In-Vitro Antiviral Activities of Extracts of Plants of The Brazilian Cerrado against the Avian Metapneumovirus (aMPV)

Kohn, LK; Foglio, MA; Rodrigues, Rodney Alexandre Ferreira; Sousa, IM de Oliveira; Martini, M Y; Padilla, MA; Neto, DF de Lima; Arns, Clarice Weis

doi:10.1590/1516-635x1703275-280

Cited by 32 publications

(22 citation statements)

References 17 publications

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“…Ramalingam et al [28] reported that the ethanolic extracts of Andrographis paniculata have the highest antiviral inhibitory effects against dengue virus in Vero cells. The screening of plants as possible sources of antiviral agents has led to the discovery of potent inhibitors of in vitro viral replication, thereby increasing the probability of identifying new bioactive plant compounds [29]. These findings suggest the appropriate species and concentration of plant extract that could effectively inhibit PRRSV replication, with both T. triandra and C. sappan extracts being highly effective in inhibiting PRRSV infection in vitro by interfering with viral attachment and inhibiting viral replication and/or virus release, respectively.…”

Section: Discussionmentioning

confidence: 99%

In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus

et al. 2020

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Background: Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) results in economic losses in the swine industry globally. Several studies have investigated the use of plant extracts in the prevention and control of PRRS outbreaks. Thai medicinal plants may be useful for treating PRRSV infection in pigs. Therefore, we investigated the in vitro anti-PRRSV and antioxidant properties of seven Thai medicinal plants: Caesalpinia sappan Linn., Garcinia mangostana Linn., Houttuynia cordata, Perilla frutescens, Clinacanthus nutans, Phyllanthus emblica, and Tiliacora triandra. Results: Using antiviral screening, we observed that T. triandra extract strongly inhibited PRRSV infectivity in MARC-145 cells [virus titer 3.5 median tissue culture infective dose (TCID 50)/ml (log10)] at 24 h post-infection, whereas C. sappan extract strongly inhibited PRRSV replication [virus titer 2.5 TCID 50 /ml (log10)] at 72 h post-infection. C. sappan extract had the highest total phenolic content [220.52 mM gallic acid equivalent/g] and lowest half-maximal inhibitory concentration [1.17 mg/ml in 2,2-diphenyl-1-picrylhydrazyl and 2.58 mg/ml in 2,2-azino-bis (3ethylbenzothiazo-line-6-sulfonic acid) diammonium salt]. Conclusion: T. triandra extract could inhibit PRRSV infectivity, whereas C. sappan extract was the most effective in inhibiting PRRSV replication in MARC-145 cells. This study elucidates the antiviral activities of Thai medicinal plant extracts in vivo. The results promise that Thai medicinal plant extracts, particularly T. triandra and C. sappan extracts, can be developed into pharmaceutical drugs for the prevention of PRRS in pigs.

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Section: Discussionmentioning

confidence: 99%

In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus

et al. 2020

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“…Post adsorption studies involved first infecting the cells with the HSV-1 virus followed an hour later by the addition of crude extracts of D. integrifolia. 20 From our results, we found that the methanol flower extract of D. integrifolia inhibited HSV-1 infection more strongly postadsorption than during other treatment durations. This may suggest that this extract may not prevent the entry of HSV-1 into the Vero cell, but it acts after the virus has already penetrated the cell.…”

Section: Discussionmentioning

confidence: 55%

“…To evaluate the mechanism of antiviral activity vis-à-vis the step of the viral cycle where replication of HSV-1 was inhibited, different treatment protocols were followed. 20,21 Pre-treatment studies involved treating the HSV-1 virus with each of the crude extracts of D. integrifolia before infecting the cells. 20 This was done to evaluate the virus inactivation capacity of these extracts.…”

Section: Discussionmentioning

confidence: 99%

“…20,21 Pre-treatment studies involved treating the HSV-1 virus with each of the crude extracts of D. integrifolia before infecting the cells. 20 This was done to evaluate the virus inactivation capacity of these extracts. Further, adsorption studies involved adding each of the crude extracts of D. integrifolia to the cells before HSV-1 infection.…”

Section: Discussionmentioning

confidence: 99%

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Antiviral Activity of Dicrocephala Integrifolia (Kuntze) Against Herpes Simplex Type-1 Virus: An in vitro Study

Hadun¹,

Mbaria²,

Aboge³

et al. 2018

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Introduction: At present, acyclovir is commercially available as the drug of choice for managing herpes simplex type I (HSV-1) viral infection. However, the high prevalence of the infection coupled with the emergence of resistant viral strains has limited its effectiveness. Thus, the development of novel antiviral agents is crucial. Practitioners of herbal medicine in Kenya make use of Dicrocephala integrifolia (DI) for the management of several diseases including viral infections. However, information on the efficacy of this plant against HSV-1 viral infection is not available. The aim of the present study was to determine the in vitro antiviral activity of crude extracts of DI against HSV-1. Methods: Leaves, roots, flowers and stems of DI were extracted using water (W) and methanol (ME) and qualitatively screened to identify the phytoconstituents present. Furthermore, the anti HSV-1 activity of the obtained extracts was evaluated on Vero cell lines using the 3-[4, 5 dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide] assay. The 50% cytotoxic concentration (CC 50 ) and 50% effective concentration (EC 50 ) of each extract was determined using regression analysis. The effects of crude DI extracts on adsorption and post-adsorption stages of the HSV-1 replication cycle was evaluated against acyclovir using a cytopathogenic inhibition assay. Results: Alkaloids, glycosides, flavonoids, phenols, saponins, tannins and terpenoids were found to be present in the extracts. The CC 50 values of the aqueous DI extracts was in the range 71.31 ± 2.65 to >100 µg/ml compared to >100 µg/ml of acyclovir. The EC 50 values of crude extracts of DI on the pre-adsorptive phase of HSV-1 activity was in the range 54.45±3.45 to >100µg/ml compared to 4.772±7.81µg/ml of acyclovir whilst the EC 50 value of the crude extracts of DI on the post-adsorptive phase of HSV-1 activity was in the range 45.270±4.31 to >100µg/ml compared to >100µg/ml of acyclovir. Conclusion: The results suggest that crude extracts of DI may be a reservoir of phytochemicals with potentially good efficacy against HSV-1.

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“…Ten-fold serial dilutions of HSV-1 and virus-treated extracts of HSV-1 were prepared prior to the infection. The number of plaque-forming units was determined and a decrease of virus titer was calculated [19]:…”

Section: Infectious Virus Yield Reduction Assaymentioning

confidence: 99%

Effect of fluorinated N-alkylthioamides on HSV-1 multiplicity

et al. 2018

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The number of promising viral targets and classes of compounds with substantial antiherpetic properties considerably increased during the last decade. However, no new effective and lowtoxicity clinical drugs against both wild-type viruses and drug-resistant strains have appeared. This situation makes the search for new antiherpetic drugs and their new targets a high priority task. Aim. To study the effect of fluorinated N-alkylthioamides on the herpes simplex virus-1 (HSV-1). Methods. The influence of these compounds on the multiplicity and infectivity of HSV-1 was determined by the MTT-assay, virucidal assay, adsorption and penetration assays, PCR and infectious virus yield reduction assay. Results. The 10S-23 and 10S-24 compounds prevented the adsorption and penetration of HSV-1 into cells by up to 26%. HSV-1 DNA replication was moderately inhibited by the 10S-24 compound (up to 39 %). The 10S-23 and 10S-24 compounds in concentrations of 100-33 μg/ml reduced HSV-1 virus production by 70-99% and >99%, respectively. Conclusion. The 10S-24 compound may be used as a therapeutic agent to reduce the penetration, replication and translation of HSV-1.

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In-Vitro Antiviral Activities of Extracts of Plants of The Brazilian Cerrado against the Avian Metapneumovirus (aMPV)

Cited by 32 publications

References 17 publications

In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus

In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus

Antiviral Activity of Dicrocephala Integrifolia (Kuntze) Against Herpes Simplex Type-1 Virus: An in vitro Study

Effect of fluorinated N-alkylthioamides on HSV-1 multiplicity

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