Distribution and genetic diversity of the human polyomaviruses JC and BK in surface water and sewage treatment plant during 2009 in Porto Alegre, Southern Brazil

Comerlato, Juliana; Campos, Fabrício Souza; Souza-Arantes, T.; Roos-Kulmann, M. I.; Oliveira, Martha Trindade; Spilki, Fernando Rosado; Frazzon, Ana Paula Guedes; Roehe, Paulo Michel; Franco, Ana Cláudia

doi:10.1590/1519-6984.15415

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…The presence of the JCPyV in water from the metropolitan area of Berlin was also demonstrated and agres to that JCPyV is the most prevalent polyomavirus in natural environments due to contamination with human wastes [46]. In agreement to our results, it was recently described the presence of JCPyV and BKPyV in water samples from a nearby region in Porto Alegre [47].…”

Section: Discussionsupporting

confidence: 92%

Occurrence of Polyomaviruses in Recreational Freshwaters from Southern Brazil

Urbano¹,

Girardi

Leal³

et al. 2019

BJSTR

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There is little information about the transmission pathways of polyomaviruses. Since many of them are regularly found in urine and feces, the possibility that the waterborne route could transmit these viruses has not been explored. The aim of this study was to evaluate the presence of human polyomaviruses JCPyV, BKPyV, MCPyV, STLPyV and TSPyV along the Belo River (Caxias do Sul-Rio Grande do Sul-RS, Brazil). Four points were investigated, with 13 monthly samplings (March/2015 -March/2016). A total of 52 samples were concentrated by ultracentrifugation, total genomic material was extracted using commercial extraction kit and human polyomaviruses were investigated through real time polymerase chain reaction. A total of 32/52 (61.5%) positive samples were found. Only JCPyV and BKPyV were identified, and they were present in all sampling points. The average viral load of JCPyV was 7E+05 copies/mL and of BKPyV was 5E+04 copies/mL after particle concentration process. Finding such polyomaviruses in this environment suggests contamination through human waste and reinforce the notion that fecal-oral route may represent an important transmission mode for these viruses.(STLPyV). It was detected in human feces by pyrosequencing8, but so far there is no association of STLPyV with diseases.

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Section: Discussionsupporting

confidence: 92%

Occurrence of Polyomaviruses in Recreational Freshwaters from Southern Brazil

Urbano¹,

Girardi

Leal³

et al. 2019

BJSTR

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“…Among the identified viruses, the virus with the highest initial concentration in all experiments was JC polyomavirus, a human virus with a circular dsDNA genome, widely present in urban sewages [47][48][49], due to its continuous excretion by infected individuals [50]. The pathogenicity and virulence of this type of virus is mostly associated with immunocompromised states, such as those observed in patients with advanced HIV infection or leukemia.…”

Section: Virus Removal From Municipal Effluentmentioning

confidence: 99%

Removal of Enteric Pathogens from Real Wastewater Using Single and Catalytic Ozonation

Gomes

Frasson

Quinta‐Ferreira

et al. 2019

Water

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Water scarcity is one of the main problems of this century. Water reclamation appears as an alternative due to the reuse of treated wastewater. Therefore, effluents treatment technologies (activated sludge, rotary biological discs, percolating beds) must be improved since they are not able to remove emerging contaminants such as enteric pathogens (bacteria and virus). These pollutants are difficult to remove from the wastewater and lead to adverse consequences to human health. Advanced oxidation processes, such as single and catalytic ozonation, appear as suitable complements to conventional processes. Catalytic ozonation was carried out using a low-cost material, a volcanic rock. Single and catalytic ozonation were capable of promoting total Escherichia coli removal from municipal wastewater after 90 min of contact. The presence of volcanic rock increases disinfection efficiency since E. coli regrowth was not observed. The identified viruses (Norovirus genotype I and II and JC virus) were completely removed using catalytic ozonation, whereas single ozonation was not able to eliminate JC virus even after 150 min of treatment. The higher performance of the catalytic process can be explained by the formation of hydroxyl radicals, proving that disinfection occurs in the liquid bulk and not due to adsorption at the volcanic rock.

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“…The research on wastewater contamination by human polyomaviruses dates back to 2000, when the detection of BKPyV and JCPyV was first reported in urban sewage of Barcelona (Bofill-Mas et al 2000 ). Later on, both of these viruses were detected in urban sewage of various geographical locations including Italy (Iaconelli et al 2015 ), Brazil (Comerlato et al 2016 ), Australia (Ahmed et al 2010 ), and Egypt (Ahmed et al 2019 ). In the present study, we reported the detection of BKPyV and JCPyV in sewage water of Lahore for the first time, indicating the ubiquitous presence of BKPyV and JCPyV in the Pakistani population.…”

Section: Discussionmentioning

confidence: 99%

Detection of BK and JC polyomaviruses in sewage water of the urban areas of Lahore, Pakistan

Ijaz,

Shahzad,

Farhan Ul Haque

2023

Biologia

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The surveillance of sewage water has become an extremely essential tool to trace the circulation of viruses in a population and to predict the outbreak of viral diseases. Sewage monitoring is important for those viruses which cause subclinical infections since it is difficult to determine their prevalence. Polyomaviruses are ubiquitously present, circular double-stranded DNA viruses that can infect humans as well. Among all human polyomaviruses, BK polyomavirus and JC polyomavirus associated with the development of aggressive diseases in immunocompromised individuals, are highly prevalent. This study aimed to investigate the presence and the quantitative prevalence of these two disease-associated human polyomaviruses in sewage water collected from six drains of Lahore, Pakistan. The viruses present in the environmental samples were concentrated by PEG method before isolating viral nucleic acids. Conventional PCR amplifications were performed for molecular detection of BK polyomavirus and JC polyomavirus targeting their large tumor antigen genetic region. The presence of BK polyomavirus and JC polyomavirus was confirmed in the DNA extracted from concentrated sewage samples of each drain by performing both qualitative and quantitative PCR. Our data shows that the viral load ranged from 1278 to 178368 copies per µg of environmental DNA for BK polyomavirus and 5173 to 79129 copies per µg of environmental DNA for JC polyomavirus. In conclusion, here we report first time the detection of BK polyomavirus and JC polyomavirus in sewage water collected from six main drains in urban areas of Lahore, Pakistan showing the high prevalence of these viruses in the Pakistani population. This assay could be used as a proxy to determine the prevalence of these viruses in the Pakistani population.

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Distribution and genetic diversity of the human polyomaviruses JC and BK in surface water and sewage treatment plant during 2009 in Porto Alegre, Southern Brazil

Cited by 4 publications

References 41 publications

Occurrence of Polyomaviruses in Recreational Freshwaters from Southern Brazil

Occurrence of Polyomaviruses in Recreational Freshwaters from Southern Brazil

Removal of Enteric Pathogens from Real Wastewater Using Single and Catalytic Ozonation

Detection of BK and JC polyomaviruses in sewage water of the urban areas of Lahore, Pakistan

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