A semi-automated microscopic image analysis method for scoring Ki-67 nuclear immunostaining

Fernezlian, S.M.; Baldavira, C.M.; Souza, M.L.F. de; Farhat, C.; Vilhena, A.F. de; Pereira, J.C.N.; Campos, J.R.M. de; Takagaki, T.; Balancin, M.L.; Ab'Saber, A.M.; Capelozzi, V.L.

doi:10.1590/1414-431x2023e12922

Cited by 1 publication

(1 citation statement)

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“…Immunohistochemical staining of the cellular proliferation marker Ki-67 was used to evaluate the growth of primordial follicles in vitro. Ki-67 is a non-histone DNA-binding protein that is upregulated during cell mitosis [28]. The primordial follicles were considered growing when at least one granulosa cell was Ki-67 positive.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Enhancement of Vascularization and Ovarian Follicle Survival Using Stem Cells in Cryopreserved Ovarian Tissue Transplantation—A Systematic Review

Zaninović,

Bašković,

Ježek

et al. 2024

Biology

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The increase in cancer survival rates has put a focus on ensuring fertility preservation procedures for cancer patients. Ovarian tissue cryopreservation presents the only option for prepubertal girls and patients who require immediate start of treatment and, therefore, cannot undergo controlled ovarian stimulation. We aimed to provide an assessment of stem cells’ impact on cryopreserved ovarian tissue grafts in regard to the expression of growth factors, angiogenesis promotion, tissue oxygenation, ovarian follicle survival and restoration of endocrine function. For this systematic review, we searched the Scopus and PubMed databases and included reports of trials using murine and/or human cryopreserved ovarian tissue for transplantation or in vitro culture in combination with mesenchymal stem cell administration to the grafting site. Of the 1201 articles identified, 10 met the criteria. The application of stem cells to the grafting site has been proven to support vascular promotion and thereby shorten the period of tissue hypoxia, which is reflected in the increased number of remaining viable follicles and faster recovery of ovarian endocrine function. Further research is needed before implementing the use of stem cells in OT cryopreservation and transplantation procedures in clinical practice. Complex ethical dilemmas make this process more difficult.

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Section: Immunohistochemistrymentioning

confidence: 99%