Proposed method for agglutinating antibody titer analysis and its use as indicator of acquired immunity in pacu, Piaractus mesopotamicus

Biller-Takahashi, JD; Montassier, Hélio José; Takahashi, Leonardo Susumu; Urbinati, Elisabeth Criscuolo

doi:10.1590/1519-6984.21312

Cited by 15 publications

(15 citation statements)

References 31 publications

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“…Testing of antibody titer was performed on a 96-wells microplate-based on a method referred to Takahashi et al (2014). Measurement of antibody titer was performed by taking 25 μL of PBS solutions and put into microplate at holes 1 to 12, then inserted blood serum in the hole 1 as much as 25 μL.…”

Section: Antibody Testingmentioning

confidence: 99%

Efficacy of bivalent vaccine against black body syndrome (BBS) of barramundi Lates calcalifer B.

Nugrahawati

Nuryati

Sukenda

et al. 2019

Jurnal Akuakultur Indonesia

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Black body syndrome causes mass mortality in barramundi (Lates calcarifer) in Asia mariculture. This study aimed to evaluate the efficacy of bivalent vaccine derived from local isolate for black body syndrome (BBS) prevention on the Barramundi. The bacteria used in the bivalent vaccine were Pseudomonas stutzeri and Vibrio harveyi in a ratio of 50:50. Barramundi, 5‒6 cm in length, was intraperitoneally injected with bivalent vaccine and phosphate buffer saline (PBS). After a 21-day vaccination, fish was injected with pathogenic bacteria P. stutzeri, V. harveyi, the combination of P. stutzeri and V. harveyi with a dose of bacteria 107 CFU/fish. We observed relative percent survival (RPS), mortality, blood profile, antibody level, lysozyme activity, and histopathology of vaccinated fish. The result showed that vaccinated fish had higher antibody levels and lysozyme activity than control treatment (P>0.05). Vaccinated fish had RPS of 80.00%, 64.29%, 57.69%, after challenged test with P. stutzeri, V. harveyi, and combination of P. stutzeri and V. harveyi, respectively. Hemoglobin and hematocrit were not significantly different (P>0.05). However, the erythrocytes, leucocytes, and phagocytic activity were higher compared to there were higher erythrocytes, leucocytes, and phagocytic activity compared to control (P<0.05). As well as antibody level and lysozyme activity of vaccinated fish higher than control (P<0.05). In conclusion, the bivalent vaccine of P. stutzeri and V. harveyi could protect barramundi seed from BBS infection. Keywords: Lates calcalifer, bivalent vaccine, local isolate, Pseudomonas stutzeri, Vibrio harveyi ABSTRAK Black body syndrome menyebabkan kematian masal pada ikan kakap putih budidaya air laut di wilayah Asia. Tujuan dari penelitian ini adalah untuk menguji efikasi vaksin bivalen untuk penanggulangan black body syndrome pada benih ikan kakap putih. Bakteri yang digunakan untuk membuat vaksin bivalen yaitu Pseudomonas stutzeri dan Vibrio harveyi dengan rasio 50:50. Ikan kakap putih berukuran 5‒6 cm diinjeksi vaksin bivalen dan phosphat buffer saline secara intraperitoneal. Setelah 21 hari pemeliharaan, benih diuji tantang dengan bakteri patogen P. stutzeri, V. harveyi, campuran P. stutzeri dan V. harveyi dengan kepadatan bakteri sebesar 107 CFU/ikan. Parameter yang diamati meliputi relative percent survival (RPS), gambaran darah, titer antibodi, aktifitas lisosim, dan gambaran histopatologi benih kakap putih. Hasil penelitian menunjukkan bahwa ikan yang divaksin memiliki nilai titer antibodi dan aktifitas lisosim lebih tinggi dibandingkan kontrol (P<0.05). Ikan yang divaksin memilki RPS 80.00%, 64.29%, 57.69%, setelah diuji tantang dengan P. stutzeri, V. harveyi, campuran P. stutzeri dan V. harveyi. Nilai hemoglobin dan hematokrit tidak berbeda nyata (P>0.05). Titer antibodi dan aktifitas lisosim ikan yang divaksin lebih tinggi dari kontrol (P<0.05). Vaksin bivalen P. stutzeri dan V. harveyi dapat melindungi benih ikan kakap putih dari infeksi BBS. Kata kunci : Lates calcalifer, vaksin bivalen, Pseudomonas stutzeri, Vibrio harveyi

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Section: Antibody Testingmentioning

confidence: 99%

Efficacy of bivalent vaccine against black body syndrome (BBS) of barramundi Lates calcalifer B.

Nugrahawati

Nuryati

Sukenda

et al. 2019

Jurnal Akuakultur Indonesia

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“…However, its farming has been facing disease threats and little information is available on the use of immunostimulants or the effects of bacterial infection in general Brazilian aquaculture (Garcia et al, 2007;Martins et al, 2009a;Biller-Takahashi et al, 2014). In the light of these facts, the purpose of this study was to evaluate the effect of oral administration of β-glucan on disease resistance of pacu experimentally infected with A. hydrophila.…”

Section: Introductionmentioning

confidence: 99%

Disease resistance of pacu Piaractus mesopotamicus (Holmberg, 1887) fed with β-glucan

et al. 2014

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Effects of β-glucan on innate immune responses and survival were studied in pacu experimentally infected with Aeromonas hydrophila. Fish fed diets containing 0, 0.1% and 1% β-glucan were injected with A. hydrophila. β-glucan enhanced fish survival in both treated groups (26.7% and 21.2% of the control, respectively). Leukocyte respiratory burst and alternative complement pathway activities were elevated after bacterial challenge regardless the β-glucan concentration. Lysozyme activity was higher after infection and showed a gradual increase as β-glucan concentration increased. A significant elevation in WBC count was observed either after bacterial challenge or by influence of β-glucan separately. The same response was observed in the number of thrombocytes, lymphocytes, eosinophils, LG-PAS positive cell and monocytes. It can be concluded that feeding pacu with β-glucan can increase protection against A. hydrophila, due to changes in non-specific immune responses.Keywords: immunology, complement system, innate immunity, lysozyme, respiratory burst activity of leukocytes. Resistência à doença em pacu, Piaractus mesopotamicus(Holmberg, 1887), alimentados com β-glucana ResumoOs efeitos da β-glucana sobre as respostas imunes inatas e a sobrevivência foram estudados em pacu experimentalmente infectado com Aeromonas hydrophila. Peixes alimentados com dietas contendo 0,1% e 1% de β-glucana foram injetados com 1 x 10 8 CFU de A. hydrophila após 7 dias de alimentação. A sobrevivência de peixes foi maior nos dois grupos tratados em comparação ao grupo controle (26,7% e 21,2%, respectivamente). A atividade respiratória de leucócitos e a atividade hemolítica do complemento -via alternativa estavam elevadas após desafio bacteriano independentemente da concentração de β-glucana. A atividade de lisozima foi maior após a infecção e mostrou um aumento gradual de acordo com a concentração do imunoestimulante. Observou-se um aumento significativo na contagem de leucócitos totais após o desafio bacteriano e influência de β-glucana. A mesma resposta foi observada para trombócitos, linfócitos, eosinófilos, leucócito PAS positivo e monócitos. Com exceção de neutrófilos, que diminuíram frente ao mais alto nível do imunoestimulante e não se alteraram após a infecção, as outras células aumentaram após a exposição à A. hydrophila. A β-glucana não afetou os níveis de proteína total do soro, que aumentaram após o desafio bacteriano. Conclui-se que a administração de β-glucana em pacu pode aumentar a proteção contra A. hydrophila, por alterações nas respostas imunes de não-específicas.Palavras-chave: imunologia, sistema complemento, imunidade inata, lisozima, atividade respiratória de leucócitos.

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“…Vet. Zootec., v.65, n.6, p. [1745][1746][1747][1748][1749][1750][1751]2013 are responsible for detecting the pathogenic agent, and can be found in the cell membrane of T lymphocytes (TCR -T cell receptor) and B lymphocytes (BCR, B cell receptor, also called a membrane immunoglobulin) or in serum as free antibody (Holland and Lambris, 2002;Abbas and Litchman, 2004;Boltaña et al, 2011).…”

mentioning

confidence: 99%

“…Regarding phagocytes, neutrophils and macrophages are very important because of their large quantities of lysosomal enzymes and the production of reactive oxygen species responsible for the destruction of invading bacteria (Ellis, 1999, Biller et al, 2012.…”

mentioning

confidence: 99%

“…The standardization of techniques in order to assess the indicators of fish immunity is highly valuable for National aquaculture survey. Regarding pacu, Piaractus mesopotamicus (Holmberg, 1887), it is one of the most important fish in national aquaculture, however there is a lack of studies on its immune responses (Abreu et al, 2009;Martins et al, 2009;Sado et al, 2010;Biller et al, 2012;Biller et al, 2013). In addition, the pathogenic agent used in the challenge is an important bacterium that causes severe disease outbreaks in Brazilian aquaculture and worldwide (Biller et al, 2014).…”

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confidence: 99%

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Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)

Biller-Takahashi

Takahashi

Pilarski³

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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The immune system of teleost fish has mechanisms responsible for the defense against bacteria through protective proteins in several tissues. The protein action can be evaluated by serum bactericidal activity and this is an important tool to analyze the immune system. Pacu, Piaractus mesopotamicus, is one of the most important fish in national aquaculture. However there is a lack of studies on its immune responses. In order to standardize and assess the accuracy of the serum bactericidal activity assay, fish were briefly challenged with Aeromonas hydrophila and sampled one week after the challenge. The bacterial infection increased the concentration of protective proteins, resulting in a decrease of colony-forming unit values expressed as well as an enhanced serum bactericidal activity. The protocol showed a reliable assay, appropriate to determine the serum bactericidal activity of pacu in the present experimental conditions.

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Proposed method for agglutinating antibody titer analysis and its use as indicator of acquired immunity in pacu, Piaractus mesopotamicus

Cited by 15 publications

References 31 publications

Efficacy of bivalent vaccine against black body syndrome (BBS) of barramundi Lates calcalifer B.

Efficacy of bivalent vaccine against black body syndrome (BBS) of barramundi Lates calcalifer B.

Disease resistance of pacu Piaractus mesopotamicus (Holmberg, 1887) fed with β-glucan

Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)

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