Seroprevalence of Salmonella and Mycoplasma in commercial broilers, backyard chickens, and spent hens in the region of Triângulo Mineiro, State of Minas Gerais, Brazil

Silva, Cbc; Chagas, WF; Santos, R C dos; Gomes, L.P.; Ganda,; Lima, Anna Monteiro Correia

doi:10.1590/1516-635x170157-62

Cited by 9 publications

(9 citation statements)

References 6 publications

(4 reference statements)

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“…These results fairly close to that obtained by Amer and El-Ghany [43] who reported that the prevalence of MG and MS was 44.35% and 63%, respectively, in breeder chicken flocks by SPA test but differ than that obtained by Silva et al . [44] who reported negative results for Salmonella pulloru m, Salmonella gallinarum , and MG and only positive results (2/135, 1.48%) for MS using SPA test.…”

Section: Resultsmentioning

confidence: 99%

Molecular identification of Mycoplasma synoviae from breeder chicken flock showing arthritis in Egypt

2019

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Aim: Arthritis is one of the most economic problems facing poultry industry worldwide. The study was done to detect possible causes of arthritis in breeder chicken flock with emphasis on molecular identification of Mycoplasma synoviae (MS). Materials and Methods: This study was carried on chicken from broiler breeder flock of 57 weeks' age in Dakahlia, Egypt, suffered from arthritis with frequently 5-7% decrease in egg production, reduced fertility, and hatchability. Forty blood samples were randomly collected from individual birds in sterile tubes and used for serum separation. Serum samples were tested using serum plate agglutination (SPA) test against colored antigens for Mycoplasma gallisepticum (MG), MS, and Salmonella gallinarum-pullorum (SGP). On the other hand, 24 joint samples were collected. Of those 24 samples, 12 joint samples were subjected to bacteriological examination, while the other 12 were utilized for molecular diagnosis by polymerase chain reaction (PCR) for MS and avian reovirus (ARV). Results: SPA test results revealed the presence of antibodies against MG, MS, and SGP in tested sera in rates of 14/40 (35%), 35/40 (87.5%), and 9/40 (22.5%), respectively. Furthermore, 19 bacterial isolates were recognized from joint samples and identified as five Staphylococcus spp., nine Escherichia coli, three SGP, one Citrobacter, and one Proteus. The identified Staphylococcal isolates were three coagulase-positive staphylococci (two Staphylococcus aureus and one Staphylococcus hyicus) and two coagulase-negative staphylococci (one Staphylococcus epidermidis and one Staphylococcus lentus), while E. coli isolate serotypes were 1 O11, 2 O55, 3 O78, 1 O124, 1 O125, and 1 untyped. PCR proved that 12/12 (100%) samples were positive for MS variable lipoprotein hemagglutinin A (vlhA) gene, while ARV was not diagnosed in any of the examined samples. Four amplified vlhA gene of MS isolates (named MS-2018D1, MS-2018D2, MS-2018D3, and MS-2018D4) was successfully sequenced. Analysis of phylogenetic tree revealed the presence of 100% identity between each two sequenced isolates (isolates MS-2018D1 and MS-2018D4 and also isolates 2018D2 and MS-2018D3). However, the nucleotide similarity between four isolates was 88.6%. On the other hand, our field isolates MS-2018D1, MS-2018D4, MS-2018D2, and MS-2018D3 showed nucleotide identity with vaccine strain MS-H 98.4%, 98.4%, 88.1%, and 88.1%, respectively. Furthermore, the nucleotide similarities with field strains from Argentina ranged between 87.8% and 98.6%. Conclusion: Four field isolates of MS were identified in examined broiler breeder flock. A phylogenetic study of these isolates revealed the variation between isolated MS strains and vaccine strain. Therefore, further studies are required for evaluating the vaccine efficacy against the present field isolates of MS. In addition, application of MS immunization of breeder flocks is necessary for proper control of the disease.

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Section: Resultsmentioning

confidence: 99%

Molecular identification of Mycoplasma synoviae from breeder chicken flock showing arthritis in Egypt

2019

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“…Our finding constitutes the first report of MG prevalence in poultry in Mozambique and is approximately in agreement with report from Zimbabwe (<33%) [ 13 ]. Higher prevalence was reported in backyard chickens in Benin (62%) [ 15 ], Botswana (57.8%) [ 14 ], South Africa (63%) [ 16 ], Ecuador (73%) [ 22 ], Bangladesh (58.9%) [ 23 ], Argentina (68.6–100%) [ 24 ], Ethiopia (67.7%) [ 17 ], and Brazil (53.3%) [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

Serological Screening Suggests Extensive Presence ofMycoplasma gallisepticumandMycoplasma synoviaein Backyard Chickens in Southern Mozambique

Júnior

Taunde

Zandamela³

et al. 2017

Journal of Veterinary Medicine

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A total of 459 serum samples from unvaccinated backyard chickens originating from 4 villages in Mandlakazi district, Southern Mozambique, were tested for the presence of Mycoplasma gallisepticum and Mycoplasma synoviae antibodies through commercial enzyme-linked immunoabsorbent assay [ELISA] kits. Anti-MG and anti-MS antibodies were detected in all villages surveyed and the overall seroprevalence was 48.8% [95% CI 39.1–57.8] and 84.5% [95% CI 76.8–90.4], respectively. The risk of being seropositive for both diseases was higher [P < 0.05] in Chidenguele village than other villages. It is concluded that MG and MS serum antibodies are present in backyard chickens.

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“…M. synoviae seroprevalence in layers is high worldwide [10,11,16,23,28,[31][32][33][34][35]. Surveys performed in France reported a seroprevalence of 60 and 68% for MS in laying hen flocks in 1999 and 2006, respectively [18,36].…”

Section: Discussionmentioning

confidence: 99%

Investigation on eggshell apex abnormality (EAA) syndrome in France: isolation of Mycoplasma synoviae is frequently associated with Mycoplasma pullorum

et al. 2020

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Background: Mycoplasma synoviae (MS) is known to cause Eggshell Apex Abnormality (EAA) syndrome characterized by an altered shell surface with increased translucency on the apex. However, no large-scale studies have been conducted to obtain prevalence data of EAA and MS isolates associated to this syndrome. This manuscript reports the results of two field studies performed in the French poultry industry (2015-2017): focusing mainly on investigation of presence and prevalence of EAA in different types of laying hen flocks (phase 1), and isolation of MS strains from EAA-infected flocks (phase 2). Results: The first survey included 77 farms of commercial layers in three French egg-production regions, hosting 40 flocks in alternative systems (ALT) and 56 in furnished cages (FC). Seven flocks (4 FC and 3 ALT) presented EAA clinical signs, giving a prevalence of 7.3% in this studied sample. A second independent field study was conducted to identify MS by in vitro cultivation and PCR in samples from 28 flocks with clinical signs of EAA. Different types of biological specimens were collected in EAA-affected flocks and submitted to the laboratory. M. synoviae was detected in 25/28 flocks, from both production systems (5/5 ALT and 20/23 FC). Detection of MS was significantly higher in tracheal swabs (59%) than in cloacal (10.5%), albumen (3.6%) and egg yolk (1.1%) swabs. It is worth to mention that attempts to clone MS from positive samples were often hampered by the presence of another Mycoplasma species, which showed fast growing behaviour in the selective media used in this study (Frey Medium 4 and Frey Medium 4 supplemented with erythromycin). The use of MALDI-TOF mass spectrometry in combination with next-generation sequencing (NGS) results allowed the identification of this fast growing mycoplasma as Mycoplasma pullorum, which was detected in 14 of the 25 (56%) MS-positive flocks.

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Seroprevalence of Salmonella and Mycoplasma in commercial broilers, backyard chickens, and spent hens in the region of Triângulo Mineiro, State of Minas Gerais, Brazil

Cited by 9 publications

References 6 publications

Molecular identification of Mycoplasma synoviae from breeder chicken flock showing arthritis in Egypt

Molecular identification of Mycoplasma synoviae from breeder chicken flock showing arthritis in Egypt

Serological Screening Suggests Extensive Presence ofMycoplasma gallisepticumandMycoplasma synoviaein Backyard Chickens in Southern Mozambique

Investigation on eggshell apex abnormality (EAA) syndrome in France: isolation of Mycoplasma synoviae is frequently associated with Mycoplasma pullorum

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