Enzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmission

Abstract

Objective:to evaluate the potential contamination of enzymatic detergent from its reuse and to identify the microbiological profile in the solution used to clean gastrointestinal endoscopic devices.Method:cross-sectional study based on microbiological analysis of 76 aliquots of 19 different enzymatic detergent solutions used to clean endoscopic devices. The aliquots were homogenized, subjected to Millipore® 0.45 µm membrane filtration and the presumptive identification of microorganisms was performed by biochemical-physiological methods according to previously established specific bacterial groups that are of clinical and epidemiological relevance.Results:the mean values, as well as the standard deviation and the median, of the enzymatic detergent microbial load increased as the solution was reused. There was a significant difference between the means of after first use and after fifth reuse. A total of 97 microorganisms were identified, with predominance of the coagulase-negative Staphylococcus, Pseudomonas spp., Klebsiella spp., Enterobacter spp. genus, and Escherichia coli species.Conclusion:the reuse of the enzymatic detergent solution is a risk to the safe processing of endoscopic devices, evidenced by its contamination with pathogenic potential microorganisms, since the enzymatic detergent has no bactericidal property and can contribute as an important source for outbreaks in patients under such procedures.

Como realizamos o processamento de endoscópios flexíveis - Relato de experiência

“…O processamento dos endoscópios desperta grande preocupação, pois a superfície interna e externa são expostas a um elevado número de microorganismos a partir do seu contato com o aparelho digestivo, o que enfatiza a necessidade de uma desinfecção adequada. Evidências sugerem que a transmissão de patógenos através dos aparelhos endoscópicos é um evento muito raro quando as diretrizes de reprocessamento são seguidas de forma correta (Mati et al,2019).…”

Avaliação de dois métodos de limpeza manual no processamento de endoscópio gastrointestinal

“…(8) Recente estudo em nosso país com objetivo de avaliar a carga microbiana presente na solução de detergente enzimático durante a limpeza manual de aparelhos endoscópicos gastrointestinais, evidenciou que a prática do reuso da solução de limpeza favorece a contaminação dos endoscópios com microrganismos potencialmente patogênicos, comprovando a importância do uso da solução conforme recomendação do fabricante bem como órgãos e guias de recomendações. (21).…”