Phenotypic and genotypic resistance profile of Salmonella Typhimurium to antimicrobials commonly used in poultry

Biffi, CP; Stefani, Lenita Moura; Miletti, Luiz Cláudio; Matiello, CA; Backes, RG; Jm, Almeida; Neves, GB

doi:10.1590/1516-635x160293-96

Cited by 14 publications

(12 citation statements)

References 11 publications

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“…The aacC gene was detected in two isolates, although only one of them showed resistance to gentamicin. Silent antimicrobial resistance genes were previously reported in Salmonella isolated from chicken in other studies (Biffi et al 2014;El-Sharkawy et al 2017); however, these genes may activate in vivo or spread to other bacteria, thereby posing the same risk as active genes (El-Sharkawy et al 2017). Both the aacC and aadA resistance genes were previously detected in S. Kentucky isolated from an outbreak in Slovenia caused by travel to Egypt (Majt an et al 2006).…”

Section: Discussionmentioning

confidence: 94%

“…All the tested Salmonella isolates harbouring the aadA2 gene showed phenotypic resistance to streptomycin but not gentamicin, which is consistent with a previous report in chicken Salmonella isolates in Egypt (El-Sharkawy et al 2017). However, an association between the aadA gene and gentamicin resistance in chicken Salmonella was reported in Brazil (Biffi et al 2014). The aacC gene was detected in two isolates, although only one of them showed resistance to gentamicin.…”

Section: Discussionmentioning

confidence: 99%

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Antibiogram and genetic diversity of Salmonella enterica with zoonotic potential isolated from morbid native chickens and pigeons in Egypt

et al. 2018

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Antibiogram and genetic diversity of Salmonella enterica with zoonotic potential isolated from morbid native chickens and pigeons in Egypt

et al. 2018

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“…); for quinolone resistance determining regions linked to resistance ( gyrA, gyrB, parC , parE ) (Biffi et al . ); for integrons ( Int1 Cls1 , intB , IntA ) (Weill et al . ; Lynne et al .…”

Section: Methodsmentioning

confidence: 99%

“…) whereas, the resistance in Salmonella against quinolones has been linked with various gene mutations (Biffi et al . ). Efflux pumps, present in bacteria and eukaryotic organisms are also important transport proteins involved in the expulsion of toxic substances including clinically important antimicrobials (Webber and Piddock ).…”

Section: Introductionmentioning

confidence: 97%

“…The resistance in Salmonella serovars against various betalactams due to the production of beta-lactamases enzymes has been reported (Hasman et al 2005). Different genes and/or mutations in particular genes result in emergence of antimicrobial resistance in different bacteria for example, resistance against aminoglycosides is linked with expression of aminoglycoside-modifying enzymes such as acetyltransferases, phosphotransferases and nucleotidyltransferases (Matayoshi et al 2015) whereas, the resistance in Salmonella against quinolones has been linked with various gene mutations (Biffi et al 2014). Efflux pumps, present in bacteria and eukaryotic organisms are also important transport proteins involved in the expulsion of toxic substances including clinically important antimicrobials (Webber and Piddock 2003).…”

Section: Introductionmentioning

confidence: 99%

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Detection and characterization of multidrug‐resistant Salmonella enterica serovar Infantis as an emerging threat in poultry farms of Faisalabad, Pakistan

et al. 2019

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Aims The aim of this study was the molecular identification of Salmonella enterica serovar Infantis (S. Infantis) isolated from poultry samples and their antimicrobial resistance and virulence profiling. Methods and Results A total of 149 isolates, belonging to genus Salmonella, originally isolated from 340 suspected poultry post mortem specimens reported by us earlier were preliminary identified as Salmonella by biochemical methods and confirmed by polymerase chain reaction targeting genus‐specific gene invA. Targeting serovar‐specific gene fragment (fljB) resulted in confirmation of 54 isolates as S. Infantis which were further confirmed by sequencing of 16S RNA and fljB genes. Swimming and swarming motilities were detected in 98·1 and 11·1% isolates respectively. Phenotypic disc diffusion assay against 23 antimicrobial agents showed the highest resistance against pefloxacin (PEF) (94·4%), chloramphenicol (83·3%) and imipenem (77·7%) while 5·3% isolates showed extended‐spectrum β‐lactamase production. Fifty‐nine genes reported for antimicrobial resistance and 12 for conferring virulence were targeted. The most prevalent resistance gene for aminoglycosides was aadA (42·3%), for quinolone resistance determining region parE (62·5%), for penicillin's Int1 (62·9%), for chloramphenicol cat3 (66·1%) and for beta‐lactams blaTEM‐1 (44·4%). Among efflux pump coding genes, armA showed highest (74·2%) prevalence and for virulence, a high prevalence of SopE (89·2%) showed the zoonotic potential of the isolates. The activity of efflux pumps was detected through Ethidium Bromide‐agar method. Conclusions Poultry could act as reservoirs of multidrug resistance Salmonella. Significance and Impact of the Study We firstly report the prevalence and molecular characterization of virulence/drug resistance in S. Infantis from this region and the results may contribute to designing precisely targeted therapy. This study has also highlighted the possible emergence of S. Infantis with zoonotic potential.

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Highly clonal relationship among Salmonella Enteritidis isolates in a commercial chicken production chain, Brazil

et al. 2020

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In this study, we described the comparison among pulsed-field gel electrophoresis (PFGE), random amplified polymorphic DNA (RAPD), ribotyping, and PCR-ribotyping methods for subtyping Salmonella Enteritidis isolated from an industrial chicken production chain. One hundred and eight S. Enteritidis were isolated at all stages of poultry meat processing plant. These isolates were pheno-and genotypically characterized by using antimicrobial susceptibility test, phage typing, RAPD, PFGE, ribotyping, and PCR-ribotyping. The highest antibiotic resistance rates were observed for enrofloxacin (18.5%) followed by furazolidone (15.7%), cefoxitin (1.8%), ciprofloxacin, and ampicillin with 0.9% each one, while seven isolates (6.4%) were pan-susceptible. Most strains belonged to the globally disseminated phage type PT4 (n = 74; 69.2%). Additionally, we identified strains belonging to phage types PT1 (n = 19; 17.8%) and PT7a (n = 14; 13.1%). Moreover, our results showed that these four molecular methods indicate similar results showing high similarity (≥ 90%) among S. Enteritidis strains, suggesting that these isolates appear to be from a common ancestor being spread at all stages of the poultry production chain. In summary, the combined molecular approaches of these methods remain a suitable alternative to efficiently subtyping S. Enteritidis in the absence of high-resolution genotyping methods and these results may serve as a baseline study for development of mitigation strategies.

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Phenotypic and genotypic resistance profile of Salmonella Typhimurium to antimicrobials commonly used in poultry

Cited by 14 publications

References 11 publications

Antibiogram and genetic diversity of Salmonella enterica with zoonotic potential isolated from morbid native chickens and pigeons in Egypt

Antibiogram and genetic diversity of Salmonella enterica with zoonotic potential isolated from morbid native chickens and pigeons in Egypt

Detection and characterization of multidrug‐resistant Salmonella enterica serovar Infantis as an emerging threat in poultry farms of Faisalabad, Pakistan

Highly clonal relationship among Salmonella Enteritidis isolates in a commercial chicken production chain, Brazil

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