Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and chronic renal disease: a cross-sectional study

Rojo-Báez, Indira; Castro, Daniel Omar Rivera; Gutiérrez, Sarahí Salas; López, Edgar Dehesa; Aguilar‐Lemarroy, Adriana; Jave-Suárez, Luis F; Ureta, Hipólito Castillo; Montoya, Edith Hilario Torres; Olimón-Andalón, Vicente

doi:10.1590/1516-3180.2019.0304.r2.09122019

Cited by 7 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies suggest that metabolic inflammation may contribute to elevated serum SFRP5 levels, as several diseases with concomitant systemic inflammatory responses have significantly higher SFRP5 levels than the normal baseline [27,[51][52][53]. Similarly, excessive systemic inflammation is also an important feature of PE, which causes extensive damage to the vascular endothelium, leading to the appearance of clinical symptoms [1].…”

Section: Discussionmentioning

confidence: 99%

Elevated Serum SFRP5 Levels During Preeclampsia and Its Potential Association with Trophoblast Dysfunction via Wnt/β-Catenin Suppression

Zhang

Ran

et al. 2021

Reprod. Sci.

View full text Add to dashboard Cite

Preeclampsia (PE) is a life-threatening pregnancy complication associated with diminished trophoblast migration and invasion. Wnt signalling is one of the most important regulators of placentation. Secreted frizzled-related protein 5 (SFRP5) is an antiinflammatory adipokine that may inhibit Wnt signalling. In this study, we aimed to investigate the relationship between SFRP5 and PE and its effect on trophoblast function, as well as the underlying signalling pathways. SFRP5 levels in the serum and placental tissues were detected using enzyme-linked immunosorbent assay and immunohistochemistry, respectively. To evaluate the effect of SFRP5 on Wnt signalling, the human trophoblast cell line HTR8/SVneo was treated with recombinant human SFRP5 and Dickkopf-related protein 1 (Dkk-1, canonical Wnt inhibitor) proteins and lithium chloride (LiCl, canonical Wnt agonist). The migration and invasion ability of HTR8/SVneo cells was evaluated using wound-healing and Matrigel Transwell assays. The activities of multiple matrix metalloproteinases (MMP)-2/9 were detected using gelatin zymography. Expression of glycogen synthase kinase-3 beta (GSK3β) and β-catenin proteins was investigated using western blotting. The serum SFRP5 levels were elevated in patients with PE, but SFRP5 expression was not detected in the placental tissues. Furthermore, SFRP5 inhibited the migration and invasion of HTR8/SVneo cells in vitro, increased GSK3β, and decreased β-catenin expression and MMP-2/9 activity in HTR8/SVneo cells. In conclusion, this study suggests that SFRP5 inhibits trophoblast migration and invasion potentially via the inhibition of Wnt/β-catenin signalling, which might be involved in the development of PE. However, the primary cause of the increased SFRP5 levels needs to be investigated.

show abstract

Section: Discussionmentioning

confidence: 99%

Elevated Serum SFRP5 Levels During Preeclampsia and Its Potential Association with Trophoblast Dysfunction via Wnt/β-Catenin Suppression

Zhang

Ran

et al. 2021

Reprod. Sci.

View full text Add to dashboard Cite

show abstract

“… 91 Likewise, Wnt5a is highly expressed in ischaemic muscles and ECs from mice overexpressing glutaredoxin‐1, an oxidation‐promoting enzyme which is increased in T2DM patients, and exogenous Wnt5a treatment could inhibit the revascularization in hind limb ischaemia via activating the non‐canonical Wnt pathway, 92 indicating a deleterious role of Wnt5a in diabetic foot. Although the increase in serum concentration of Wnt5a and a negative correlation between serum Wnt5a and glomerular filtration rate are detected in patients with DP, 93 more studies tend to prove the protective role of Wnt5a in the development of DP. It has been shown that a weak Wnt5a expression is detected in glomerular mesangium of diabetic rat and glomerular cells cultured in high glucose condition.…”

Section: Roles Of Wnts In T2dm and Related Complicationsmentioning

confidence: 97%

The complex role of Wnt ligands in type 2 diabetes mellitus and related complications

Nie

Wei

et al. 2021

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…However, the effects of non-classical WNT signaling were rarely reported. WNT5A, a typical glycoprotein of the noncanonical pathway, has been verified to have pro-inflammatory functions in urosepsis, nephrogenic diabetes insipidus (NDI), and chronic kidney disease (CKD) (28)(29)(30). WNT5A signaling play potential roles in human placental and malfunction of it can cause preeclampsia (31,32).…”

Section: Introductionmentioning

confidence: 99%

Low-Dose Aspirin Prevents Kidney Damage in LPS-Induced Preeclampsia by Inhibiting the WNT5A and NF-κB Signaling Pathways

Wei

Suo

et al. 2021

Front. Endocrinol.

View full text Add to dashboard Cite

Preeclampsia (PE) is a serious pregnancy-related disease, and patients usually present with a high inflammatory response. Previous studies have suggested that aspirin (ASP) may have a role in alleviating the pathogenesis of preeclampsia. However, whether ASP can improve kidney damage and the mechanism for improving it is currently unclear. Here we optimized a lipopolysaccharide (LPS)-induced PE mouse model to identify the role of ASP in renal protection. We found that ASP treatment ameliorated LPS-induced renal failure and pathological changes, the tubular injury was significantly attenuated by ASP. Administration of ASP decreased the renal expression of pro-inflammatory factors, resulting in reduced kidney inflammation. The number of GALECTIN-3-positive cells was reduced, and the up-regulation of IL-6 and TNF-α was decreased. In addition, ASP also suppressed renal cell apoptosis and oxidative stress. An in vitro study indicated that ASP relieved LPS-induced HK-2 cell damage by inhibiting WNT5A/NF-κB signaling. Collectively, our data suggest that ASP is a useful therapeutic option for PE-related kidney injury.

show abstract

Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and chronic renal disease: a cross-sectional study

Cited by 7 publications

References 20 publications

Elevated Serum SFRP5 Levels During Preeclampsia and Its Potential Association with Trophoblast Dysfunction via Wnt/β-Catenin Suppression

Elevated Serum SFRP5 Levels During Preeclampsia and Its Potential Association with Trophoblast Dysfunction via Wnt/β-Catenin Suppression

The complex role of Wnt ligands in type 2 diabetes mellitus and related complications

Low-Dose Aspirin Prevents Kidney Damage in LPS-Induced Preeclampsia by Inhibiting the WNT5A and NF-κB Signaling Pathways

Contact Info

Product

Resources

About