miR-378a-5p and miR-630 induce lens epithelial cell apoptosis in cataract via suppression of E2F3

Gao, Weiwei; Zhou, Xiaoqing; Lin, Ruihua

doi:10.1590/1414-431x20209608

Cited by 14 publications

(8 citation statements)

References 40 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 6 MiR‐378a‐5p and miR‐630 have been documented to induce cataract lens epithelial cell apoptosis by downregulating E2F3 expression. 7 Besides, miR‐630 functions as a tumor oncogene in renal cell carcinoma. 8 …”

Section: Introductionmentioning

confidence: 99%

“…Chen et al clarified that miR‐193a‐3p knockdown promoted ovarian cancer progression by targeting GRB7 and MAPK/ERK pathway 6 . MiR‐378a‐5p and miR‐630 have been documented to induce cataract lens epithelial cell apoptosis by downregulating E2F3 expression 7 . Besides, miR‐630 functions as a tumor oncogene in renal cell carcinoma 8 …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

MiR‐630 suppresses non‐small cell lung cancer by targeting vimentin

Wang

et al. 2022

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Objective This study aimed to clarify the function of miR‐630 on non‐small cell lung cancer (NSCLC) cells. Methods Quantitative real‐time PCR was utilized to detect the mRNA expression of miR‐630 and vimentin (VIM) in NSCLC tissues and cells. The protein expression of VIM, P53, Caspase‐3, Bcl‐2, Bax and JAK2/STAT3 was evaluated via Western blot. Dual‐luciferase reporter assay was applied to evaluate whether VIM is the target gene of miR‐630. The migration, invasion, proliferation and apoptosis of NSCLC cells were examined by wound‐healing assay, transwell assay, CCK‐8 assay, and flow cytometry, respectively. Results MiR‐630 was lowly expressed in NSCLC tissues and cells, while VIM was highly expressed in NSCLC cells. Dual‐luciferase reporter assay data validated that miR‐630 directly targeted VIM. MiR‐630 overexpression inhibited VIM expression, but the inhibition of miR‐630 upregulated VIM expression. Besides, miR‐630 mimics restrained cell migration, invasion, and proliferation, and promoted NSCLC cell apoptosis. Whereas, VIM overexpression partly attenuated the inhibitory effect of miR‐630 on NSCLC cells. Moreover, miR‐630 mimics impeded p‐JAK2 and p‐STAT3 protein expression; and miR‐630 inhibitor upregulated p‐STAT3 and VIM protein expression, which was reversed after the addition of STAT3 inhibitor C188‐9. Conclusion MiR‐630 constrained the progression of NSCLC by inhibiting JAK2/STAT3 pathway and downregulating VIM expression.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MiR‐630 suppresses non‐small cell lung cancer by targeting vimentin

Wang

et al. 2022

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

“…E2F3, which is an important cell cycle regulator and figures prominently in regulating cell proliferation, apoptosis and differentiation, belongs to the E2F transcriptional regulatory family and is closely related to tumorigenesis [40][41][42]. Reportedly, E2F3 expression is upregulated in tumors, such as osteosarcoma and breast cancer, and enhances the proliferation and colony formation of tumor cells [42,43].…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA MCM3AP antisense RNA 1 promotes non-small cell lung cancer progression through targeting microRNA-195-5p

Shen

Tao

et al. 2021

Bioengineered

View full text Add to dashboard Cite

Lung cancer (LC) ranks first among all causes of cancer-related death, with non-small cell lung cancer (NSCLC) taking up 85% of lung cancer cases. Although lncRNA MCM3AP antisense RNA 1 (MCM3AP-AS1) has been reported to be an oncogenic factor in NSCLC, its detailed mechanism in NSCLC is unknown. In this study, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine MCM3AP-AS1, microRNA (miR)-195-5p and E2F transcription factor 3 (E2F3) mRNA expressions in NSCLC tissues and cells. Western blot was utilized to determine the expression levels of E2F3, BCL2-associated X protein (Bax), B-cell lymphoma-2 (Bcl-2), E-cadherin and N-cadherin. CCK-8 and Transwell assays were conducted to examine cell proliferation, migration and invasion, respectively. Dual-luciferase reporter assay and RNA immunoprecipitation experiments were used to determine the regulatory relationships between MCM3AP-AS1 and miR-195-5p, and miR-195-5p and E2F3. We demonstrated that MCM3AP-AS1 was overexpressed in NSCLC tissues and cells, and MCM3AP-AS1 overexpression accelerated the proliferation, migration and invasion of NSCLC cells. In addition, MCM3AP-AS1 overexpression markedly up-modulated Bcl-2 expression and repressed Bax expression; MCM3AP-AS1 overexpression also significantly upregulated N-cadherin expression and suppressed E-cadherin expression in NSCLC cells. What is more, in NSCLC cells, miR-195-5p was a target of MCM3AP-AS1, and the latter worked as a molecular sponge for miR-195-5p to regulate E2F3 expression. Collectively, MCM3AP-AS1, serving as a competitive endogenous RNA (ceRNA) to regulate miR-195-5p/E2F3 axis, promotes NSCLC progression, which is a promising therapeutic target for NSCLC.

show abstract

“…E2F3, a key transcription factor, is frequently overexpressed in various cancers and thus contributes to human carcinogenesis (Gao et al, 2017). Moreover, E2F3 was reported to be downregulated in human cataract lens tissues (Gao & Zhou, 2020; Xiang et al, 2016). Here, we first identified that E2F3 was a functional target of miR‐222‐3p in regulating H 2 O 2 ‐induced HLE‐B3 cell damage.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circZNF292 regulates H₂O₂‐induced injury in human lens epithelial HLE‐B3 cells depending on the regulation of the miR‐222‐3p/E2F3 axis

Gao

et al. 2021

Cell Biology International

View full text Add to dashboard Cite

Circular RNAs (circRNAs) play important roles in the pathogenesis of age-related cataract (ARC). CircRNA zinc finger protein 292 (circZNF292, hsa_circ_0004058) is downregulated in ARC lens capsules. Here, we focused on its precise roles in oxidative stress underlying the pathogenesis of ARC. CircZNF292, microRNA (miR)-222-3p, and E2F transcription factor 3 (E2F3) were quantified by quantitative real-time polymerase chain reaction or western blot. Cell viability was assessed by the cell counting kit-8 assay. Cell cycle distribution and apoptosis were detected by flow cytometry. The activities of superoxide dismutase, catalase, and malondialdehyde were measured using the corresponding assay kit. Targeted correlations among circZNF292, miR-222-3p, and E2F3 were verified by the dual-luciferase reporter, RNA immunoprecipitation and RNA pull-down assays. Our data showed that circZNF292 was downregulated in ARC tissues and H 2 O 2 -treated human lens epithelial B3 (HLE-B3) cells. Increased expression of circZNF292 alleviated H 2 O 2 -induced cell viability suppression, apoptosis promotion, and oxidative stress enhancement. Mechanistically, circZNF292 directly targeted miR-222-3p, and circZNF292 regulated E2F3 expression through miR-222-3p. MiR-222-3p was a functional mediator of circZNF292 in modulating H 2 O 2 -induced injury in HLE-B3 cells. Furthermore, reduced level of miR-222-3p ameliorated H 2 O 2 -induced HLE-B3 cell damage by upregulating E2F3. Our present study demonstrated that increased expression of circZNF292 ameliorated H 2 O 2 -induced injury in HLE-B3 cells at least in part through the miR-222-3p/ E2F3 axis, highlighting a novel insight into the involvement of circRNAs in the pathogenesis of ARC.

show abstract

miR-378a-5p and miR-630 induce lens epithelial cell apoptosis in cataract via suppression of E2F3

Cited by 14 publications

References 40 publications

MiR‐630 suppresses non‐small cell lung cancer by targeting vimentin

MiR‐630 suppresses non‐small cell lung cancer by targeting vimentin

Long non-coding RNA MCM3AP antisense RNA 1 promotes non-small cell lung cancer progression through targeting microRNA-195-5p

Circular RNA circZNF292 regulates H₂O₂‐induced injury in human lens epithelial HLE‐B3 cells depending on the regulation of the miR‐222‐3p/E2F3 axis

Contact Info

Product

Resources

About

miR-378a-5p and miR-630 induce lens epithelial cell apoptosis in cataract via suppression of E2F3

Cited by 14 publications

References 40 publications

MiR‐630 suppresses non‐small cell lung cancer by targeting vimentin

MiR‐630 suppresses non‐small cell lung cancer by targeting vimentin

Long non-coding RNA MCM3AP antisense RNA 1 promotes non-small cell lung cancer progression through targeting microRNA-195-5p

Circular RNA circZNF292 regulates H2O2‐induced injury in human lens epithelial HLE‐B3 cells depending on the regulation of the miR‐222‐3p/E2F3 axis

Contact Info

Product

Resources

About

Circular RNA circZNF292 regulates H₂O₂‐induced injury in human lens epithelial HLE‐B3 cells depending on the regulation of the miR‐222‐3p/E2F3 axis