Spectral analysis, in vitro cytotoxicity and antibacterial studies of bioactive principles from the leaves of Conocarpus lancifolius, a common tree of Jazan, Saudi Arabia

Abstract

The objective of the present study was to analyse the bioactive compounds of the leaves of Conocarpus lancifolius (C. lancifolius). The GC-MS analysis of the hot methanolic extract of the leaves (HMEL) of C. lancifolius exhibited the bioactive compounds such as 1-(3-Methoxy-2-nitrobenzyl) iso quinoline, morphin-4-ol-6,7-dione, 1-bromo-N-methyl-, phytol, hexadecanoic acid, 2,3-dihydroxypropyl ester, 2,2':4',2”-terthiophene, ethyl iso-allocholate, caryophyllene oxide, campesterol, epiglobulol, cholestan-3-ol, 2-methylene-, (3á,5à)-, dasycarpidan-1-methanol, acetate (ester) and oleic acid, eicosyl ester. The FT-IR analysis of HMEL of C. lancifolius showed a unique peak at 3184, 2413, 1657 cm-1 representing coumaric acid, chlorogenic acid and ferulic acid. The HMEL of C. lancifolius was actively inhibiting the proliferation of breast cancer cells MCF-7 ATCC at the concentration of 72.66 ± 8.21 µg/ml as IC50 value. The HMEL of C. lancifolius also revealed a good spectrum of activity against Gram-positive and Gram-negative bacterial cultures screened in this work. The activity observed has shown more or less similar effects against screened bacteria. However, the magnitude of potentiality was significantly lesser compared to standard ciprofloxacin disc at p< 0.001 level (99% confidence intervals). Furthermore, the study demonstrating the bioactive compounds can be isolated from the leaves of C. lancifolius.

Enhancing systemic resistance in faba bean (Vicia faba L.) to Bean yellow mosaic virus via soil application and foliar spray of nitrogen-fixing Rhizobium leguminosarum bv. viciae strain 33504-Alex1

“…Hexadecanoic acid 2,3-dihydroxypropyl ester (known as palmitate) is a biologically active fatty acid with reported antitumor activity against human leukemia cells, with no side effects or cytotoxicity to normal human dermal fibroblast ( Al-Wahaibi et al, 2020 ). It was reported that the broad-spectrum antimicrobial activity of Conocarpus lancifolius leaf extract and its antitumor activity against breast cancer might be attributed to the presence of hexadecanoic acid, 2,3-dihydroxypropyl ester ( Moni et al, 2021 ). Furthermore, oleic acids are biologically active monounsaturated fatty acids with reported antibacterial, antioxidant, and antiviral activities ( Dilika et al, 2000 ; Wei et al, 2016 ; Zhao et al, 2017 ).…”

Azadirachta indica A. Juss Fruit Mesocarp and Epicarp Extracts Induce Antimicrobial and Antiproliferative Effects against Prostate (PC-3), Breast (MCF-7), and Colorectal Adenocarcinoma (Caco-2) Cancer Cell Lines through Upregulation of Proapoptotic Genes

“…Most of these have been reported as potent bioactive components. The spectrum of antibacterial activity was ascribed by several authors to the presence of hexadecanoic acid, 2,3-dihydroxypropyl ester [ 34 ] and 1,2-benzenedicarboxylic acid, bis(2-ethylhexyl) ester [ 35 ]. The other components include octadecanoic acid, 2,3-dihydroxypropyl ester (Stearin), a glycerol derivative, which has anticancer and antimicrobial activities [ 36 ], and 9,12-Octadecadienoic acid (Z,Z)-, which is a fatty acid reported to have anti-inflammatory, cancer-preventive, and antiprostatitic properties [ 30 ].…”

In vitro antifungal and antibacterial potentials of organic extracts of Avicennia marina collected from Rabigh Lagoon, Red Sea Coasts in Saudi Arabia

“…Previous research has found that distinct extracts of multiple medicinal plants obtained from various locations around the Kingdom of Saudi Arabia and belonging to different Botanic families exhibit interesting antibacterial and antifungal properties. Among them species from Boraginaceae, Asteraceae, Polygonaceae, Rhamnaceae, Resedaceae, Euphorbiaceae Combretaceae and Acanthaceae (Moni et al, 2023;Aly and Bafeel, 2010;Alamri and Moustafa, 2012;Shahat et al, 2017;Okla et al, 2021).…”