Wound-Healing potential of Sebastiania hispida (Mart.) Pax (Euphorbiaceae) ointment compared to low power laser in rats

Abstract

Impaired wound healing represents a serious complication in some pathologies and the use of plant extracts has proved to improve tissue repair. The objectives of this study were to evaluate the healing potential of the ointment of Sebastiana hispida compared with Aluminum-Gallium Indium-Phosphide Laser (InGaAlP) in surgically induced wounds in rats and to perform the phytochemical analysis. The phytochemical analysis was performed in the classic way and also by HPLC. A controlled study was developed with 80 rats (200-250 g) in which a linear excision was performed in the dorsal region after shaving, measuring 2 cm × 1 cm (epidermis and dermis) exposing the muscle fascia. The rats were randomly divided into four groups of twenty animals each. The experimental groups (n = 5) were G1 (Saline); G2 (crude methanol plant extract 2% + Carbopol Gel 98%); G3 (crude methanol plant extract 2% + lanolin/vaseline) and G4 (laser). The incision healing processes were monitored during 3, 7, 14 and until 21 days after excision. The histologic parameters evaluated were Collagen fiber types, microscopic examination and neovascularization. There was a significant increase in the deposition of collagen fibers, as evidenced by a better organized epithelial tissue, keratinized and showing greater proliferation of new blood vessels in the inflammatory phase in the group treated with both the extract and laser. The results were correlated to the phenolic derivatives found after qualitative and quantitative analysis. These compounds were considered responsible for the healing process. The topical treatment with S. hispida leaves, in the two different formulations, was more effective than the application of the laser (Ingan ALP) 660 nm in the model used.Keywords: laser therapy, tissue repair, inflammatory cells, medicinal plants. Potencial cicatrizante da pomada de Sebastiania hispida (Mart.) Pax (Euphorbiaceae) comparado ao laser de baixa potência ResumoA cicatrização deficiente representa uma complicação grave em algumas patologias e o uso de extratos de plantas tem demonstrado melhora no processo de reparação tecidual. O objetivo deste estudo foi avaliar o potencial cicatrizante da pomada de Sebastiania hispida comparado ao Laser Indio Phosphide-Gallium-Aluminum (InGaAlP) em feridas cutâneas induzidas cirurgicamente em ratos e realizar analise fitoquímica. A análise fitoquímica foi via clássica e por CLAE. O experimento foi desenvolvido com 80 ratos (200-250 g), divididos aleatoriamente em quatro grupos (n = 5): G1 (salina); G2 (extrato bruto metanólico 2% + Carbopol Gel 98%); G3 (extrato bruto metanólico 2% + lanolina/ vaselina) e G4 (laser). Após a tricotomia da região dorsal realizou uma excisão linear, medindo 2 cm × 1 cm (epiderme e derme), expondo a fáscia muscular. Em sequência os tratamentos e o monitoramento do processo de cicatrização das feridas cirúrgicas ocorreu nos tempos de: 3, 7, 14 e até 21 dias após a excisão. Os parâmetros histológicos avaliadosBraz.

Efficacy of application of Equisetum pyramidale Goldm. hydrogel for tissue restoration of induced skin lesions in Wistar rats

“…According to Lima et al (2009) and Rizzi et al (2017), for a complete and effective healing of the tissue it is necessary a progressive deposition and remodeling of collagen fibers in association with controlled inflammatory processes, which is also observed in our findings. In addition, the effects of the hydrogel vehicle were satisfactory when compared to the control group, since plastic compression and permanence of the extract in the lesion could have occurred.…”

Benefits of Sebastiania hispida (Euphorbiaceae) extract and photobiomodulation therapy as potentially adjunctive strategies to be explored against snake envenoming

“…1A). These results have great similarity in terms of the chemical constituent to the specimens of S. hispida collected in other regions of the Brazilian Cerrado [24,25], except for the saponins, anthraquinones, steroids and reducing sugars (Fig. 1A).…”

“…On the other hand, the chemical arsenal of constituents in plants makes important therapeutic allies to neutralize snake venom effects [69][70][71][72]. In this sense, S. hispida richness in flavonoids and phenolic compounds showed wound healing activity [24], likely based on its antibacterial activity [25]. In the present study model of B. moojeni-induced myonecrosis the partial but effective efficacy of S. hispida hydro-MeOH extract might rely on secondary metabolites, flavonoids and tannins, which demonstrated their anti-inflammatory and antioxidant properties [73,74].…”

“…Although the chemical composition of plants is strongly influenced by biotic and abiotic factors [19,49], it was observed from the phytochemical analysis of S. hispida collected in Campo Grande (MS state) [24,25] and that of our study collected in Aquidauna city, that there were no changes in the substances content of phenolic and flavonoid compounds, phytomolecules with potential applications such as antimicrobials, antioxidants and other functional activities [23][24][25], and which may be related to the therapeutic effect evidenced presently.…”

“…Sebastiania hispida (Mart.) Pax (Euphorbiaceae) is a native species of the Cerrado bioma characteristic of semi-flooded fields at the Brazilian Pantanal and that has been described as possessing a significant anti-inflammatory potential [23][24][25].…”