos para sua padronização (AMARAL et al, 2021).…”