Protocol for reproduction and ploidy confirmation in Nodipecten nodosus (Linnaeus, 1758) by flow cytometry

Abstract

The production of scallops Nodipecten nodosus (LINNAEUS, 1758), a marine bivalve species, native and hermaphrodite from Brazil, has great potential in the state of Rio de Janeiro. The species has production protocols in the laboratory defined, ranging from reproduction and larviculture to the production of seeds (post-larvae), which are marketed to mariculturists.Throughout its cultivation, problems such as high mortality are encountered, yet without a reason (Landuci et al., 2021), and the application of biotechniques such as triploidy may bring improvements and zootechnical increments to production (Maldonado-Amparo et al., 2016). Thus, the present work was carried out with the objective of describing a protocol for species management in the laboratory, describing the methodology for broodstock selection, induced reproduction, gamete collection, ploidy analysis by cytometry, and some discussion possibilities for triploidization in the species.Faced with the aforementioned problems, there is a constant search for solutions, one of which is the production of triploid scallops. There are already reports of the advantages of triploids over diploids, such as higher growth and higher glycogen and protein content (Maldonado-Amparo et al., 2016).The procedures were carried out at the Mollusk