2018
DOI: 10.1590/1678-4162-10197
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Refrigeração do sêmen da garoupa-verdadeira Epinephelus marginatus

Abstract: RESUMO Foram estudados diferentes diluentes no processo de refrigeração do sêmen da garoupa-verdadeira. Inicialmente, a taxa e a duração da motilidade e a concentração espermática foram avaliadas, caracterizando a qualidade do sêmen fresco. Para os testes de refrigeração a 4ºC, diferentes diluentes foram testados em atmosfera normal e modificada (100% oxigênio). O sêmen fresco apresentou concentração espermática de 3,1±0,2 x 109 células mL-1, motilidade média de 90%, permanecendo móvel, em média, por 3.060 seg… Show more

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Cited by 2 publications
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“…Refrigeration at temperatures close to 4°C is one of the techniques commonly adopted to preserve semen for short periods, to keep semen quality feasible for hours, days and even weeks, maintaining its fertilization capacity (Contreras et al, 2019;Shaliutina et al, 2013). For this to occur, it is necessary to use diluting solutions that provide a protective medium, while both nourishing and reducing cell metabolism of sperm during storage conditions (Silva et al, 2018).Studies with fish have demonstrated the positive effects of refrigeration associated with the use of cryoprotectants among which…”
mentioning
confidence: 99%
“…Refrigeration at temperatures close to 4°C is one of the techniques commonly adopted to preserve semen for short periods, to keep semen quality feasible for hours, days and even weeks, maintaining its fertilization capacity (Contreras et al, 2019;Shaliutina et al, 2013). For this to occur, it is necessary to use diluting solutions that provide a protective medium, while both nourishing and reducing cell metabolism of sperm during storage conditions (Silva et al, 2018).Studies with fish have demonstrated the positive effects of refrigeration associated with the use of cryoprotectants among which…”
mentioning
confidence: 99%