Murinometric measurements and retroperitoneal adipose tissue in young rats exposed to the high-fat diet: Is there correlation?

Abstract

Aim This study aimed to verify the correlation between murine measurements and retroperitoneal adipose tissue in rats exposed to the high-fat diet. Material and methods: Wistar male adult rats, descendants of mothers who consumed a high-fat diet during pregnancy and lactation and fed the same diet after weaning were used. At 60 days of life, body weight, longitudinal axis and waist circumference (WC) were measured. The Body Mass Index (BMI) and the Lee Index were calculated for a posterior analysis of the correlation with the amount of retroperitoneal adipose tissue dissected on the same day. For analysis of the data, the Pearson correlation test was used, considering statistical significance for p <0.05. Results: Body weight had a weak correlation (r= 0.31; p= 0.38) with retroperitoneal adipose tissue. While the longitudinal correlated moderately and negative (r= -0.40; p= 0.25). Abdominal circumference (r= 0.62; p= 0.05), body mass index (r= 0.61; p= 0.03) and Lee (r= 0.69; p= 0.03) correlated moderately and positively with adipose tissue. Conclusion: Among the measured murine measurements, weight and longitudinal axis were not good indicators to represent accumulation of retroperitoneal adipose tissue in rats. However, Lee's index seems to be the best murine marker to diagnose the accumulation of retroperitoneal fat. BMI, CA and Lee index were murine parameters with higher correlation.

Effect of virgin coconut oil on body weight, white fat depots, and biochemical and morphological parameters in mice fed standard or high-fat diets

“…However, animals fed HFDs did not exhibit a significant increase in the final BW compared with animals fed SDs. According to Macêdo et al , 31 an HFD is able to promote the accumulation of retroperitoneal fat, and the Lee index seems to be the best marker to diagnose retroperitoneal fat accumulation in mice. Moreover, Pomar et al 32 demonstrated that the offspring of rats fed an HFD during lactation, despite having a greater amount of adipose tissue, had a lower BW as a result of a lower amount of lean mass.…”

Exposição a dieta hiperlipídica no período perinatal e pós desmame aumenta risco cardiometabólico e altera histomorfometria aórtica em ratos jovens

“…O índice de massa corporal é calculado pela relação entre o peso e o comprimento do rato e tem sido utilizado como indicador de adiposidade em ratos (Macêdo et al 2020). No entanto, no presente estudo apenas a exposição perinatal a dieta hiperlipídica foi capaz alterar esse indicador.…”

“…O peso corporal e o comprimento dos filhotes foram aferidos no primeiro e último dia de experimento. Em seguida foi calculado o IMC (peso(g)/ comprimento naso -anal (cm) 2 (MACÊDO et al 2020;NOVELLI et al, 2007) . No 60º dia de vida, após jejum de 12 horas, foi realizada eutanásia e o sangue dos animais foi coletado para análise dos parâmetros bioquímicos.…”