Inhibition of the growth of major mastitis-causing pathogens by non-aureus Staphylococcus isolates using the cross-streaking method

Ferronatto, José Augusto; Souza, Fernando Nogueira de; Libera, Alice Maria Melville Paiva Della; Vliegher, Sarne De; Visscher, Alex De; Piepers, Sofie; Blagitz, Maiara Garcia; Heinemann, Marcos Bryan

doi:10.1590/1678-4162-11006

Cited by 7 publications

(9 citation statements)

References 10 publications

(17 reference statements)

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“…The authors observed pronounced inhibitory effects of S. chromogenes isolates against S. aureus, variable effects against S. dysgalactiae and S. uberis, and no effects against E. coli, suggesting that this susceptibility to NAS is more pronounced against phylogenetically-related bacterial species (i.e., Gram positive). Other studies also reported only a modest effect against Gram-negative bacteria; however, this effect was observed by using the semiquantitative growth inhibition assay [15,23,24,31]. On the other hand, bacterial growth can also be limited by the availability of nutrients, which differs according to the local environment.…”

Section: Discussionmentioning

confidence: 99%

“…It is a common finding that NAS isolated from TA are more likely to produce antagonist substances against other bacteria [15,29]. Moreover, protection against S. aureus by the S. simulans species has previously been suggested [18,30], although most of the studies have reported more pronounced inhibitory effects by S. chromogenes [15,23,24,29] and a modest effect by S. epidermidis isolates. The effect observed for the latter species is most probably because these species are usually transferred from human skin to cows during the milking process [37,38] and their virulence properties are limited [39].…”

Section: Discussionmentioning

confidence: 99%

“…Antimicrobials are used in the context of mastitis on dairy herds [21], thus novel strategies to tackle an IMI away from antimicrobials are needed, especially for an IMI caused by methicillin-resistant Staphylococcus aureus (MRSA) [22]. It has been shown that certain bovine NAS can inhibit the in vitro growth of major mastitis bacterial pathogens, including S. aureus, Streptococcus (S.) dysgalactiae, S. uberis [15,23], and even Escherichia (E.) coli [24], opening venues for probiotic approaches for bovine mastitis prevention and control [25]. The method previously used [15,23,24] is a semiquantitative method that can be employed to screen a large group of isolates, relatively fast for their ability to inhibit the growth of major mastitis bacterial pathogens, especially those that are Gram positive, yet we hypothesize that a novel quantitative method would allow us to interpret (more) intra-and inter-species variations between NAS and also their effect against other bacterial strains.…”

Section: Introductionmentioning

confidence: 99%

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Novel Quantitative Assay to Describe In Vitro Bovine Mastitis Bacterial Pathogen Inhibition by Non-aureus Staphylococci

Silva

Beuckelaere

Visscher³

et al. 2022

Pathogens

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In this paper, we describe a new quantitative method to evaluate and quantify in vitro growth inhibition of mastitis-related bacteria. Colony-forming units of Staphylococcus (S.) aureus (n = 10), Escherichia (E.) coli (n = 10), and Streptococcus (S.) uberis (n = 10) were quantified after their growth on top of layers of trypticase soy agar (TSA) containing six different concentrations (varying from 102 to 107 CFU/mL) of bovine non-aureus staphylococci (NAS), i.e., S. chromogenes (n = 3) and S. simulans (n = 3) isolates. Growth inhibition of the mastitis-related major bacterial pathogens, including E. coli, was confirmed by all NAS, an effect that varied highly among NAS isolates and was not evident from the semiquantitative method with which the new method was compared. By subsequent application of the new method on a larger set of 14 bovine NAS isolates, we observed that S. simulans and NAS originating from teat apices (especially S. epidermidis) required lower concentrations to inhibit both methicillin-sensitive (MSSA) (n = 5) and methicillin-resistant S. aureus (MRSA) isolates (n = 5) originating from milk. Therefore, the new assay is a promising tool to precisely quantify the intra- and inter-species differences in growth inhibition between NAS.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel Quantitative Assay to Describe In Vitro Bovine Mastitis Bacterial Pathogen Inhibition by Non-aureus Staphylococci

Silva

Beuckelaere

Visscher³

et al. 2022

Pathogens

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“…The presence of non-aureus staphylococci (NAS) such as S. chromogenes on the teat apex during prepartum has been associated with lower somatic cell counts shortly after calving, which is used as an indicator for subclinical mastitis (De Vliegher, 2003). S. chromogenes isolated from the teat apex has also been shown to partially or fully inhibit S. aureus in vitro (Ferronatto et al, 2019). The reason for this apparent inhibition is thought to be the production of antimicrobial peptides (AMPs) capable of targeting and attenuating growth of other microorganisms (Cotter et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Exploring associations between the teat apex metagenome andStaphylococcus aureusintramammary infection risk in primiparous cows under organic directives

Dean,

Peña-Mosca,

Ray

et al. 2023

Preprint

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The primary objective of this study was to identify associations between teat apex microbiome and Staphylococcus aureus intramammary infection (IMI) risk in primiparous cows during the first 5 weeks after calving. We performed a case-control study using shotgun metagenomics of the teat apex and culture-based milk data collected longitudinally from 710 primiparous cows on 5 organic dairy farms. We observed a strong association between S. aureus DNA in the metagenomic teat apex data prior to parturition and the odds of S. aureus IMI after parturition (OR = 38.9, 95% CI: 14.84-102.21). Differential abundance analysis confirmed this association, with cases having a 23.8 higher log fold change (LFC) in abundance of S. aureus in their samples compared to controls. Of the most prevalent microorganisms in controls, those associated with a lower risk of post-calving S. aureus IMI included Microbacterium phage Min 1 (OR = 0.37, 95% CI: 0.25-0.53), Corynebacterium efficiens (OR = 0.53, 95% CI: 0.30-0.94), Kocuria polaris (OR = 0.54, 95% CI: 0.35-0.82), Micrococcus terreus (OR = 0.64, 95% CI: 0.44-0.93) and Dietzia alimentaria (OR = 0.45, 95% CI: 0.26-0.75). Microcin B17 was the most prevalent antibacterial peptide on the teat apex of cases and controls (99.7% in both groups). The predicted abundance of Microcin B17 was also higher in cases compared to controls (LFC 0.26). Cow and farm random effects often explained a large proportion of the observed variability in the teat apex microbiome, suggesting that our results need to be interpreted within the context of the random effects.

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“…They are commonly considered to be minor mastitis pathogens causing only a slight increase of the milk somatic cell count (SCC) [ 10 – 12 ] with no impact on milk yield. Other studies have suggested a protective effect of bovine NAS against, among other bacteria, S. aureus [ 13 – 17 ], including the repression of agr related virulence factors of S. aureus [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Bovine-associated non-aureus staphylococci suppress Staphylococcus aureus biofilm dispersal in vitro yet not through agr regulation

Silva

Souza

Mertens

et al. 2021

Vet Res

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Biofilm formation is a significant virulence factor in Staphylococcus (S.) aureus strains causing subclinical mastitis in dairy cows. A role of environmental signals and communication systems in biofilm development, such as the agr system in S. aureus, is suggested. In the context of multispecies biofilm communities, the presence of non-aureus staphylococci (NAS) might influence S. aureus colonization of the bovine mammary gland, yet, such interspecies interactions have been poorly studied. We determined whether 34 S. chromogenes, 11 S. epidermidis, and 14 S. simulans isolates originating from bovine milk samples and teat apices (TA) were able to affect biofilm formation and dispersion of S. aureus, and if so, how isolate traits such as the capacity to regulate the S. aureus agr quorum sensing system are determinants in this process. The capacity of an agr-positive S. aureus strain to form biofilm was increased more in the presence of S. chromogenes than in the presence of S. simulans and S. epidermidis isolates and in the presence of NAS isolates that do not harbor biofilm related genes. On the other hand, biofilm dispersion of this particular S. aureus strain was suppressed by NAS as a group, an effect that was more pronounced by isolates from TA. Furthermore, the observed effects on biofilm formation and dispersion of the agr-positive S. aureus strain as well as of an agr-negative S. aureus strain did not depend on the capacity of NAS to repress the agr system.

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Inhibition of the growth of major mastitis-causing pathogens by non-aureus Staphylococcus isolates using the cross-streaking method

Cited by 7 publications

References 10 publications

Novel Quantitative Assay to Describe In Vitro Bovine Mastitis Bacterial Pathogen Inhibition by Non-aureus Staphylococci

Novel Quantitative Assay to Describe In Vitro Bovine Mastitis Bacterial Pathogen Inhibition by Non-aureus Staphylococci

Exploring associations between the teat apex metagenome andStaphylococcus aureusintramammary infection risk in primiparous cows under organic directives

Bovine-associated non-aureus staphylococci suppress Staphylococcus aureus biofilm dispersal in vitro yet not through agr regulation

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