Performance and reference intervals of thrombin generation test: results from the Brazilian longitudinal study of adult health (ELSA-Brasil). A cross-sectional study

Rios, Danyelle Romana Alves; Barreto, Sandhi Maria; Ferreira, Letícia Gonçalves Resende; Batista, Thaís Resende; Silva, Ana Paula Ferreira; Júnior, Wander Valadares de Oliveira; Maluf, Chams Bicalho; Ferriani, Maria das Graçãs Carvalho; Figueiredo, Rachel

doi:10.1590/1516-3180.2021.0425.r1.07102021

Cited by 3 publications

(3 citation statements)

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“…In men, Peak median was significantly lower compared to that in women ( p < 0.05). Also in the ELSA Brasil study, an increase in Peak was observed in women (mean [SD]: 338.6 [47.4] vs. 384.8 [54.4] nM, respectively), although almost all indicators in this study were sex associated 6 . Another study also found Peak increase in women (mean [SD]: males: 236 [52.2] vs. females: 259 [53.3]) 8 .…”

Section: Discussioncontrasting

confidence: 49%

“…The RIs for TGT parameters in PPP obtained in this study were comparable with some works dedicated to developing RIs, which used a trigger reagent with a concentration of 5 pM. In a recent large ELSA Brasil study ( n = 620 healthy adults aged 35–74), the following RI values for PPP were obtained: Lagtime (min): 1.4–3.7; ETP (nM/min): 1413.6–2658.0; Peak (nM): 256.4–479.0; ttPeak (min): 3.4–6.7; Normalized ETP: 0.7–1.4 6 . Although TGT in our study was performed under different preanalytical conditions, our data are consistent with the results of this work.…”

Section: Discussionmentioning

confidence: 99%

“…An ISTH SSC survey revealed disparities in various stages of thrombin generation (TG) setup among laboratories, including blood collection needle type, blood tubes, centrifugation, sample handling and thawing, reagents, sample dilution, calibration, and data normalization 5 . Few studies have established reference intervals (RIs) for TGT's clinical implementation, 6–9 mainly focusing on RIs determination in platelet‐poor plasma (PPP) under 1 and 5 pmol/L TF (Tissue factor) activation. Furthermore, these studies employ diverse data presentation methods.…”

Section: Introductionmentioning

confidence: 99%

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Reference intervals and biological variation in parameters of the thrombin generation test in healthy individuals

Olga,

Yuliya,

Vitaly

et al. 2023

Int J Lab Hematology

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IntroductionEstablish the referenceintervals (RIs) and analyze biological variability (BV) to introduce the thrombin generation test (TGT) into clinical practice.MethodsTo determine the RIs parameters of TGT, we analyzed platelet‐poor plasma (PPP) (n = 123), rich (PRP) (n = 76), and microparticle‐mediated TGT (MP‐TGT) (n = 32) in healthy participants. For the BV study, we collected samples from five participants over 5 weeks. A nested analysis of variance (ANOVA) was performed to evaluate the BV results.ResultsThe between‐individual variation (CVG), within‐individual variation (CVI), analytical variation (CVA) for TGT on PPP for all parameters were from 5.5% to 17.3%, 5.4% to 17.7%, and 2.6% to 5.3%, respectively. For PRP, the CVG, CVI, and CVA were ranged from 3.0% to 23.7%, 8.4% to 23.0%, and 4.1% to 6.9%, respectively. The index of individuality (II) ranged from 0.3 to 3.1 for PPP and from 0.3 to 4.5 for PRP. The reference change value (RCV) for PPP was from 19.8% to 50.1%, while for PRP, it was 27.2% to 66.5%. We recommend using the RIs for the parameters ETP (nM/min): 1101.6–2332.1 and Peak (nM): 163.5–381.3 for PPP and ETP (nM/min): 1088.5–2634.9; Peak (nM): 72.6–210.7 for PRP. The resulting MP‐TGT are highly dependent on age require a larger sample.ConclusionFor TGT on PPP and PRP the RIs developed on our population for Peak and ETP parameters can be used. Time parameters: Lagtime and ttPeak, min with II < 0.6, require monitoring over time with RCV calculation.

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Section: Discussioncontrasting

confidence: 49%

Section: Discussionmentioning

confidence: 99%