Control of airborne microbes in a poultry setting using Dioxy MP 14

Mbamalu, Oluchi; Uebel, R.; Meki, B

doi:10.1590/1516-635x170177-86

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…A total of 99 samples were collected aseptically, comprising seven different types of samples, including air from the insides of poultry shades ( n = 31), feces from sick birds ( n = 32), and the intestinal organs (trachea, intestine, liver, lung, and egg yolk material; n = 36) of dead birds. Air sampling was done using the settle plate method as previously described by other [ 33 ] with some modifications. In brief, instead of nutrient agar, here, eosin methylene blue (EMB) agar plates were exposed at 1 m above the ground to different corners of the poultry shades for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Molecular Detection of Avian Pathogenic Escherichia coli (APEC) for the First Time in Layer Farms in Bangladesh and Their Antibiotic Resistance Patterns

et al. 2020

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Avian pathogenic Escherichia coli (APEC) causes significant economic losses in poultry industries. Here, we determined for the first time in Bangladesh, the prevalence of APEC-associated virulence genes in E. coli isolated from layer farms and their antibiotic resistance patterns. A total of 99 samples comprising internal organs, feces, and air were collected from 32 layer farms. Isolation was performed by culturing samples on eosin–methylene blue agar plates, while the molecular detection of APEC was performed by PCR, and antibiograms were performed by disk diffusion. Among the samples, 36 were positive for the APEC-associated virulence genes fimC, iucD, and papC. Out of 36 isolates, 7, 18, and 11 were positive, respectively, for three virulence genes (papC, fimC, and iucD), two virulence genes, and a single virulence gene. Although the detection of virulence genes was significantly higher in the internal organs, the air and feces were also positive. The antibiograms revealed that all the isolates (100%) were resistant to ampicillin and tetracycline; 97.2%, to chloramphenicol and erythromycin; 55.5%, to enrofloxacin; 50.0%, to norfloxacin and ciprofloxacin; 19.4%, to streptomycin; 11.1%, to colistin; and 8.33%, to gentamicin. Interestingly, all the isolates were multidrug-resistant (MDR). Spearman’s rank correlation coefficient analysis revealed the strongest significant correlation between norfloxacin and ciprofloxacin resistance. This is the first study in Bangladesh describing the molecular detection of APEC in layer farms. Isolated APEC can now be used for detailed genetic characterization and assessing the impact on public health.

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Section: Methodsmentioning

confidence: 99%

Molecular Detection of Avian Pathogenic Escherichia coli (APEC) for the First Time in Layer Farms in Bangladesh and Their Antibiotic Resistance Patterns

et al. 2020

View full text Add to dashboard Cite

show abstract

“…A total of 99 samples including air from the insides of poultry shades (n = 31), feces from sick birds (n = 32), and the internal organs (trachea, intestine, liver, lung, and egg yolk material; n = 36) of dead birds were collected aseptically from 32-layer farms located in Mymensingh district, Bangladesh during January-November 2019. The settle plate method was used for air sampling following previously described by protocols [20] with some modifications. In brief, instead of nutrient agar, here, eosin methylene blue (EMB) agar plates were exposed at 1 m above the ground to different corners of the poultry shades for 10 min.…”

Section: Sample Collection and Processingmentioning

confidence: 99%

Genomic characteristics, virulence and antimicrobial resistance in avian pathogenic Escherichia coli MTR_BAU02 strain isolated from layer farms in Bangladesh

Ievy

Hoque

Islam

et al. 2022

Preprint

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Background: Colibacillosis, caused by avian pathogenic Escherichia coli (APEC), is one of the most significant infectious diseases affecting poultry worldwide. APEC is one of the leading causes of mortality and morbidity associated with significant economic losses in the poultry industry. Objective: This study was aimed to determine the genomic diversity, virulence factor genes (VFGs) and antimicrobial resistance (AMR) genes in the APEC isolated from layer chickens using whole-genome sequencing (WGS). Method: APEC MTR_BAU02 strain was isolated from the sick and dead birds. Genomic DNA from APEC MTR_BAU02 strain was extracted using commercial DNA extraction kit, WGS libraries were prepared using the Nextera DNA Flex Library Prep Kit, and finally, paired-end (2*250) WGS performed using Illumina MiSeq sequencer. Results: The genome size of strain APEC MTR_BAU02 is 4,924,680 bp with a GC content of 51.1% and 4,681 protein-coding sequences. Among the annotated WGS reads, 99.71% reads mapped to Enterobacteriaceae genomes. Based on the phylogenetic analysis of the APEC MTR_BAU02 genome and 99 reference genomes of E. coli, the APEC MTR_BAU02 genome showed sequence similarities with enterotoxigenic E. coli strains isolated from infections of different clinical severity. Metabolic functional annotations detected 380 SEED subsystems including genes coding for carbohydrate metabolism (17.34%), amino acid and derivatives (14.20%), protein metabolism (10.64%), cofactors, vitamins, prosthetic groups and pigments (7.49%), respiration (4.72%), membrane transport (4.49%), stress response (4.47%), motility and chemotaxis (4.46%), and virulence, disease and defense (2.22%). We also detected 92 VFGs and 122 AMR genes in the APEC MTR_BAU02 strain. Conclusion: Assessment of these genomic features with functional genomic validation in commonly occurring serogroups of APEC will increase our understanding of the molecular pathogenesis, pave the way to early diagnosis and more effective control of colibacillosis, and improved animal welfare.

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Control of airborne microbes in a poultry setting using Dioxy MP 14

Cited by 2 publications

References 23 publications

Molecular Detection of Avian Pathogenic Escherichia coli (APEC) for the First Time in Layer Farms in Bangladesh and Their Antibiotic Resistance Patterns

Molecular Detection of Avian Pathogenic Escherichia coli (APEC) for the First Time in Layer Farms in Bangladesh and Their Antibiotic Resistance Patterns

Genomic characteristics, virulence and antimicrobial resistance in avian pathogenic Escherichia coli MTR_BAU02 strain isolated from layer farms in Bangladesh

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