Mastitis in the transition period: identification of potential blood markers

Santos, Karina Cecília Panelli; Souza, Fernando Nogueira de; Blagitz, Maiara Garcia; Batista, Camila Freitas; Bertagnon, Heloisa Godoi; Gomes, Renata Nascimento; Molinari, Paula; Diniz, S.A.; Silva, M.X.; Haddad, João Paulo Amaral; Heinemann, Marcos Bryan; Libera, Alice Maria Melville Paiva Della

doi:10.1590/1678-4162-10069

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…This is despite the fact that values recorded for mastitic does were slightly higher (4.11±0.31) than the non-mastitic does (3.46±0.28). This is in contrast with some previous works that reported a decrease in serum albumin concentration of mastitic ewes [38]. The increase noted in the serum ALP levels of mastitic and non-mastitic was not significant.…”

Section: Discussioncontrasting

confidence: 99%

Effect of mastitis on some hematological and biochemical parameters of Red Sokoto goats

et al. 2019

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Aim: This research project investigates the effect of mastitis on some hematological and biochemical parameters of Red Sokoto goats (RSGs). Materials and Methods: In this investigation, 16 clinically and subclinically diagnosed mastitic and 20 non-mastitic RSGs, within Sokoto metropolis, were sampled. Blood samples were collected both in ethylenediaminetetraacetic acid and anticoagulant free sample bottles for hematology and biochemical analysis, respectively. Results: A statistical analysis of the results revealed no significant difference in all the hematological parameters analyzed for both the mastitic and non-mastitic goats except mean corpuscular hemoglobin where significant difference (p<0.05) was observed. Similarly, no significant difference was recorded in the serum biochemistry except for the increase in total protein (p<0.001), globulin (p<0.05), and alanine aminotransferase activity (p<0.05). Conclusion: This finding is a pointer to the fact that mastitis could be regarded as a localized problem affecting the udder without serious systemic or metabolic involvement in RSGs.

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Section: Discussioncontrasting

confidence: 99%

Effect of mastitis on some hematological and biochemical parameters of Red Sokoto goats

et al. 2019

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“…For hematological and biochemical tests 10 blood samples were collected from jugular vein using sterile syringe pour 2 ml into the EDTA tube to estimate blood parameters including HB, PCV, TLC ,Monocyte and Lymphocytes, rather than the rest blood residue into get disposable tube for biochemical profile testing which used autamatomic biochemical cobas commercially obtained ROACH made by Germany used for biochemistry examination ALT, AST, ALP, Ca , Cl, Mg, zn [8].…”

Section: Blood Samples Collectionmentioning

confidence: 99%

Evaluation Effects of Coliform Mastitis on Hematological and Biochemical Parameters in Dairy Cows, in Kirkuk City, Iraq

Al-Bayati

2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study conducted to investigate sub clinical mastitis caused by some coliform pathogens as a common causes mastitis in the cows and to detect some hematological & biochemical parameters, for this purpose 100 milk samples and100 blood samples were collected from apparently cows brought to the veterinary hospital & from different heifer’s in Kirkuk governate, Iraq. our results showed that the incidence of coliform mastitis by using different diagnostic methods. the incidence mastitis to CMT according of management & hygiene milk of processing recorded 35 (42.6 %), 47 ( 57.3%) respectively., However the bacteriologically examination depending on isolation, biochemical manually and vitek system identification. revealed, the incidence as 82.9% positive for bacterial identification reveled that E.coli was predominant ( 33.8 %) bacteria Followed by K. pneumonia and. Proteus mirabilis (11.7 %) &(10.2)% respectively. further hematological parameters we noted that showed anemia with significant decrease (p<0.05) in Hemoglobin, Total red blood cell count, Paced cell volume, lymphocyte and slightly decrease in, while record increase in granulocyte and Wight blood cell count. On the other hand the biochemical profile we showed significant increase in ALT, AST enzymes, While decrease significant(p<0.05) in ALP, Ca, Cl, P, Mg & zn. We conclude from this study, The prevalence of pathogenic bacteria in the milk resulted in a significant alteration in the hamato-biochemical profile in the current study reveled significant monitors for Bovine mastitis and estimate the efficiency and quality of milk consumption.

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“…The transition period in dairy cows between the 3 weeks preceding calving and 3 weeks after calving is characterized by drastic metabolic and immune response changes. During this period, dairy cows are usually in a state of negative energy balance that results in substantial lipid mobilization, a drop in glucose levels, and a dramatic rise in nonesterified fatty acid (NEFA) and β-hydroxybutyrate (BHBA) levels, which impairs immune cell functions (1,2). Thus, dairy cows face the highest incidence of infectious diseases and metabolic disorders during this period (3,4).…”

Section: Introductionmentioning

confidence: 99%

Brief Research Report: Expression of PD-1 and CTLA-4 in T Lymphocytes and Their Relationship With the Periparturient Period and the Endometrial Cytology of Dairy Cows During the Postpartum Period

et al. 2022

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The present study sought to evaluate the expression of PD-1 and CTLA-4 in blood T lymphocytes during the periparturient period and their relationship with uterine health in dairy cows, as determined by endometrial cytology and serum concentrations of β-hydroxybutyrate (BHB) and non-esterified fatty acids (NEFAs), which are indicators of a negative energy balance. The second objective of this study was to investigate whether the expression of PD-1 and CTLA-4 in T lymphocytes is associated with the serum acute phase-protein haptoglobin concentration during the periparturient period. To address these objectives, 26 clinically healthy dairy cows were used. Peripheral blood was collected 14 days prepartum (T-14), at calving (T0), and 30 days postpartum (T30) to measure the expression of PD-1 and CTLA-4 in blood T lymphocytes by flow cytometry. In addition, we collected blood at T0, 10 days after parturition (T10), and T30 to obtain serum and determine the serum concentrations of NEFA, BHB, and Hp. Endometrial cytology was performed at T10, 20 days after parturition (T20), and T30. In the present study, we observed higher expression of CTLA-4 and PD-1 in T lymphocytes at parturition and in the prepartum period, which could indicate a relationship between these immune checkpoints and immunological tolerance during gestation in dairy cattle. In addition, a negative association between the expression of these immune checkpoints prepartum or at parturition and endometrial cytology at T20 and T30 was observed, indicating the negative implications of these immune response regulators in susceptibility to infections. This finding was further corroborated by the relationship between the serum concentration of haptoglobin and the expression of CTLA-4 and PD-1 by T lymphocytes. However, we did not observe a relationship between the indicators of negative energy balance, evaluated by the serum concentrations of BHB and NEFA, and the expression of the immune checkpoint markers studied. Thus, our findings represent an initial step that paves the way for the development of new therapeutic alternatives directed by the host with the objective of increasing the resistance of dairy cattle to infections in this critical period of life.

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Mastitis in the transition period: identification of potential blood markers

Cited by 5 publications

References 27 publications

Effect of mastitis on some hematological and biochemical parameters of Red Sokoto goats

Effect of mastitis on some hematological and biochemical parameters of Red Sokoto goats

Evaluation Effects of Coliform Mastitis on Hematological and Biochemical Parameters in Dairy Cows, in Kirkuk City, Iraq

Brief Research Report: Expression of PD-1 and CTLA-4 in T Lymphocytes and Their Relationship With the Periparturient Period and the Endometrial Cytology of Dairy Cows During the Postpartum Period

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