Alternative substrates for conidiogenesis of the entomopathogenic fungus Beauveria bassiana (Bals) Vuillemin (Deuteromycotina: Hyphomycetes)

Abstract

<jats:p>Abstract Beauveria bassiana is a promising fungus for the biological control of insect pests. The growing costs of conidia production have raised the need to ascertain the efficiency of some low cost substrates. The aim of this study was to analyze the potential use of different raw substrates without nutritional supplement for B. bassiana conidiogenesis. Growth and sporulation were evaluated using 30 g of substrate and 0.3 μL of a conidia suspension (1 x 106 conidia/mL). After 10 days of incubation (70 ± 10% humidity and temperature (T) = 29 ± 1 °C), rice (2.00 x 106 conidia/g substrate), algaroba (2.36 x 106 conidia/g), malt A (1.22 x 106 conidia/g) and malt B (1.75 x 106 conidia/g) showed the highest levels of conidia production. The resulting conidia showed insecticidal activity higher than 80% on coconut termites. These new raw substrates may represent viable alternatives for the production of entomopathogenic fungi for use in the biological control of various insect pests.</jats:p>

Caracterização morfofisiológica de fungos entomopatogênicos para o controle biológico de <i>Oncideres impluviata</i>

“…Verificando a produção de conídios no meio de cultura completo, Guimarães et al (2020) observaram, após 10 dias de crescimento do fungo, que Beauveria bassiana foi mais produtiva nos substratos arroz, malte A, malte B e fibra de algaroba, não diferindo estatisticamente entre si, com rendimentos de 2,00 x 10 6 ; 1,22 x 10 6 ; 1,75 x 10 6 e 2,36 x 10 6 conídios/grama, respectivamente. A esporulação variou estatisticamente nos substratos bagaço da cana-de-açúcar, sementes e fibras da acerola (0,85 x 10 6 ; 0,56 x 10 6 e 0,54 x 10 6 , respectivamente).…”

Effect of Agrochemicals Used in the Cultivation of Soybean and Irrigated Rice on Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

“…The use of the fungi B. bassiana and M. anisopliae is based particularly on the high genetic variability, their broad spectrum of hosts and the need of ingestion by the insect pests. Generally, the infection they cause is through germ tube growth and introduction of extracellular proteases on the cuticle of the insect, this differentiates them from bacteria and viruses, whose infection occurs only by ingestion (Alves et al, 1998;Paiva-Guimarães et al, 2019).…”