Efficacy of Purpureocillium lilacinum AUMC 10149 as biocontrol agent against root-knot nematode Meloidogyne incognita infecting tomato plant

Isaac, George Saad; El-Deriny, Marwa; Taha, Rania Gamal

doi:10.1590/1519-6984.253451

Cited by 4 publications

(1 citation statement)

References 23 publications

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“…Pretreatment of tomato seeds with Streptomyces hydrogenans DH-16 culture supernatant reduced nematode infestation and promoted tomato seedlings' growth (Sharma et al, 2020). Purpureocillium lilacinum AUMC 10149 is used to control the root-knot nematode M. incognita infestation of tomatoes (Isaac et al, 2021). Microorganisms, the natural enemies of nematodes, inhibit nematode disease through multiple pathways: the production of toxins, antibiotics, crystal proteins, and nematicidal substances (Zhang and Mo, 2006).…”

Section: Introductionmentioning

confidence: 99%

Nematicidal Activity of Burkholderia arboris J211 Against Meloidogyne incognita on Tobacco

Zhang

Ouyang

et al. 2022

Front. Microbiol.

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Root-knot nematode (Meloidogyne incognita) is the most widespread nematode affecting Solanaceae crops. Due to the lack of effective measures to control this nematode, its management can be achieved, using biocontrol agents. This study investigated in vitro efficacy of the antagonistic bacterial strain J211 isolated from tobacco rhizosphere soil against M. incognita, and further assessed its role in controlling nematodes, both in pot and field trials. Phylogenetic analysis of the 16S rRNA gene sequence of strain J211 assigned to Burkholderia arboris. Culture filtrates B. arboris J211 exhibited anematicidal activity against the second-stage juveniles (J2s) of M. incognita, with a 96.6% mortality after 24 h exposure. Inoculation of J211 in tobacco roots significantly reduced the root galling caused by M. incognita, both in pot and field trials. Meanwhile, plant growth-promoting (PGP) traits results showed that J211 had outstanding IAA-producing activity, and the IAA production reached 66.60 mg L−1. In the field study, B. arboris J211 also promoted tobacco growth and increase flue-cured tobacco yield by 8.7–24.3%. Overall, B. arboris J211 as a high-yielding IAA nematicidal strain effectively controlled M. incognita and improved tobacco yield making it a promising alternative bionematocide.

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Section: Introductionmentioning

confidence: 99%

Nematicidal Activity of Burkholderia arboris J211 Against Meloidogyne incognita on Tobacco

Zhang

Ouyang

et al. 2022

Front. Microbiol.

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Purpureocillium lilacinum strain AUMC 10620 as a biocontrol agent against the citrus nematode Tylenchulus semipenetrans under laboratory and field conditions

et al. 2023

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Three concentrations (1.25, 2.5, and 5 × 107 spores ml−1) (of the biocontrol fungus Purpureocillium lilacinum (strain AUMC 10620) were tested on citrus nematode Tylenchulus semipenetrans under in vitro and field conditions. Larvae and eggs were exposed to the fungal spores in vitro for 24, 48, and 72 h, and the findings were recorded at each time point. These results were compared with the application of the nematicide abamectin. Strain AUMC 10620 effectively reduced larval activity and egg hatching of T. semipenetrans under laboratory conditions. The highest concentration (5× 107 spores ml−1) of P. lilacinum, resulted in 89.01% immobility in the larvae, compared to abamectin, which resulted in 65.93% immobility after 48 h of exposure. These percentages of immobility were increased after 72 h of exposure (100 and 85.09%) when P. lilacinum at a concentration of 5 × 107 spores ml−1 and abamectin were used, respectively. On the other hand, the two other P. lilacinum concentrations (1.25, and 2.5 × 107 spores ml−1) affected the T. semipenetrans larvae to a lesser extent. The highest fungal concentration 5× 107 spores ml−1 inhibited the hatching of T. semipenetrans eggs in vitro with 71.34, 80, and 86.67% after 24, 48 and 72 h of treatment compared to the abamectin treatment which showed 76.67, 78, and 87% after the abovementioned periods, respectively. In addition, the application of P. lilacinum (5 × 107 spores ml−1) or abamectin under field conditions significantly (P < 0.05) reduced the population of the major nematode species (T. semipenetrans, Tylenchorhynchus spp., Helicotylenchus spp., and Pratylenchus spp.) infesting citrus after one, two, and three weeks of treatment compared to the control treatment but with no significant (P > 0.05) differences between the two treatments. Three weeks after the field application, the percentage of nematode reduction was significantly (P < 0.05) smaller than the control treatment at concentrations of 5, 2.5, and 1.25 × 107 spores ml−1, respectively, by 78.42, 64.03, and 58.35%. It is evident from these results that the application of P. lilacinum strain (AUMC 10620) can be used in integrated pest management programs to control nematodes infesting citrus trees. Graphical abstract

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Morphological and molecular identification of the local entomopathogenic fungus Purpureocillium lilacinum and its pathogenicity against the larvae of superworm Zophobas morio (Coleoptera: Tenebrionidae)

Sebumpan,

Bataclan,

Sumaya

2023

Archives of Phytopathology and Plant Protection

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Efficacy of Purpureocillium lilacinum AUMC 10149 as biocontrol agent against root-knot nematode Meloidogyne incognita infecting tomato plant

Cited by 4 publications

References 23 publications

Nematicidal Activity of Burkholderia arboris J211 Against Meloidogyne incognita on Tobacco

Nematicidal Activity of Burkholderia arboris J211 Against Meloidogyne incognita on Tobacco

Purpureocillium lilacinum strain AUMC 10620 as a biocontrol agent against the citrus nematode Tylenchulus semipenetrans under laboratory and field conditions

Morphological and molecular identification of the local entomopathogenic fungus Purpureocillium lilacinum and its pathogenicity against the larvae of superworm Zophobas morio (Coleoptera: Tenebrionidae)

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