This study examined blood lactate concentration and removal rate after aerobic and anaerobic maximal exercise test in sedentary middle-age women. METHODS: Ten sedentary middle-age women (aged 52.7±3.3 years) were performed after aerobic (Aero group) and anaerobic maximal exercise (Anaero group) test, VO2max test and Wingate test, by cross-over repeated design with biweekly. The blood was collected from cardinal veins at before and immediately after test for measurement of lactate and lactate dehydrogenase (LDH). And it was also collected from fingertip at before, immediately after and then recovery 5, 10, 15, 30 minutes after each maximal test. Lactate removal rate (%) were calculated by bellow formulas: ={1-[(Lactate at 30 minutes. after test-Resting lactate)/Increased lactate]}×100. RESULTS: The blood lactate concentration from fingertip after both maximal test significantly increased immediately after test (p<.001 respectively), and maintained until recovery of 30 minutes. after test (p<.01, p<.001 respectively) in spite of there were no significant differences between both Aero and Anaero group. There were also no significant differences between both test groups in blood maximum lactate (p=.090) and increased lactate concentration (p=.058). No significant differences were also observed between the two groups in lactate removal rate (p >.05). There are positive correlationship among muscle mass and blood maximum lactate and increased lactate concentration (p<.01). CONCLUSIONS: These results indicate that exercise was performed by anaerobic at end of test in VO2max test. Also suggested that the greater muscle mass may induce the more lactate accumulation, thereby increasing lactate tolerance.