Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle

Abstract

Estrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided equally into two groups: ovariectomized (Ovx) and simulated ovariectomy (Sham). One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein (BSP), osteocalcin (OCC) and receptor activator of nuclear factor kappa-B ligand (RANKL). Results: Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups (p=0.049). No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups.

Degenerative Changes in the Temporomandibular Joint in Elderly Patients with Posterior Edentulous Maxilla and Mandibula According to Cone-Beam Computed Tomography Data.

“…These data are comparable with studies carried out earlier [6,10,18]. This feature is explained by the fact that women have hormonal effects of estrogen and prolactin, which can increase the degradation of cartilage and bone [19]. However, in our study, men have a greater total number of degenerative signs of TMJ.…”

The Influence of Alcohol Consumption in Conjunction with Sex Hormone Deficiency on Ca/P Ratio in Rats

“…This period of adaptation decreases a possible aversion to sudden dietary change, allowing greater acceptance of treatment. A similar adaptation protocol (the same timeframe and alcohol concentrations) has been described previously [ 20 ].…”

Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar rats

“…The dietary aspects of this study, as well the surgical procedures, were based on previously published studies which also analyzed the influence of alcohol consumption (20%) and estrogen deficiency in the periodontium of rats (Alonso, Souza, Balducci, & Rocha, 2016;Marchini et al, 2012Marchini et al, , 2014 or other rat tissues (de Deco et al, 2011;Lodi, Marchini, Santo, Rode, & Rocha, 2016;Salgado, Marchini, Tera, Rocha, & Marchini, 2015). However, it is important to highlight that the present study is not part of the abovementioned studies, as we used different animals and different analyses.…”