Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profile

Abstract

Synthetic polyploids are key breeding materials for watermelon. Compared with diploid watermelon, the tetraploid watermelon often exhibit wide phenotypic differences and differential gene expression. Digital gene expression (DGE) profile technique was performed in this study to present gene expression patterns in an autotetraploid and its progenitor diploid watermelon, and deferentially expressed genes (DEGs) related to the abiotic and biotic stress were also addressed. Altogether, 4,985 DEGs were obtained in the autotetraploid against its progenitor diploid, and 66.02% DEGs is up-regulated. GO analysis shows that these DEGs mainly distributed in 'metabolic process', 'cell' and 'catalytic activity'. KEGG analysis revealed that these DEGs mainly cover 'metabolic pathways', 'secondary metabolites' and 'ribosome'. Moreover, 134 tolerance related DEGs were identified which cover osmotic adjustment substance, protective enzymes/protein, signaling proteins and pathogenesis-related proteins. This study present the differential expression of stress related genes and global gene expression patterns at background level in autotetraploid watermelons. These new evidences could supplement the molecular theoretical basis for the better resistance after the genome doubling in the gourd family.

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

“…Taking into account that the species is an aromatic shrub with pharmacological and economic applications, those synthetic plants may open a new scenario for manipulating the genome, regarding the gene expression profile or the production of secondary metabolites of commercial interest (Castro et al, 2018;Long et al, 2019).…”

Polyploidy in tissues of plants in vitro of grape somaclones

“…Achieving the undoubted advantages that polyploid organisms possess is the aim of many breeders who use induced polyploidy and natural polyploids to create new plant varieties [3]. Polyploid forms of plants are characterized by increased organ sizes compared to diploid varieties, buffering of harmful mutations, increased heterozygosity and heterosis [3][4][5]. Polyploidy allows obtaining seedless varieties due to errors that occur in meiosis; at the same time, doubling the genome in a sterile hybrid allows restoring its fertility [3,6].…”