MicroRNA-629-5p promotes osteosarcoma proliferation and migration by targeting caveolin 1

Abstract

Osteosarcoma is a highly malignant tumor that occurs in the bone. Previous studies have shown that multiple microRNAs (miRNAs) regulate the development of osteosarcoma. This study aimed to explore the role of miR-629-5p and its target gene, caveolin 1 (CAV1), in osteosarcoma development. To analyze the expression of miR-629-5p and CAV1 mRNA in osteosarcoma tissues and cell lines, qRT-PCR analysis was performed. Dual-luciferase reporter experiments were subsequently performed to validate the relationship between CAV1 and miR-629-5p. CCK8 assay was used to measure osteosarcoma cell proliferation, and wound-healing assay was performed to study their migratory phenotype. Our findings revealed that miR-629-5p was overexpressed in osteosarcoma tissues and cells, and thereby enhanced cell proliferation and migration. Further, we validated that miR-629-5p targets CAV1 mRNA directly. CAV1 expression, which was negatively correlated with miR-629-5p expression, was found to be downregulated in osteosarcoma tissue samples. Moreover, our data showed that an increase in CAV1 level led to a decline in osteosarcoma cell proliferation and migration, which could be rescued by miR-629-5p upregulation. Overall, our study confirmed that miR-629-5p promoted osteosarcoma proliferation and migration by directly inhibiting CAV1.

Overexpression of Long Non-Coding RNA MIR22HG Represses Proliferation and Enhances Apoptosis via miR-629-5p/TET3 Axis in Osteosarcoma Cells

“…In this study, we explored the effect of MIR22HG in osteosarcoma. In addition, it has been reported that miR-629-5p promoted osteosarcoma proliferation and migration by targeting caveolin 1 [ 9 ]. To confirm the potential interaction between MIR22HG and miR-629-5p, we first downloaded the data concerning the putative binding sites between MIR22HG and miR-629-5p from Starbase ( Fig.…”

Distinct Expression Patterns of Genes Coding for Biological Response Modifiers Involved in Inflammatory Responses and Development of Fibrosis in Chronic Hepatitis C: Upregulation of SMAD-6 and MMP-8 and Downregulation of CAV-1, CTGF, CEBPB, PLG, TIMP-3, MMP-1, ITGA-1, ITGA-2 and LOX

“…More recently, Baiocchini et al, (2019) described different patterns of CAV-1 expression on LSEC and hepatocytes in the liver of HCV-infected persons vs. healthy controls, as well as a significant association between the nuclear expression of CAV-1 in hepatocytes and the stage of fibrosis [20]. Interestingly, Gao et al, (2021) showed that miR-629-5p promotes osteosarcoma proliferation and migration via the direct inhibition of CAV-1 mRNA expression [21]. In our study, we noticed that the expression of CAV-1 was downregulated when we compared F1/F2 stages of fibrosis with healthy controls and in F3/F4 stages of fibrosis compared with healthy controls.…”

MiR-629-5p Promotes Prostate Cancer Development and Metastasis by Targeting AKAP13

“…By combining clinical samples with public databases, we found that miR-629-5p increased to varying degrees in primary localized PCa tissues (PPCa) and metastatic PCa tissues (MPCa) compared with adjacent normal tissues (ANT), and bioinformatics analysis suggested that high level of miR-629-5p was related to poor prognosis. Although miR-629-5p has proved to play a role in promoting tumor growth and metastasis in different types of tumors such as colorectal cancer, hepatocellular carcinoma, lung adenocarcinoma and osteosarcoma (11)(12)(13)(14). However, the exact mechanism of its role in PCa metastasis remains unknown.…”