Resin-based sealer penetration into dentinal tubules after the use of 2% chlorhexidine gel and 17% EDTA: in vitro study

Abstract

Aim: To evaluate, by confocal laser scanning microscopy (CLSM), the influence of 17% EDTA final irrigation on the penetration of an endodontic resin-based sealer into dentinal tubules after use of 2% chlorhexidine gel. Methods: Forty extracted bovine incisors were instrumented according to the groups: G1 -root canal preparation with 2% chlorhexidine gel (n=10); G2 -root canal preparation with 2% chlorhexidine gel and final irrigation with 17% EDTA (n=10); G3 -root canal preparation with saline and final irrigation with 17% EDTA (n=10); G4 -root canal preparation with saline (n=10). The samples were filled with gutta-percha using AH Plus sealer with rhodamine B fluorescent dye. After seven days, the teeth were sectioned at the coronal, middle, and apical thirds and viewed under confocal microscope. The most representative area of penetration depth was measured in each group. Statistical significance for the sealer penetration area was determined among groups using one-way ANOVA followed by Tuckey test. For thirds comparison, in each group, data were statistically analyzed using Friedman test (p<0.05). Results:The maximum penetration was provided by G2 -2% chlorhexidine + EDTA (p=0.000). According to this criterion, no differences were found among the other groups and among thirds within the same group. Conclusions: Based on these results, the use of 17% EDTA should be indicated after root canal preparation with 2% chlorhexidine gel for smear layer removal, enhancing the AH Plus sealer penetration.

Penetrability of a new endodontic sealer: A confocal laser scanning microscopy evaluation

“…In this study, the final irrigation was performed with 17% EDTA solution with the aim to improve dentinal permeability, and high means of sealer penetration were observed for both groups. The results are in agreement with others authors who demonstrated that regardless of the root canal sealer, smear layer removal is mandatory to improve sealer penetration into dentinal tubules (Ordinola‐Zapata et al, ; Sabadin, Böttcher, Hoppe, Santos, & Grecca, ).…”

Irrigation in Endodontics: Polyhexanide Is a Promising Antibacterial Polymer in Root Canal Treatment

“…Third, the bacteria of endodontic infections exist not only in the form of planktonic suspensions, but also as part of biofilms, highly organized microbial communities united by an organic matrix mainly based on polysaccharides. Extracellular DNA (eDNA), enhancer RNA (eRNA), proteins, lipids, and other biomolecules are present in the matrix [ 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ].…”

Effect of different endodontic sealers on push-out bond strength of fiber posts

“…Some studies have stated that the penetration of resinbased sealers into dentinal tubules is higher than that of other sealers 11,25 . The degree of penetration of resin-based sealers depends on flow, surface tension, viscosity, chemical compounds, working time, setting time, and solubility of the sealers.…”

“…Endodontic sealers are among the factors that may have a negative influence on post retention inside the canal 2,10 . Endodontic sealers should seal the canal laterally and apically and have acceptable adaptation to the root canal dentin 11 . There is a growing interest in the utilization of resin-based sealers that bond simultaneously to core material and the canal wall, creating a monoblock 167 167 167 167 can adversely affect the bond strength between posts and root canal dentin 13 .…”