Ophiopogonin D of Ophiopogon japonicus ameliorates renal function by suppressing oxidative stress and inflammatory response in streptozotocin-induced diabetic nephropathy rats

Qiao, Yi; Hu, Jiao; Wang, Feng; Niu, Huimin

doi:10.1590/1414-431x20209628

Cited by 19 publications

(15 citation statements)

References 33 publications

(38 reference statements)

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“…[34,35] These inflammatory cytokines further start the production of a chemotactic factor in the renal tissues, which induced the infiltration of the inflammatory cells. [36] Finally, this process is involved in the expansion of renal injury. TNF-α boosts the chemotactic factors and inflammatory factors, they are involved in the expansion of inflammatory reactions.…”

Section: Discussionmentioning

confidence: 99%

Lycoperoside H protects against diabetic nephropathy via alteration of gut microbiota and inflammation

Zhang

Liu

et al. 2022

J Biochem & Molecular Tox

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It is well known that hyperglycemia leads to the progression and expansion of various micro and macrovascular disease such as diabetic nephropathy (DN).Lycoperoside H (LH) alkaloidal saponin exhibited the antidiabetic effect, but its DN effect is unclear. In this experimental study, we scrutinized the renal protective effect of LH against the streptozotocin (STZ)-induced DN in rats and explore the underlying mechanism. Sprague-Dawley rats were used in this experimental study and an intraperitoneal injection of STZ (45 mg/kg) was used for the induction of diabetes, rats received the oral administration of LH (20 mg/kg). The blood glucose level, body weight, organ weight (renal and pancreas), and biochemical parameters were estimated. We also scrutinized the effect of LH to enhance intestinal barrier function and suppress inflammation and intestinal permeability. LH significantly (p < 0.001) decreased the glucose level and enhanced the body weight with a reduction of renal weight and boost the pancreas weight. LH significantly (p < 0.001) enhanced the creatinine level and decreased the albumin level, urine volume, urinary albumin excretion rate, and urinary albumin creatinine ratio in the urine. It also suppressed the renal parameters, such as creatinine, blood urea nitrogen, and urea. LH significantly (p < 0.001) altered the level of lipid and antioxidant parameters. LH treatment significantly (p < 0.001) suppressed the cytokines and inflammatory parameters. LH considerably enhanced the Ruminococcaceae, Blautia, and suppressed the abundance of Bifidobacterium, Clostridium, and Turicibacter. It reduced the F/B ratio along with alteration of community abundance of Firmicutes, Actinobacteria, Proteobacteria, Tenericutes, other bacteria, and Bacteroidetes. The current result suggests that LH suppressed the diabetic nephropathological condition via alteration of gut microbiota and inflammation.

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Section: Discussionmentioning

confidence: 99%

Lycoperoside H protects against diabetic nephropathy via alteration of gut microbiota and inflammation

Zhang

Liu

et al. 2022

J Biochem & Molecular Tox

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“…OPD exerted anti-obesity effect on HFD-induced metabolic syndrome mice through regulating gut microbiota, precisely, by reducing Firmicutes/Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels ( Chen S. et al, 2018 ). In addition, OPD played a protective role against renal damage in STZ-induced diabetic nephropathy through suppressing oxidative damage as evidence by the decreased level of MDA and increased activity of SOD, GSH, CAT, along with inflammatory response by reducing pro-inflammatory cytokines (IL-6, IL-1β) ( Qiao et al, 2020 ). Ruscogenin was also reported can improve diabetic nephropathy ( Lu et al, 2014 ).…”

Section: Pharmacological Properties Of Liriopogonsmentioning

confidence: 99%

Liriopogons (Genera Ophiopogon and Liriope, Asparagaceae): A Critical Review of the Phytochemical and Pharmacological Research

2021

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The closely related genera Liriope and Ophiopogon (Asparagaceae), collectively known in English as liriopogons, have similar therapeutic uses in treating cough, rheumatoid arthritis, and cleaning heat. The main aim of this review is to understand the current phytochemical and pharmacological knowledge including an assessment of the quality of the scientific evidence. A literature search was conducted in line with PRISMA guidelines, by retrieving available information up to 2020 from five online resources. The bioactive metabolites of liriopogons include steroidal saponins, flavonoids, polysaccharides, organic acids, phenols. Cardiovascular protective, anti-inflammatory, anti-diabetic, anti-oxidant, anti-cancer, neuroprotective, anti-viral, anti-acute myeloid leukemia and hepatoprotective effects have been at the center of attention. From a toxicological perspective Ophiopogon japonicus seems to be safe. Some problems with the quality of the pharmacological evidence stand out including the application of excessive dose level and methodological problems in the design. Additionally, a reasonable link between local/traditional uses and pharmacological assessment is often vague or not reflected in the text. Future researches on liriopogons are required to use rigorous scientific approaches in research on evidence-based natural products for the future benefits of patients.

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“…Ophiopogonin D (OP-D) is a steroidal glycoside from Ophiopogon japonicus. Recent studies have shown that OP-D is effective in diseases such as diabetic myocardial injuries , obesity (Chen Y. et al, 2018), osteoporosis (Huang et al, 2015), atopic dermatitis (An et al, 2020), and has a distinct anti-oxidant and anti-inflammatory effect (Ma et al, 2018;Qiao and Jiao 2020). OP-D has also been shown to have anti-cancer effects by controlling various signaling pathways in lung cancer (Lee et al, 2018a;Lee et al, 2018b), breast cancer (Zang et al, 2016;Zhu, Wang, and Chen 2020), and prostate cancer (Lu et al, 2018;Lu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Ophiopogonin D increase apoptosis by activating p53 via ribosomal protein L5 and L11 and inhibiting the expression of c-Myc via CNOT2

Jee

Lee

et al. 2022

Front. Pharmacol.

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Ophiopogonin D (OP-D), which is extracted from the root tuber of Ophiopogon japonicus, is well known for its anti-inflammatory, anti-oxidant, and anti-cancer effects. It is also therapeutic for various diseases such as diabetic myocardial injuries, obesity, atopic dermatitis, and osteoporosis. However, there are insufficient reports on the anti-cancer effects and molecular mechanisms of OP-D in colorectal cancer. Therefore, this study aimed to investigate the anti-cancer-modulating effect of OP-D on colorectal cancer. The study proved that OP-D (20–40 uM) has significant cell viability inhibition and anti-proliferative effects in Cell Counting Kit-8 (CCK-8) assay and colony formation assay. In addition, our immunofluorescence analysis data showed that OP-D (40 uM) inhibited the expression of Ki67, a cell proliferation marker, and confirmed that OP-D could induce nucleolar stress by depletion of IPO7 and XPO1. Furthermore, our western blot data showed that OP-D induced p53 expression via ribosomal protein (RP) L5 or L11 and inhibited c-Myc expression through CNOT2 in a dose-dependent manner. Additionally, OP-D regulated cyclin D1 and CDK4, which are well known as cell cycle regulatory proteins. OP-D consistently inhibited the phosphorylation of AKT expression in a dose-dependent manner. Furthermore, OP-D shortened c-Myc’s half-life in a time-dependent manner. Furthermore, CNOT2 knockdown enhanced the inhibitory effect of OP-D on c-Myc in colon cancer cells. Besides that, we confirmed that OP-D has a combinational anti-cancer effect of 5-FU or doxorubicin to reduce cell viability and induce apoptosis through p53 and c-Myc regulation. Altogether, our results suggest that OP-D regulates colon cancer cell proliferation and induces apoptosis by inhibiting c-Myc expression via activation of p53 and CNOT2 regulation. The study demonstrated that OP-D may be a promising natural anti-cancer agent for the treatment of colorectal cancer.

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Ophiopogonin D of Ophiopogon japonicus ameliorates renal function by suppressing oxidative stress and inflammatory response in streptozotocin-induced diabetic nephropathy rats

Cited by 19 publications

References 33 publications

Lycoperoside H protects against diabetic nephropathy via alteration of gut microbiota and inflammation

Lycoperoside H protects against diabetic nephropathy via alteration of gut microbiota and inflammation

Liriopogons (Genera Ophiopogon and Liriope, Asparagaceae): A Critical Review of the Phytochemical and Pharmacological Research

Ophiopogonin D increase apoptosis by activating p53 via ribosomal protein L5 and L11 and inhibiting the expression of c-Myc via CNOT2

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