Aquaculture biological waste as culture medium to cultivation of Ankistrodesmus gracilis (Reinsch) Korshikov

Abstract

Current study investigated the effectiveness of different macrophytes as culture media for Ankistrodesmus gracilis in laboratory conditions. Significant difference (p < 0.05) was reported in cell density with regard to conventional culture medium (CHU 12 ) and macrophytes culture media. Mean cell density in NPK, Eichhornia crassipes and E. azurea media was higher (p < 0.05) than in conventional culture medium. Chlorophyll-a was higher than 1 g.L (P. cordata medium) and 1.7 g.L -1 (CHU 12 medium) for ammonium, and between 0.8 g.L -1 (CHU 12 medium) and 1.9 g.L -1 (T. domingensis medium) for total phosphorus. Results revealed inorganic fertilizer and macrophytes combined with vitamins may be effective as culture media and strongly supports the growth of Ankistrodesmus gracilis, since cell density and biochemical composition are similar to or higher than conventional culture medium (CHU 12 ). Macrophyte is a tool for aquaculture since biological wastes may be used with nutrients to improve the cultivation of microalgae.Keywords: aquatic plants, culture media, microalgae growth, biochemical composition. Resíduos biológicos de aquicultura como meio de cultura para o cultivo de Ankistrodesmus gracilis (Reinsch) KorshikovResumo O presente estudo investigou a eficácia na utilização de diferentes macrófitas como meio de cultura para Ankistrodesmus gracilis em condições laboratoriais. Foi observada diferença significativa (p < 0,05) entre a densidade celular em relação aos meios de cultura convencional (CHU 12 ) e de macrófitas. Os meios de cultura com Eichhornia crassipes, E. azurea e NPK apresentaram densidade celular maiores (p < 0,05) que o meio de cultura convencional. Os teores de clorofila-a foram superiores a 1 g.L -1 , exceto nos meios de cultura CHU 12 (0.7 ± 0.4 g.L -1 ) e de T. domingensis (0.7 ± 0.3 g.L -1 ). O nitrato diminuiu acentuadamente a partir do 7º dia de experimento. Dentre os nutrientes, amônia e o fósforo total foram os mais elevados nos meios de cultura, variando entre 0.4 g.L -1 (meio de P. cordata) a 1,7 g.L -1 (meio CHU 12 ) para amônia e, entre 0.8 g.L -1 (meio CHU 12 ) e 1,9 gL -1 (meio de T. domingensis) para o fósforo total. Os resultados do presente estudo mostraram que o uso de fertilizantes inorgânicos e macrófitas, combinados com vitaminas, pode ser eficaz como meio de cultura no crescimento de Ankistrodesmus gracilis, uma vez que a densidade celular e a composição bioquímica foram semelhantes ou superiores ao meio de cultura convencional (CHU 12 ). As macrófitas são ferramentas a serem adotadas na aquicultura, desde que os resíduos biológicos podem ser usados como nutrientes para melhorar o cultivo de microalgas.Palavras-chave: plantas aquáticas, meio de cultura, crescimento de microalga, composição bioquímica.

Microalgae Ankistrodesmus gracilis as feed ingredient for ornamental fish Xiphophorus maculatus

“…Macrophytes Azolla caroliniana (AAC) and Lemna minor (ALM) were used as culture media. Culture medium with different macrophytes has already been discussed by Sipaúba-Tavares et al (2009, 2018. The macrophytes were chosen due to their great availability and abundance on the site.…”

“…Fish may be maintained and developed by live feed through large-scale production of microalgae at high costs, especially with regard to culture media. Several research works have underscored the use of alternative culture media, such as inorganic fertilizers (El-Nabris 2012; Ramaraj et al 2015) or the employment of organic wastes retrieved from aquaculture, such as macrophytes (Sipaúba-Tavares et al 2009, 2018.…”

A 1232 bp upstream sequence of glutamine synthetase 1b from Eichhornia crassipes is a root-preferential promoter sequence

“…Also, it is regarded as a valuable resource with several unique properties, and previous studies have reported that E. crassipes had high absorption efficiency of nitrogen (N), phosphorus (P) and heavy metals [4][5][6]. So it was used in the bioremediation of pollutants present in water [7,8]. Furthermore, E. crassipes served as a good economic raw material for the production of feeding and energy fuel [9][10][11].…”